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Lactobacillus plantarum strain Y388 and application thereof

A technology of Lactobacillus plantarum, Y388, applied in the field of microorganisms

Active Publication Date: 2021-05-28
湖北均瑶大健康饮品股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Lactobacillus plantarum, which can produce high exopolysaccharides, has not been reported in this field

Method used

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  • Lactobacillus plantarum strain Y388 and application thereof
  • Lactobacillus plantarum strain Y388 and application thereof
  • Lactobacillus plantarum strain Y388 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 4

[0051] The indicator strains used in Experimental Example 4: Escherichia coli, Salmonella and Staphylococcus aureus are commercially available.

experiment example 6

[0052] The commercial fermented milk starter used in Experimental Example 6 contains live bacteria, and the commercial fermented milk starter is Beile Weikang probiotic yogurt starter, which is commercially available.

[0053] The culture medium used in the experimental example of the present invention:

[0054] MRS solid medium: peptone 10g, beef extract 10g, yeast extract 5g, diammonium hydrogen citrate 2g, glucose 20g, Tween 80 1mL, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.58g, manganese sulfate 0.25g , agar 15g, distilled water 1000mL, sterilized at 121°C for 20min, and adjusted to pH 6.8.

[0055] MRS liquid medium: peptone 10g, beef extract 10g, yeast extract 5g, diammonium hydrogen citrate 2g, glucose 20g, Tween 80 1mL, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.58g, manganese sulfate 0.25g 1. Distilled water 1000mL, sterilized at 121°C for 20min, and adjusted to pH 6.8.

[0056] Both LB medium and Columb...

experiment example 1

[0091] Screening of exopolysaccharide-producing strains in experimental example 1 biological sample

[0092] 1. Sample collection

[0093] Sampling from fermented food (pickles, cheese, fermented bean curd, yogurt, etc.), animal gastrointestinal tract, human feces, etc. The collected samples were packed into sample tubes and placed in foam boxes with ice packs, kept at a low temperature and brought back to the laboratory for separation.

[0094] 2. Sample pretreatment

[0095] Label and mark the collected biological samples; weigh 1 g of the samples respectively and put them into 15 mL centrifuge tubes filled with 9 mL of sterile water, and vibrate with a vortex shaker for 3 times, 1 min each time, with an interval of 15 s.

[0096] 3. Sample separation

[0097] Immediately after the shaking is completed, draw 100 μL of the suspension and add it to 900 μL of sterile water, shake and mix well, and complete one dilution; dilute 3 times in total to obtain 4 dilution gradients,...

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Abstract

The invention relates to a Lactobacillus plantarum strain Y388 and an application thereof, and belongs to the technical field of microorganisms. The preservation number of the Lactobacillus plantarum strain Y388 is CGMCC No. 21750. The Lactobacillus plantarum strain Y388 provided by the invention can be used for remarkably producing the exopolysaccharide, is sensitive to 14 common antibiotics, has a relatively strong inhibition effect on escherichia coli, staphylococcus aureus and salmonella, and is a safe, healthy and nutritional probiotic capable of producing the exopolysaccharide at high yield.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to a Lactobacillus plantarum strain Y388 and its application. Background technique [0002] Lactobacillus plantarum is a kind of lactic acid bacteria, anaerobic or facultative anaerobic, straight rod or curved rod, usually 0.9~1.2μm×3.0~8.0μm, single, paired or short chain, and its optimum growth temperature is 30-35°C, the optimum pH value is around 6.5, and the pH value 4.5-9.5 is the range of pH tolerance for the growth of Lactobacillus plantarum. Lactobacillus plantarum can tolerate low pH value and high-concentration salt solution, and use carbon source in the growth process Lactic acid is produced. Lactobacillus plantarum is a multifunctional probiotic that is abundant in the gastrointestinal tract. Lactobacillus plantarum and its metabolites in the gastrointestinal tract can produce specific physiological functions, such as immune regulation, functional barriers, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/127C12R1/25C12R1/225C12R1/46C12R1/01C12R1/23C12R1/245C12R1/645
CPCA23C9/127A23V2400/125A23V2400/123A23V2400/113A23V2400/249A23V2400/51A23V2400/169
Inventor 王均豪朱航明叶亚明
Owner 湖北均瑶大健康饮品股份有限公司
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