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Bivalent live vaccine for bovine viral diarrhea and infectious bovine rhinotracheitis and preparation method of bivalent live vaccine

A technology for bovine viral diarrhea and rhinotracheitis, applied in biochemical equipment and methods, viruses, vaccines, etc., can solve the problems of few vaccines, expensive vaccines, and inability to obtain them quickly

Pending Publication Date: 2021-05-18
华威特(江苏)生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Inactivated vaccines induce short-lived immune responses, require multiple vaccinations, and fail to induce cytotoxic T lymphocyte responses
At present, there are very few commercialized vaccines in my country, and the cattle industry is facing a huge threat. Imported vaccines are expensive and cannot be obtained quickly when an epidemic occurs

Method used

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  • Bivalent live vaccine for bovine viral diarrhea and infectious bovine rhinotracheitis and preparation method of bivalent live vaccine
  • Bivalent live vaccine for bovine viral diarrhea and infectious bovine rhinotracheitis and preparation method of bivalent live vaccine
  • Bivalent live vaccine for bovine viral diarrhea and infectious bovine rhinotracheitis and preparation method of bivalent live vaccine

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Embodiment Construction

[0030] The present invention will be described in detail below in conjunction with the accompanying drawings and examples.

[0031] 1. Vaccine preparation

[0032] 1. Seedling strains

[0033] Bovine viral diarrhea / Mucosal Disease (Bovine viral diarrhea / Mucosal Disease, BVD / MD) NMM strain and bovine infectious rhinotracheitis (Infectious bovine rhinotracheitis, IBR) JSM strain are both preserved by Warwick (Jiangsu) Biopharmaceutical Co., Ltd. .

[0034] Among them, the type I bovine viral diarrhea virus strain was collected from a cattle farm in Inner Mongolia that was suspected to be sick (positive by PCR). One strain of the virus was isolated after the serum was inoculated with MDBK cells. It was detected by RT-PCR, gene sequencing, and neutralization. Test and immunofluorescence test, confirmed that the virus is a BVDVI type virus, named BVDV1 NM01 strain. In order to obtain virus seeds for seedling production, the isolated BVDV1 NM01 strain was continuously passaged on...

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Abstract

The invention discloses preparation and application of a bivalent live vaccine for bovine viral diarrhea and infectious bovine rhinotracheitis, and belongs to the technical field of animal vaccines and veterinary biological products. The vaccine comprises a live bovine viral diarrhea virus NMM strain and an infectious bovine rhinotracheitis virus JSM strain. The preparation of the vaccine comprises the following steps of 1) obtaining two effective strains and carrying out attenuated culture; 2) carrying out suspension culture on MDBK cells to respectively prepare virus antigens; and (3) proportioning according to the concentration of a titer of 10<4.7>TCID<50>, sub-packaging, adding 20% of a freeze-drying protective agent, and freeze-drying. According to the bivalent live vaccine for bovine viral diarrhea and infectious bovine rhinotracheitis, the same immune effect of inactivated vaccines with respective virus titers of 10<7.3>TCID<50> and 10<7.8>TCID<50> can be obtained under the concentration of 10<3.5>TCID<50>, and the results of safety evaluation and potency test evaluation of over-dose inoculation show that the bivalent live vaccine for bovine viral diarrhea and infectious bovine rhinotracheitis has the characteristics of low cost, high efficacy and safe inoculation.

Description

technical field [0001] The invention belongs to the technical field of animal vaccines and veterinary biological products, and in particular relates to a dual live vaccine of bovine viral diarrhea and bovine infectious rhinotracheitis and its preparation method and application. Background technique [0002] Bovine viral diarrhea / mucosal disease (Bovine viral diarrhea / Mucosal Disease, BVD / MD) is caused by viral diarrhea virus (Bovine viral diarrhea virus, BVDV), the clinical manifestations are diarrhea, fever, leukopenia, mucosal erosion and ulcer, Diarrhea or cough is an infectious disease that can cause persistent BVDV infection in cattle. Most persistently infected animals appear healthy, but their production performance declines and become an important source of infection. It poses a greater threat to my country's breeding industry and is It has caused serious economic losses and is one of the important pathogens that endanger the cattle industry. The disease was first disc...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61K39/12A61K39/245A61P31/14A61P31/22C12N7/08
CPCA61K39/12A61P31/14A61P31/22C12N7/00A61K2039/70A61K2039/552A61K2039/5254C12N2770/24334C12N2710/16034C12N2770/24352C12N2710/16052C12N2770/24364C12N2710/16064
Inventor 闫喜军孟庆森李真光和彦良陈超阳宋妮季烨王宇菲武华
Owner 华威特(江苏)生物制药有限公司
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