Method for treating and culturing primary placenta decidua parietalis mesenchymal stem cells

Abstract

The invention provides a method for treating and culturing primary placenta decidua parietalis mesenchymal stem cells, which comprises the following steps of: removing chorion tissues connected with the edge of in-vitro placenta tissues and tissues attached to the chorion tissues, and cleaning blood and blood clots; scraping decidua parietalis tissues, and conducting cleaning and cutting to form pieces; adding digestive enzyme for digestion; stopping digestion, filtering out tissue and placenta decidua parietalis mesenchymal stem cells, adding a tissue protection solution, and conducting cleaning and diluting; separating out supernate, adding red blood cell lysate, conducting centrifuging, and removing supernate; adding tissue protection liquid, conducting cleaning and diluting, adding hydroxyethyl starch and glutamine, conducting settling, sucking out supernate, removing red blood cells at the bottom, and conducting centrifuging; removing the supernatant, re-suspending the cell precipitate to obtain a cell suspension, and conducting inoculating and culturing; changing the liquid, and continuously conducting culturing; and conducting subculturing. The method is convenient to operate, the purity, the survival rate, the number and the activity of the primary placenta decidua parietalis mesenchymal stem cells can be effectively improved, and the culture time is shortened.

Description

technical field [0001] The invention belongs to the field of stem cells and regenerative medicine, and in particular relates to a method for processing and culturing primary placental wall decidua mesenchymal stem cells. Background technique [0002] Placenta decidua parietalis (PCDP) wall decidua mesenchymal stem cells (Placenta Decidua Parietalis, PCDP) wall decidua is one of the maternal components, and the wall decidua mesenchymal stem cells isolated from it can be used for transplantation or cryopreservation of the mother's own cells. sex. Decidual mesenchymal stem cells are different from other sources of mesenchymal stem cells in terms of phenotype, proliferation and cytokine expression, and have unique properties, such as low immunogenicity, which have attracted the attention of many scientists in recent years. Mesenchymal stem cells have broad application prospects in tissue repair such as endometrial reconstruction, pelvic organ prolapse, and bladder tissue repair...

AI Technical Summary

Problems solved by technology

At present, most of the existing collection and storage devices are complex in structure, cumbersome in operation, lack of professionalism, easy to damage, and risk of biohazard. The traditional method of processing and culturing primary placental wall decidua mesenchymal stem cells does not have any cooling and heat preservation measures during the transportation and isolation of the collected placenta, which cannot guarantee the safety of the placenta. Biological activity will not be conducive to the isolation of primary placental wall decidua mesenchymal stem cells; the placenta produced by pregnant women giving birth to fetuses in the early stage will be polluted during the preparation process due to possible bacterial infection in the birth canal of pregnant women, and it is easy to cause pollution during transportation , the cells are difficult to extract, the culture time is long, and the number of cells is small

At present, there is still a lack of a treatment and culture method that can improve the purity, quantity, activity and culture time of primary placental wall decidual mesenchymal stem cells

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