Method for preparing apigenin by utilizing biological enzymolysis

A technology of apigenin and biological enzymes, applied in organic chemistry, fermentation, etc., can solve the problems of complex process, low yield, high energy consumption, etc., and achieve the effect of simple and convenient operation

Active Publication Date: 2021-05-18
LIANYUAN KANGLU BIOLOGICAL TECH CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a method for preparing apigenin by using biological enzymolysis to solve the problem of complex process, low yield and high energy consumption in the existing apigenin preparation method technical issues

Method used

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  • Method for preparing apigenin by utilizing biological enzymolysis
  • Method for preparing apigenin by utilizing biological enzymolysis
  • Method for preparing apigenin by utilizing biological enzymolysis

Examples

Experimental program
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Embodiment 1

[0044] Utilize the method for preparing apigenin by biological enzymolysis, comprising the following steps:

[0045] S1. Enzymatic hydrolysis: Add 100g rhotropin and 2L 25% ethanol solution to the reaction vessel, mix well, heat in a water bath to 80°C, keep it warm for 10min, then cool to 60°C, add glacial acetic acid to adjust the pH value to 4.5, add 10mL compound enzyme for enzymolysis reaction, the compound enzyme includes activated rhamnosidase solution and activated glucosidase, the enzyme specific activity of rhamnosidase solution and glucosidase solution is 12000:10000, the enzymatic hydrolysis temperature of compound enzyme The enzymolysis reaction time is 4 hours at 55°C. After the enzymolysis reaction for 2 hours, the enzymolysis reaction solution is subjected to high-performance liquid chromatography detection at intervals of 1 hour. If the area of ​​the rhozoside absorption peak is less than 1%, it is judged that the reaction is complete, and the temperature is ra...

Embodiment 2

[0050] Utilize the method for preparing apigenin by biological enzymolysis, comprising the following steps:

[0051] S1. Enzymolysis: Add 100g rhotropin and 1.5L 30% ethanol solution to the reaction vessel, mix well, heat in a water bath to 80°C, keep warm for 15min, then cool to 60°C, add glacial acetic acid to adjust the pH value to 4, Add 12mL compound enzyme to carry out enzymolysis reaction, compound enzyme includes activated rhamnosidase liquid and activated glucosidase, the enzyme specific activity of rhamnosidase liquid and glucosidase liquid is 10000:10000, the enzymolysis of compound enzyme The temperature is 50°C, and the enzymolysis reaction time is 12 hours. After the enzymolysis reaction for 6 hours, the enzymolysis reaction solution is subjected to high performance liquid chromatography detection at intervals of 1 hour. If the area of ​​the rhozoside absorption peak is less than 1%, it is judged that the reaction is complete, and the temperature is raised to Ext...

Embodiment 3

[0056] Utilize the method for preparing apigenin by biological enzymolysis, comprising the following steps:

[0057] S1. Enzymolysis: Add 120g Rhodorin and 1.5L 30% ethanol solution to the reaction vessel, mix evenly, heat in a water bath to 80°C, keep warm for 10min, then cool to 60°C, add glacial acetic acid to adjust the pH value to 4.5, Add 15mL compound enzyme to carry out enzymolysis reaction, compound enzyme includes activated rhamnosidase liquid and activated glucosidase, the enzyme specific activity of rhamnosidase liquid and glucosidase liquid is 12000:10000, the enzymolysis of compound enzyme The temperature is 60°C, and the enzymolysis reaction time is 6 hours. After the enzymolysis reaction for 2 hours, the enzymolysis reaction solution is subjected to high performance liquid chromatography detection at intervals of 1 hour. If the area of ​​the rhotropin absorption peak is less than 1%, it is judged that the reaction is complete, and the temperature is raised to E...

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Abstract

The invention discloses a method for preparing apigenin by utilizing biological enzymolysis. The method comprises the following steps of: S1, enzymolysis: adding rhoifolin and water or a lower alcohol solution into a reaction container, uniformly mixing, heating to 70 DEG C or above, preserving heat, cooling to 60 DEG C or below, adding acid to adjust the pH value, adding a compound enzyme for an enzymolysis reaction, detecting whether the rhoifolin is completely reacted or not, and heating to extinguish the compound enzyme after the enzymolysis reaction is completed to obtain a reaction solution, wherein the compound enzyme comprises rhamnosidase and glucosidase; S2, separation: filtering the reaction solution to obtain apigenin coarse crystals, mixing the apigenin coarse crystals with an alcohol solution, and stirring until the apigenin coarse crystals are completely dissolved to obtain a complex solution; and S3, purification: cooling and crystallizing the complex solution, and filtering to obtain the apigenin. According to the method for preparing the apigenin by utilizing the biological enzymolysis, the rhoifolin is hydrolyzed into the apigenin by adopting biological enzyme catalysis, so that the method is easy and convenient to operate, green, pollution-free and suitable for large-scale production.

Description

technical field [0001] The invention relates to the field of apigenin, in particular to a method for preparing apigenin by biological enzymatic hydrolysis. Background technique [0002] Apigenin is a naturally occurring flavonoid compound that exists in a variety of plants in the form of plant yellow pigments. It is also found in yarrow. The molecular formula of apigenin is C 15 h 10 o 5 , molecular weight is 270, melting point is 345℃~350℃, insoluble in water, easily soluble in hot ethanol, dimethyl sulfoxide, dilute alkali and other solvents. It has biological activities such as dilating blood vessels, lowering blood pressure and lipids, anti-tumor, anti-inflammatory, anti-oxidation, and preventing various cancers. Because apigenin has good bioactivation, it has wide application prospects in the fields of functional food, medicine and cosmetics. [0003] There are two main methods for preparing apigenin: one is to extract directly from plants, and the other is to sem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/06C07D311/30
CPCC12P17/06C07D311/30
Inventor 肖业成魏有何刘冬敏廖深刻吴爱燕
Owner LIANYUAN KANGLU BIOLOGICAL TECH CO LTD
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