Method for cultivating passiflora edulis seedlings

Abstract

The invention discloses a method for cultivating passiflora edulis seedlings. The method for cultivating the passiflora edulis seedlings comprises the following steps: (1) performing seed coat treatment on passiflora edulis seeds, and soaking the passiflora edulis seeds with undiluted sulfuric acid and 80% sulfuric acid correspondingly; (2) calculating the germination rate of seed embryos; (3) taking down sterile leaves, inoculating the sterile leaves into MS culture media containing auxin and cytokinin with different concentration ratios, culturing, and regularly recording and counting differentiation conditions; (4) regularly performing data statistics and recording on the rooting condition of aseptic seedlings; and (5) counting the survival rate. According to the method for cultivating the passiflora edulis seedlings, on the establishment of an in-vitro rapid propagation system of passiflora edulis, the cultivation time is shortened, the cultivation steps are simplified, and a large number of the aseptic seedlings can be provided for the research experiment of the polyploidy of the passiflora edulis established on the in-vitro rapid propagation system.

Description

technical field [0001] The invention relates to the technical field of high-efficiency nursery stock cultivation, in particular to a method for cultivating passion fruit seedlings. Background technique [0002] Passiflora is a typical tropical and subtropical berry. It is native to South America from Brazil to Argentina. It is currently planted in Taiwan, Fujian, Guangdong, Guangxi and Yunnan. The juice of passion fruit has a rich and unique fragrance, and has the flavor of various fruits, so it has the reputation of "king of fruits" and has high nutritional value. Its roots, stems, leaves, flowers, etc. have large medicinal properties. Its seeds can also be obtained by mechanical pressing and solvent extraction to obtain a clear, odorless and high-quality practical oil. [0003] At present, passionflower is mainly used for edible cultivation of purple-fruited passionflower, yellow-fruited passionflower and hybrid varieties. The germplasm resources are relatively scarce and...

AI Technical Summary

Problems solved by technology

[0003] At present, passionflower is mainly used for edible cultivation of purple-fruited passionflower, yellow-fruited passionflower and hybrid varieties. The germplasm resources are relatively scarce and the scope of adaptation is narrow. The main propagation methods are sexual reproduction and asexual reproduction. Sexual reproduction is mainly seed reproduction. Although the seedlings propagated by seeds have stronger root system and strong stress resistance, they have relatively vigorous vegetative growth, less flowering and fruiting, later, greater variation, unstable yield, and fruit. Uneven size, reduced soluble solids content in fruit, poor flavor, weak fragrance and low juice yield, etc., have resulted in a large gap in the market for high-quality seedlings, and the supply exceeds demand, which in turn restricts the passion fruit planting and processing industry development of

Images