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Preparation method of phellinus igniarius active ingredient and application of phellinus igniarius active ingredient in medicine or food for assisting in reducing blood sugar

An active ingredient and blood sugar-lowering technology, applied in the biological field, can solve the problems of less alcohol-soluble components and no comparability, and achieve the effect of lowering blood sugar

Active Publication Date: 2021-05-14
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phellinus polysaccharides are extracted from the Phellinus fruiting body or Phellinus mycelia through alcohol precipitation, but there are few studies on alcohol-soluble components. Only CN111096983A patent mentions the use of mycelia alcohol The hypoglycemic activity of the soluble fraction was studied, but Ph. Phellinus ignarius with Phellinus S. sanghuang They are species of different genera, not comparable
There is no report on the alcohol-soluble active ingredients extracted from the fermentation broth after the Phellinus mycelium is filtered out

Method used

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  • Preparation method of phellinus igniarius active ingredient and application of phellinus igniarius active ingredient in medicine or food for assisting in reducing blood sugar
  • Preparation method of phellinus igniarius active ingredient and application of phellinus igniarius active ingredient in medicine or food for assisting in reducing blood sugar
  • Preparation method of phellinus igniarius active ingredient and application of phellinus igniarius active ingredient in medicine or food for assisting in reducing blood sugar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] 1. Materials and methods

[0063] 1.1 Materials

[0064] 1.1.1 Test samples

[0065] Phellinus strain, preserved by the Key Laboratory of Applied Fungi of Shandong Province.

[0066] The bacterial classification that this application adopts is obtained by tissue separation and bacterial classification purification after collecting fresh sporocarp from the mulberry live standing tree in the mountainous area of ​​Anshun City, Guizhou Province, and this bacterial strain is classified and named Phellinus ( Sanghuang porus sanghuang ), which was deposited in the General Microorganism Center of China Committee for the Collection of Microbial Cultures on January 4, 2021, with the preservation number CGMCCNO.21068.

[0067] Morphological characteristics of fruiting bodies: Basidiocarp perennial, sessile cap-shaped, usually solitary, sour when fresh, corky, woody after drying; caps horseshoe-shaped, protruding outwards up to 5 cm, 11 cm wide, 6 cm thick at the base ;The surfa...

Embodiment 2

[0197] In the present embodiment, the preparation method of the Phellinus fermented liquid alcohol-dissolved sample is the same as that in Example 1, and the extractant is different: the Phellinus fermented liquid alcohol-dissolved sample is respectively mixed with petroleum ether and chloroform volume ratio 1:1 mixed solvent, chloroform and The mixed solvent of ethyl acetate volume ratio 2:3, the mixed solvent extraction of sherwood oil, chloroform, ethyl acetate and n-butanol volume ratio 2:2:3:3, obtain 1:1 mixed solvent extract 1,2: 3 mixed solvent extract 2, 2:2:3:3 mixed solvent extract 3.

Embodiment 1

[0199] On the basis of the study in Example 1, the extractant for extracting the active components of Phellinus phellidis fermentation broth has been optimized. In order to further improve the extraction efficiency, the test of this example was carried out. During the test, various solvents and various proportions of mixed solvents were selected. In order to save the length of the description, only mixed solvents and proportions with better effects are listed here. By this test, it is found that the Phellinus fermented liquid alcohol-dissolving sample is respectively with the mixed solvent of sherwood oil and chloroform volume ratio 1:1, the mixed solvent of chloroform and ethyl acetate volume ratio 2:3, sherwood oil, chloroform, ethyl acetate The mixed solvent extraction with n-butanol volume ratio of 2:2:3:3, compared with the single petroleum ether, chloroform, ethyl acetate and n-butanol extractant, has higher extraction efficiency and better effect of active ingredients. ...

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Abstract

The invention discloses a preparation method of a phellinus igniarius active ingredient, which comprises the following steps: taking a phellinus igniarius strain (Sanghuangporus sanghuang) fermentation liquor with the preservation number of CGMCC NO.21068, filtering mycelia, separating to obtain a phellinus igniarius fermentation liquor, heating and concentrating, adding 95% ethanol for soaking treatment, centrifuging, collecting supernate, recycling ethanol by using a rotary evaporator, collecting an alcohol-soluble substance, adding water for dissolving, freezing at -20 DEG C, performing vacuum freeze drying treatment, and crushing to obtain a phellinus igniarius fermentation liquor alcohol-soluble sample. The phellinus igniarius fermentation liquor alcohol-soluble sample is subjected to fractional extraction by using four organic solvents to obtain an extract. The research result provides a certain test basis and theoretical basis for subsequent development and utilization of phellinus igniarius liquid fermentation active substances and research and development of food, health care products or medicines related to blood sugar reduction and the like, and has certain theoretical significance and practical application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of active ingredients of Phellinus Phellinus and its application in auxiliary hypoglycemic drugs or food. Background technique [0002] Phellinus Sanghuang porus sanghuang It is a precious perennial large medicinal fungus, usually born in the genus Morus ( Morus L. ) plant, also known as Sang'er, Sangchen, and Phellinus, known as "forest gold", is a kind of precious Chinese herbal medicine. Abdominal astringent pain, anal prolapse and diarrhea, leukorrhea, amenorrhea embolism treatment. [0003] As experts at home and abroad have more and more in-depth research on the anti-cancer mechanism and medicinal value of Phellinus, Phellinus has been widely recognized by people. Scientific research has proved that Phellinus polysaccharides can improve human immunity and inhibit the growth of tumor cells. Proliferation, scavenging free radicals, preventing ...

Claims

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Application Information

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IPC IPC(8): A61K36/07A61P3/10A61P3/06A23L33/10
CPCA61K36/07A61P3/10A61P3/06A23L33/10A61K2236/19A61K2236/35A61K2236/39A23V2002/00A23V2200/3262A23V2200/328
Inventor 田雪梅张国利李喆
Owner QINGDAO AGRI UNIV
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