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A kind of dna tetrahedron-resveratrol complex and its preparation method and use

A technology of resveratrol and tetrahedron, applied in the field of medicine, can solve the problems such as the therapeutic effect of tFNAs has not yet been found, and achieve good application prospects, improve insulin resistance, and reduce body weight and blood sugar levels.

Active Publication Date: 2022-07-05
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, tFNAs have not been found to have a therapeutic effect on insulin resistance

Method used

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  • A kind of dna tetrahedron-resveratrol complex and its preparation method and use
  • A kind of dna tetrahedron-resveratrol complex and its preparation method and use
  • A kind of dna tetrahedron-resveratrol complex and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1. Preparation and Characterization of DNA Tetrahedral Framework Nucleic Acids

[0036] 1. Preparation of DNA Tetrahedral Framework Nucleic Acids

[0037] DNA tetrahedral framework nucleic acids (tFNAs) are synthesized by self-assembly of four uniquely designed DNA single strands (S1, S2, S3, S4) through a PCR program (95°C for 10 min, then rapidly cooled to 4°C for more than 20 min). The specific preparation method is as follows:

[0038] Four DNA single strands S1, S2, S3 and S4 (the specific sequences are shown in Table 1) were dissolved in TM buffer (10 mM Tris-HCl, 50 mM MgCl) according to equimolar ratio 2 , pH 8.0), the final concentrations of the four DNA single strands were 1000 nM respectively, fully mixed and then heated to 95 °C for 10 min, then rapidly cooled to 4 °C and maintained for more than 20 min, that is, the DNA tetrahedral framework was obtained by self-assembly and synthesis Nucleic acids (tFNAs).

[0039] Table 1. Concrete sequences of...

Embodiment 2

[0044] Example 2. Preparation and characterization of the DNA tetrahedron-resveratrol complex of the present invention

[0045] 1. Preparation of DNA tetrahedron-resveratrol complex of the present invention

[0046]Preparation of tFNAs-RSV: The tFNAs synthesized in Example 1 and the resveratrol (RSV) solution were added to the TM buffer and mixed, so that the concentration of tFNAs in the mixed solution was 250 nM, and the concentration of RSV in the mixed solution was 80 μM, stirred on a magnetic stirrer at 4° C. for 6 h, and ultra-filtered at 4° C. under a centrifugal force of 6000 g for 5 min to obtain the DNA tetrahedron-resveratrol complex (tFNAs-RSV) of the present invention. The solvent of the resveratrol solution was DMSO at a concentration of 10 mM.

[0047] 2. Characterization of the DNA tetrahedron-resveratrol complex of the present invention

[0048] The successfully synthesized tFNAs-RSV were measured by UV spectrophotometer for absorption spectra, and TEM for m...

Embodiment 3

[0050] Example 3. Screening of the preparation process of the DNA tetrahedron-resveratrol complex of the present invention

[0051] tFNAs-RSV was prepared by the method described in Example 2, only the concentration of RSV in the mixed solution was changed (the concentrations of RSV in the mixed solution were 20 μM, 40 μM, 80 μM, 120 μM and 160 μM, respectively). The concentrations of tFNAs and RSV in each tFNAs-RSV were determined by NanoPhotometer N60, and the drug loading (LE) and encapsulation efficiency (EE) were calculated. Calculated as follows:

[0052] LE=(amount of total RSV material – amount of remaining RSV material) / (amount of total tFNAs material)

[0053] EE(%)=(amount of total RSV substance – amount of remaining RSV substance) / (amount of total RSV substance)×100%

[0054] The result is as image 3 It was shown that the most suitable drug loading and encapsulation efficiency could be obtained when the concentration of RSV was 80 μM due to the need for higher ...

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Abstract

The invention provides a DNA tetrahedron-resveratrol complex and a preparation method and application thereof, belonging to the technical field of medicine. The complex is a mixture of DNA tetrahedral framework nucleic acid and resveratrol. The DNA tetrahedron-resveratrol complex of the present invention can effectively reduce body weight and blood sugar content, improve the tolerance to glucose and insulin, and then effectively treat obesity and improve or even treat insulin resistance caused by obesity; the complex of the present invention can also be used in For the treatment of metabolic syndrome, type 2 diabetes, hypertension, hyperuricemia, dyslipidemia, cardiovascular and cerebrovascular diseases, polycystic ovary syndrome, Alzheimer's disease or tumors caused by insulin resistance. Meanwhile, the complex of the present invention can effectively treat adipose tissue inflammation. Compared with the single use of DNA tetrahedral framework nucleic acid or resveratrol, the complex of the present invention exerts a synergistic effect and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a DNA tetrahedron-resveratrol complex and a preparation method and application thereof. Background technique [0002] Metabolic syndrome is a common metabolic disease with glucose intolerance, hypertension and dyslipidemia as the main symptoms. Insulin resistance caused by obesity is a key cause of metabolic syndrome and an important risk factor for diseases such as type 2 diabetes and atherosclerosis. Chronic low-grade tissue inflammation is an important cause of obesity-induced insulin resistance. [0003] Resveratrol is a non-flavonoid polyphenol monomer in traditional Chinese medicine, and it is an antitoxin produced by many plants when they are stimulated. In vitro and animal experiments show that resveratrol has antioxidant, anti-inflammatory, anti-cancer and cardiovascular protection effects. However, resveratrol monomer has unstable properties, poor water ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7088A61K31/05A61K47/26A61K47/54A61P3/04A61P3/10A61P5/50A61P9/12A61P19/06A61P9/00A61P15/08A61P25/28A61P35/00
CPCA61K31/7088A61K31/05A61K47/26A61K47/549A61P3/04A61P3/10A61P5/50A61P9/12A61P19/06A61P9/00A61P15/08A61P25/28A61P35/00
Inventor 林云锋李彦静蔡潇潇
Owner SICHUAN UNIV
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