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Three-year-old peony rhizosphere bacillus megaterium FD3-13 for oil and application thereof

A technology of Bacillus megaterium and peony root, applied in the field of microorganisms, to achieve good results

Pending Publication Date: 2021-05-28
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the bacteria that decompose organophosphate in the rhizosphere of tree peony

Method used

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  • Three-year-old peony rhizosphere bacillus megaterium FD3-13 for oil and application thereof
  • Three-year-old peony rhizosphere bacillus megaterium FD3-13 for oil and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1 Isolation and purification of a three-year-old oil strain of Bacillus megaterium FD3-13 from the peony rhizosphere

[0080] Firstly, remove the visible debris on the rhizosphere soil surface of the three-year-old oil peony planted in the experimental farm of Kaiyuan Campus of Henan University of Science and Technology in Luoyang City, Henan Province. According to the five-point sampling method, the rhizosphere soil was collected with a soil drill at a distance of 10 cm from the plant. Put it into a sterile bag and store it in an ice box. After arriving at the laboratory, use a brush to brush off the soil attached to the root system, weigh 5g of fresh soil samples in the ultra-clean workbench into a sterile centrifuge tube, dilute to 50mL with sterile water, shake for 20min, and press 10 The dilutions were carried out sequentially, and a pipette was used to draw 10 -6 ~10 -4 Each 0.1mL sample of the three dilutions was evenly spread on the organophosphate sol...

Embodiment 2

[0082] Example 2 Identification of Moutan Rhizosphere Bacillus megaterium FD3-13 for three-year oil production

[0083] Bacterial genomic DNA was extracted using the AxyPrep Bacterial Genomic DNA Miniprep Kit. Genomic DNA was amplified using bacterial universal primers 27F: 5'-AGAGTTTGATCCTGGCTCAG-3' and 1492R: 5'-CTACGGCTACCTTGTTACGA-3' to obtain PCR products. PCR products were purified with AxyPrep DNA. The purified PCR products were bidirectionally sequenced using the ABI3730-XL sequencer at Shanghai Passino Biotechnology Co., Ltd. After using ChromasPro software to truncate the invalid sequence, use BioEdit software to splice the double-ended effective sequence, and then use the Blast program in the NCBI database to compare the effective sequence with the reference sequence in the NCBI database to obtain a sequence similar to the strain to be tested. The most sexual strain is Bacillus megaterium, with a homology of 99%.

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Abstract

The invention relates to the technical field of microorganisms, and particularly relates to a three-year-old peony rhizosphere bacillus megaterium FD3-13 for oil and application of the three-year-old peony rhizosphere bacillus megaterium FD3-13. According to the invention, the application of the strain in the organophosphorus degradation test results show that the content of the rapidly available phosphorus activated from the calcium phytate by the strain is 5.30 mg / L, and the activation rate of the calcium phytate reaches 62.14%. Therefore, the degradation effect is good.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Bacillus megaterium FD3-13 used for three-year-old oil in the peony rhizosphere and its application. Background technique [0002] Organophosphate-decomposing bacteria refer to bacteria that can decompose organic phosphorus compounds. There are various types of bacteria, and different bacteria have different phosphorus-dissolving mechanisms. The mechanism of microbial solubilization and mineralization of organic phosphorus is generally believed to be related to the enzymes it secretes. Organophosphate-decomposing bacteria can enzymatically hydrolyze organophosphate by secreting extracellular phosphatase. When phosphorus becomes the main limiting factor for microbial growth, microorganisms will release extracellular phosphatase to hydrolyze organic phosphorus into available phosphorus. At the same time, microbial intracellular alkaline phosphatase will be induce...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/11
CPCC12N1/20C05F11/08
Inventor 张兆沛黄文文程亚南王菲张影申长卫
Owner HENAN INST OF SCI & TECH