Lactobacillus plantarum strain Y388 and application thereof
A technology of Lactobacillus plantarum, Y388, applied in the field of microorganisms
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experiment example 4
[0051] The indicator strains used in Experimental Example 4: Escherichia coli, Salmonella and Staphylococcus aureus are commercially available.
experiment example 6
[0052] The commercial fermented milk starter used in Experimental Example 6 contains live bacteria, and the commercial fermented milk starter is Beile Weikang probiotic yogurt starter, which is commercially available.
[0053] The culture medium used in the experimental example of the present invention:
[0054] MRS solid medium: peptone 10g, beef extract 10g, yeast extract 5g, diammonium hydrogen citrate 2g, glucose 20g, Tween 80 1mL, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.58g, manganese sulfate 0.25g , agar 15g, distilled water 1000mL, sterilized at 121°C for 20min, and adjusted to pH 6.8.
[0055] MRS liquid medium: peptone 10g, beef extract 10g, yeast extract 5g, diammonium hydrogen citrate 2g, glucose 20g, Tween 80 1mL, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.58g, manganese sulfate 0.25g 1. Distilled water 1000mL, sterilized at 121°C for 20min, and adjusted to pH 6.8.
[0056] Both LB medium and Columb...
experiment example 1
[0091] Screening of exopolysaccharide-producing strains in experimental example 1 biological sample
[0092] 1. Sample collection
[0093] Sampling from fermented food (pickles, cheese, fermented bean curd, yogurt, etc.), animal gastrointestinal tract, human feces, etc. The collected samples were packed into sample tubes and placed in foam boxes with ice packs, kept at a low temperature and brought back to the laboratory for separation.
[0094] 2. Sample pretreatment
[0095] Label and mark the collected biological samples; weigh 1 g of the samples respectively and put them into 15 mL centrifuge tubes filled with 9 mL of sterile water, and vibrate with a vortex shaker for 3 times, 1 min each time, with an interval of 15 s.
[0096] 3. Sample separation
[0097] Immediately after the shaking is completed, draw 100 μL of the suspension and add it to 900 μL of sterile water, shake and mix well, and complete one dilution; dilute 3 times in total to obtain 4 dilution gradients,...
PUM
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