Lactobacillus plantarum and application thereof in preparation of medicine or food for relieving diseases caused by hyperlipidemia
A technology of Lactobacillus plantarum, hyperlipidemia, applied in the direction of lactobacillus, cardiovascular system diseases, function of food ingredients, etc., can solve the problems of large side effects and high cost
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0026]Example 1 Isolation and Identification of Plant Lactobacillus HH19058
[0027]1, separation and purification and morphological identification
[0028]Kimchi juice: In September 2018, the cabbage dishes in Qingdao area, Shandong, collected a 10-fold gradient of the kimchi juice obtained by hiking and juice together.
[0029]The lactic acid bacteria in which the separated lactic acid bacteria were cultured in the anaerobic workstation by MRS solid medium, the LBS solid medium and NPNL solid medium, and the separated lactic acid bacteria were cultured in anaerobic working station for 48 h, and the typical colonies on the plate were separated. The colonies were studied by Gram staining, and at 37 ° C for 24 hours at 37 ° C in MRS liquid medium, 4 ° C refrigerators were refrigerated.
[0030]Such asfigure 1 withfigure 2 As shown, the strain HH19058 is purified to form a single bacteria in the MRS medium, and the colonies are almost consistent, most of which are circular, white, and surface smo...
Example Embodiment
[0056]Example 2 Study on Lactobacillus HH19058 Ledolesterol and Glyceride Trial
[0057]Plant Lactobacillus HH19058 was inoculated from 3% inoculation to a cholesterol medium (MRS-TC) at 37 ° C for 24 hours, and 0.2 ml of culture solution was taken, 4.8 ml of anhydrous ethanol oscillation was mixed with mixed 1 min, and retained 5 min Either evenly, 4000 r / min centrifuged for 10 min, and 2.0 mL of the supernatant was used for cholesterol assays, as a controlled cholesterol medium.
[0058]Plant Lactobacillus HH19058 was inoculated at 37 ° C for 24 hours in a 3% inoculated amount (MRS-Tg) for 24 hours, 4000 r / min centrifuged for 10 min, and 2.0 mL of the supernatant for the determination of triglycerides, The triglyceride medium of bacilli is carried out as a control.
[0059]Cholesterol medium (MRS-TC) formulation: 0.1 g of cholesterol, 0.1 g of sucrose ester, 5 ml of ice acetate, ultrasonic crushing, added to the MRS liquid medium, so that the concentration of cholesterol in the medium ...
Example Embodiment
[0062]Example 3 Effect of Plant Lactobacillus HH19058 on hyperlipidemia mice
[0063]After the selection of qualified C57 108 adult mice, 13 weeks old, male and female, of the same sex in the weight difference should be within 20% of average body weight, fed with the basic feed adaptive one week, the average body weight between the groups no Significant differences were divided into 3 groups (Table 2). The first group of normally feeding the basic feed, the second, third group fed high-fat feed, and high blood lipid mouse model was established by feeding 4 weeks high-fat feed.
[0064]Table 2 animal modeling packet
[0065]
[0066]After the model was established, the mice were allowed to irry the mice in the treatment method in Table 2, 4 weeks in Table 2, and intravenously intravascate at 0.1 ml / 10g. The weight of each 7 days is given once every 7 days, and the gastric volume is adjusted.
[0067]Plant Lactobacillus HH19058 bacterial solution, plant Lactobacillus HH19058 inoculated MRS broth c...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap