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Gene sequencing chip and system

A gene sequencing and chip technology, which is applied in the measurement/inspection of microorganisms, biochemical instruments, biochemical equipment and methods, etc., can solve the problems of difficulty in preparing waveguide chips and high cost

Pending Publication Date: 2021-05-11
CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

[0003] In order to solve the problems of difficulty in preparing waveguide chips for gene sequencing and high cost, the present invention provides a gene sequencing waveguide chip for single-molecule fluorescence sequencing

Method used

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  • Gene sequencing chip and system
  • Gene sequencing chip and system
  • Gene sequencing chip and system

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Embodiment Construction

[0024] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, but not to limit the present invention.

[0025] The present invention provides a gene sequencing system, such as figure 1 As shown, the gene sequencing system of the present invention includes an excitation light source 1, a light splitting element 2, a gene sequencing chip 3, and a detection unit 4; A corresponding connection transmits the excitation light to the site of the polymerase chain reaction; the detection unit 4 is aligned with the nanopore 303 on the gene sequencing chip 3, and the fluorescent signal generated by the polymerase chain reaction in the nanopore 303 is displayed on the detection unit 4 internal imagi...

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Abstract

The invention belongs to the technical field of biological detection, and provides a gene sequencing chip and system. The gene sequencing chip comprises an optical fiber used for transmitting exciting light, at least one part of a cladding of the optical fiber is removed to form a D-shaped cross section, a nanopore is formed in the removal position by modification, and the nanopore is located in an evanescent field of the optical fiber. By means of the nanopore structure, physical localization of a nucleic acid fragment to be sequenced and a sequencing enzyme is realized, a laser enters the single optical fiber of the chip through coupling of a light splitting element, the evanescent field generated on the upper surface of the optical fiber excites a free basic group carrying a fluorescent dye in the nanopore, and a generated fluorescence sequence signal is detected in a microscopy imaging mode.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a gene sequencing chip and system for single-molecule fluorescence sequencing. Background technique [0002] Sequencing technology based on fluorescent labeling method is an important means to achieve high-throughput single-molecule sequencing. The key to achieving high signal-to-noise ratio collection of single-molecule fluorescence is to reduce background noise. There are currently two effective ways to realize single-molecule fluorescence excitation. (1) Reducing the number of molecules in the illumination area through nanopore confinement and constraining the volume of excitation light through the formed zero-mode waveguide effect, thereby realizing single-molecule fluorescence excitation (2) based on The evanescent field generated by total internal reflection excites single-molecule fluorescence, and both techniques are currently used in commercial sequencing i...

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Application Information

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IPC IPC(8): C12M1/40C12M1/34C12M1/00B82Y40/00B82Y15/00
CPCC12Q1/6874B82Y40/00B82Y15/00
Inventor 王越吴一辉周文超
Owner CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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