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Screening method of early-stage liver cancer diagnosis markers for people with liver cirrhosis and hepatitis

A diagnostic marker and early liver cancer technology, applied in the field of clinical medical diagnosis, can solve problems affecting the cure rate and prognosis of liver cancer, and achieve high accuracy

Active Publication Date: 2021-06-01
MEI HOSPITAL UNIV OF CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Early liver cancer has no specific clinical symptoms, no obvious positive signs or only signs similar to liver cirrhosis. When obvious clinical symptoms and signs appear, liver cancer is already in the middle and advanced stages, which greatly affects the cure rate and prognosis of liver cancer

Method used

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  • Screening method of early-stage liver cancer diagnosis markers for people with liver cirrhosis and hepatitis
  • Screening method of early-stage liver cancer diagnosis markers for people with liver cirrhosis and hepatitis
  • Screening method of early-stage liver cancer diagnosis markers for people with liver cirrhosis and hepatitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]Example 1. Screening of Early Diagnosis Markers of Hepatitis Hepatoma.

[0035]Studies: This study included 24 patients with plasma samples and 26 patients with primary liver cancer patients, and all plasma samples were placed in the -80 ° C refrigerator. When the plasma sample was taken out, after the sample pre-treatment, the metabolic analysis of the high-performance liquid chromatography spectrophotographic system was used, and the secondary mass spectrum was compared to the standard database, and the compound has been obtained by related software to obtain a compound name. And the raw data of the peak area. The compounds in the original data were then matched by the inline metabolic analysis platform MetaboAnalyst, and the non-metabolite component was filtered, and all sample original metabolite content matrices were finally obtained.

[0036]Import the original metabolite content matrix into SIMCA-P software for different metabolites: Application of orthogonal matrix least squ...

Embodiment 2

[0044]Example 2 Screening of early diagnostic markers in cirrhosis populations.

[0045]Studies: This study included 28 cases of liver-hardened population plasma samples and blood samples of patients with primitive liver cancer, and all plasma samples were centrifuged in the -80 ° C refrigerator. When the plasma sample was taken out, after the sample pre-treatment, the metabolic analysis of the high-performance liquid chromatography spectrophotographic system was used, and the secondary mass spectrum was compared to the standard database, and the compound has been obtained by related software to obtain a compound name. And the raw data of the peak area. The compounds in the original data were then matched by the inline metabolic analysis platform MetaboAnalyst, and the non-metabolite component was filtered, and all sample original metabolite content matrices were finally obtained.

[0046]Import the original metabolite matrix into SIMCA-P software for different metabolites for differentia...

Embodiment 3

[0053]Embodiment 3, specifically, step S1 also includes the following steps:

[0054]S11, sample collection, use anti-coagulation tube to acquire target venous blood, acquire the supernatant as plasma sample by centrifugation. Specifically, 1 ml of hepatocheese, hepatitis, liver cancer patients were used, respectively, and gently reverse the collection tube, and the blood was mixed, and the low temperature was low-speed for 8 minutes (within 1 h after blood sample collection), take the supernatant As a plasma sample.

[0055]S12, the sample preoperative treatment, the sample obtained by step S11 is mixed with the experimental solvent and centrifuged, and the supernatant is obtained. Specifically, 60 μl of plasma was mixed with 300 to 350 μL of acetonitrile tweet 50s, and the protein was centrifuged at a centrifugal force 18800 g of the centrifugal force at 3 ° C. Take 140 μL of the supernatant freeze drying, and analyzed in positive spray ionization and negative electrospray ionization (E...

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Abstract

The invention belongs to the technical field of clinical medical diagnosis and relates to a screening method of early-stage liver cancer diagnosis markers for people with liver cirrhosis and hepatitis. The screening method of the early-stage liver cancer diagnosis markers for people with liver cirrhosis and hepatitis liver cirrhosis and hepatitis comprises the following steps of: S1, acquiring data, specifically, collecting a target plasma sample and detecting metabolites in the plasma sample; S2, constructing a discrimination model used for distinguishing people with liver cirrhosis from patients with the primary liver cancer and distinguishing people with the hepatitis from patients with the primary liver cancer; S3, screening markers; and S4, verifying diagnosis capability. According to the screening method of early-stage liver cancer diagnosis markers for people with liver cirrhosis and hepatitis, a high performance liquid chromatography-mass spectrometry system is utilized to obtain the metabolic profiles of blood plasma of patients with liver cirrhosis, hepatitis and hepatocellular carcinoma, a potential tumor metabolic marker group is found, and a diagnosis model is constructed and is used for assisting early diagnosis of tumors of groups with high risk of liver cancer.

Description

Technical field[0001]The present invention relates to the field of clinical medical diagnosis, in particular, to a screening method of cirrhosis and early liver cancer diagnosis markers in hepatitis population.Background technique[0002]Primary liver cancer is one of the most severe complications of cirrhosis. At present, the 5-year survival rate of liver cancer is generally less than 20%, and the mortality rate accounts for the second place in my country's malignant tumor. Early liver cancer has no specific clinical symptoms, no obvious positive signs or only similar liver hardening body, when there is a significant clinical symptoms and signs, liver cancer is already in the middle and late stage, which greatly affects the healing rate and prognosis of liver cancer. Therefore, early diagnosis and treatment can effectively extend the patient's survival period for high-risk populations. High-risk populations can be deemed: patients with historical history of liver cancer, high virus l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72G01N30/06G01N30/34G01N30/24G01N30/88G01N30/86
CPCG01N30/02G01N30/72G01N30/06G01N30/34G01N30/24G01N30/88G01N30/8696G01N2030/8822G01N2030/884Y02A90/10
Inventor 张顺蔡挺林静张思嘉张沛瑶
Owner MEI HOSPITAL UNIV OF CHINESE ACAD OF SCI
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