Bioactive peptide GLNMCRQCF as well as preparation method and application thereof

A bioactive peptide, active technology, applied in the field of protein

Inactive Publication Date: 2021-05-18
ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the function of the polypeptide fragment of 40S ribosomal protein S29 protein in the prior art

Method used

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  • Bioactive peptide GLNMCRQCF as well as preparation method and application thereof
  • Bioactive peptide GLNMCRQCF as well as preparation method and application thereof
  • Bioactive peptide GLNMCRQCF as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The artificial synthesis of embodiment 1 active peptide GLNMCRQCF

[0034] 1. Synthesis of Bioactive Peptides

[0035] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak with 50ml of dichloromethane (DCM).

[0036] 2. After 2 hours, wash the resin with nitrogen-dimethylformamide (DMF) that is 3 times the volume of the resin, and then drain it. Repeat this four times, and drain the resin for use.

[0037] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.

[0038] 4. Take a small amount of resin and test it with ninhydrin (Ninhydrin Nine Wells) method (two drops each of test A and test B, react at 100°C for 1 min), if the resin has color, it means that the deprotection is successful.

[0039] 5. Weigh an a...

Embodiment 2

[0075] The immune activity experiment of embodiment 2 bioactive peptides

[0076] 1. The experiment of bioactive peptide GLNMCRQCF promoting macrophages to secrete cytokines (ELISA method)

[0077] 1. Experimental reagents and instruments:

[0078] Reagents: Experimental animal balb / c mice (male 6, 8 weeks old), Shanghai Slack Experimental Animal Co., Ltd.; mouse lymphocyte extract, Shanghai Suo Laibao Biotechnology Co., Ltd.; RPMI1640 medium, GIBCO Company; cattle Serum albumin (bovine serum albumin, BSA), Genebase Company; The mouse spleen lymphocyte-derived bioactive peptide GLNMCRQCF obtained in Example 1; ELISA cytokine rapid kit (TNF-α, IL-1β and IL-6) , Wuhan Boster Biological Engineering Co., Ltd.

[0079] Instruments and equipment: LRH, 250F biochemical incubator Shanghai Heng Technology Co., Ltd.; GL, 22M high-speed refrigerated centrifuge Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.; Hera cell 150CO2 incubator Heraeus Company; Dragon WellscanMK3 microplate r...

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Abstract

The invention relates to the field of protein, in particular to a bioactive peptide GLNMCRQCF as well as a preparation method and application thereof. Amino acid sequence of the bioactive peptide GLNMCRQCF is Gly-Leu-Asn-Met-Cys-Arg-Gln-Cys-Phe. An in-vitro immunomodulatory function experiment verifies that the bioactive peptide GLNMCRQCF has a very good immunomodulatory function. The bioactive peptide GLNMCRQCF can remarkably promote in-vitro proliferation of lymphocytes, improve the immunity of an organism, reduce the incidence rate of the organism, promote activation of macrophages, release cytokines and improve the quality of life, and has very important significance on the development of food, health care products and medicines with immunomodulatory functions.

Description

technical field [0001] The present invention relates to the protein field, in particular to a bioactive peptide GLNMCRQCF and its preparation method and application. Background technique [0002] In recent years, bioactive peptides have become a familiar term. Because of its many potential biological functions, it has attracted more and more attention and has become one of the hot spots of scientific research. At present, the beneficial effects of many bioactive peptides have also been well proven, such as anti-cancer, lowering blood pressure, antibacterial, lowering cholesterol, anti-diabetic and other properties. More than 3000 different bioactive peptides have been reported in the current most authoritative bioactive peptide database BIOPEP-UMW. [0003] Immunomodulatory peptides are a class of bioactive peptides obtained from milk for the first time after the discovery of opioid peptides and their physiological activity has been proved. In 1981, Jolles et al. first di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C12N15/12A61K38/17A61K8/64A61Q19/00A61Q19/08A61P37/02A61P29/00A61P39/06A23L33/18
CPCC07K14/47A61K8/64A61Q19/00A61Q19/08A61P37/02A61P29/00A61P39/06A23L33/18A61K38/00A23V2002/00A23V2200/324A23V2200/30A23V2250/55
Inventor 张少辉张伯宇占文静
Owner ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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