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Application of rice osrps6a gene or osrps6b gene in improving rice drought resistance

A drought resistance, rice technology, applied in application, genetic engineering, plant genetic improvement and other directions, to achieve the effect of potential application value

Active Publication Date: 2022-04-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, after the RPS6 phosphorylation site is mutated, the cell size will not be further affected by rapamycin, indicating that the phosphorylation of RPS6 is the key effector of mTOR regulating cell growth, and its deletion is equivalent to the inhibition of mTOR

Method used

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  • Application of rice osrps6a gene or osrps6b gene in improving rice drought resistance
  • Application of rice osrps6a gene or osrps6b gene in improving rice drought resistance
  • Application of rice osrps6a gene or osrps6b gene in improving rice drought resistance

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Experimental program
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Effect test

Embodiment 1

[0033] Example 1 Obtainment of rice osrps6a and osrps6b function-deficient mutants

[0034] In order to study the functions of the rice OsRPS6A gene and the homologous gene OsRPS6B, the OsRPS6A gene and the homologous gene OsRPS6B gene were edited using gene editing technology.

[0035] 1. Construction of CRISPR / Cas9 recombinant vector

[0036] 1.1 Using the sequence comparison analysis in the NCBI genome database, the Arabidopsis RPS6A gene sequence was used to find the homologous gene of this gene in rice, and two homologous genes were found in the rice genome. We named one of the genes OsRPS6A, Its CDS nucleotide sequence is shown in SEQ ID NO.1, and the encoded amino acid sequence is shown in SEQ ID NO.2; another gene is named OsRPS6B, and its CDS nucleotide sequence is shown in SEQ ID NO.3. The encoded amino acid sequence is shown in SEQ ID NO.4.

[0037] The amino acid sequences encoded by the rice OsRPS6A gene and OsRPS6B gene were compared with the RPS6 protein seque...

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Abstract

The invention discloses the application of the rice OsRPS6A gene and its homologous OsRPS6B gene in improving the drought resistance of rice and belongs to the field of biotechnology. The application includes: using CRISPR / CAS9 technology to reduce the expression or activity of the protein encoded by the rice OsRPS6A gene or OsRPS6B gene, thereby obtaining a rice mutant with enhanced drought resistance. The present invention uses CRISPR / Cas9 system to construct a plant recombinant expression vector for specific knockout of rice OsRPS6A gene and its homologous OsRPS6B gene target site, and obtains homozygous mutant plants with functionally deficient mutations of OsRPS6A gene or OsRPS6B gene through transgene , the invention provides a new direction for the study of rice drought resistance of rice OsRPS6A gene or OsRPS6B gene mutant plants, and has potential application value in agricultural development.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of rice OsRPS6A gene or OsRPS6B gene in improving rice drought resistance. Background technique [0002] Rice production is highly dependent on water resources. With the drastic changes in the global climate, the shortage of water resources has increasingly become an important factor affecting the stable production of rice. Drought stress affects the growth and production of rice. Therefore, it is an important means to solve the drought resistance of rice by excavating the genetic resources of drought resistance in rice and improving the drought resistance of rice through gene editing. [0003] The ribosome is an organelle that catalyzes protein synthesis and consists of a small 40S subunit and a large 60S subunit. RPS6 (ribosomal protein S6) belongs to the ribosomal protein family, which is very conserved in rice, Arabidopsis, human and yeast. There are two RPS6 ge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/29C12N15/55A01H5/00A01H5/10A01H6/46
CPCC12N15/8218C12N15/8273C07K14/415C12N9/22
Inventor 都浩葛洁瑜刘佳琦
Owner ZHEJIANG UNIV