Application of all-trans retinoic acid in the preparation of anti-porcine infectious gastroenteritis virus drugs

A technology of all-trans retinoic acid and infectivity, applied in the field of application of all-trans retinoic acid in the preparation of anti-porcine transmissible gastroenteritis virus drugs, can solve the problem of no effective drugs for TGEV infection, and achieve toxicity Less side effects, easy metabolism and absorption effect

Active Publication Date: 2022-08-05
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of this invention is to provide a kind of application of all-trans retinoic acid in the preparation of anti-porcine transmissible gastroenteritis virus medicine, which solves the problem that there is no effective medicine for TGEV infection at present. Replication of transmissible gastroenteritis virus to exert antiviral effect, can be used against porcine transmissible gastroenteritis virus

Method used

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  • Application of all-trans retinoic acid in the preparation of anti-porcine infectious gastroenteritis virus drugs
  • Application of all-trans retinoic acid in the preparation of anti-porcine infectious gastroenteritis virus drugs
  • Application of all-trans retinoic acid in the preparation of anti-porcine infectious gastroenteritis virus drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Determination of cytotoxicity of all-trans retinoic acid on IPEC-J2 cells

[0032] IPEC-J2 cells were counted and diluted to an appropriate density with DMEM-F12 nutrient solution containing 10% fetal bovine serum (FBS), and then the cells were counted at 1×10 4 The concentration of cells / well was added to a 96-well cell culture plate, 100 μL per well, at 37°C, 5% CO 2 After the cells were grown to 70-80% density, IPECs were treated with different concentrations (0, 1, 10, 20, 40, 60, 80, 100, 200 μM / L) of all-trans retinoic acid. - J2 cells (all-trans retinoic acid was solubilized with dimethyl sulfoxide (DMSO), and the same volume of all-trans retinoic acid in DMSO containing different concentrations of all-trans retinoic acid was added to the medium for each treatment group), each Process 8 replicates. After 48 hours of drug action, 10 μL of CCK8 working solution was added to each well, and the culture was continued for 2 hours. Then, the 96-well plate wa...

Embodiment 2

[0034] Example 2 All-trans retinoic acid inhibits porcine transmissible gastroenteritis virus infection on IPEC-J2 cells (flow cytometry and fluorescence quantitative PCR)

[0035] Transfer IPEC-J2 cells to 1 x 10 5 The concentration of 1 / well was seeded in 12-well plate, placed at 37°C, 5% CO 2 Culture in an incubator, when the cells grow to about 80%, discard the old culture medium, and pretreat IPEC-J2 with all-trans retinoic acid at different concentrations (0, 1, 10, 20, 40, 60, 80 μM / L). 12h after the cells were inoculated with 1MOI (multiplicity of infection) of porcine transmissible gastroenteritis virus in IPEC-J2 cells, 37°C, 5% CO 2Incubate in the incubator for 1 h in the presence of all-trans retinoic acid, discard the old culture medium, wash 3 times with PBS, add all-trans retinoic acid corresponding to the previous concentration to each well, place at 37°C, 5% CO 2 After culturing in the incubator for 36 h, the cell samples were collected.

[0036] The cell s...

Embodiment 3

[0038] Example 3 The effect of all-trans retinoic acid on the infection process of porcine transmissible gastroenteritis virus

[0039] 1. The effect of all-trans retinoic acid on preconditioning of IPEC-J2 cells infected with porcine transmissible gastroenteritis virus

[0040] Transfer IPEC-J2 cells to 1 x 10 5 The concentration of 1 / well was seeded in 12-well plate, placed at 37°C, 5% CO 2 Culture in an incubator, when the cells grow to about 80%, discard the old culture medium, pre-treat IPEC-J2 cells with different concentrations (0, 60, 80 μM / L) of all-trans retinoic acid for 12 h, and wash with PBS for 3 and then inoculated IPEC-J2 cells with 1MOI of porcine transmissible gastroenteritis virus at 37°C, 5% CO 2 After 1 h of incubation in the incubator, wash three times with PBS, replace with DMEM-F12 nutrient solution without all-trans retinoic acid, and place at 37°C, 5% CO. 2 After culturing in the incubator for 36 h, the cell samples were collected.

[0041] The c...

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Abstract

The invention discloses the application of all-trans retinoic acid in the preparation of an anti-porcine infectious gastroenteritis virus drug. Acid mainly exerts its antiviral effect by inhibiting the replication of porcine infectious gastroenteritis virus. As the concentration of all-trans retinoic acid increases, the inhibitory effect is more obvious. It is safe and toxic for anti-porcine infectious gastroenteritis virus. It has few side effects, is easily metabolized and absorbed by the animal body, and the animal waste does not pollute the environment.

Description

technical field [0001] The invention relates to an anti-porcine infectious gastroenteritis virus drug, in particular to the application of all-trans retinoic acid in the preparation of an anti-porcine infectious gastroenteritis virus drug. Background technique [0002] Transmissible gastroenteritis ofswine (TGE) is an acute and highly contagious intestinal infectious disease caused by Transmissible gastroenteritis virus ofswine (TGEV). TGEV is an important pathogen of pigs and a member of the family Coronaviridae. It is clinically characterized by vomiting, diarrhea and dehydration. Pigs of different ages and breeds are susceptible to this disease, and piglets within two weeks of age are all susceptible. The mortality rate after infection can reach 100%; with the increase of the age of the infected piglets, the morbidity and mortality of the pig herd decrease, and the clinical symptoms of the infection of pigs over 5 weeks old are only vomiting, watery diarrhea, and weight l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/203A61P31/14
CPCA61K31/203A61P31/14
Inventor 余冰蒲俊宁陈代文伍爱民郑萍田刚虞洁何军毛湘冰
Owner SICHUAN AGRI UNIV
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