A kind of nanocellulose/sodium alginate cryogel and its preparation method and application

A technology of nanocellulose and cryogel, applied in the field of sodium alginate, can solve the problems of poor mechanical properties, slowness, and insufficient chemical crosslinking of cryogel, and achieve good adsorption and purification performance, wide sources, and good biophase capacitive effect

Active Publication Date: 2022-05-10
YUNNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rapid crosslinking leads to aggregation and precipitation of polysaccharides before ice crystal formation, resulting in hydrogels and particles; while slow and insufficient chemical crosslinking leads to poor mechanical properties of cryogels

Method used

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  • A kind of nanocellulose/sodium alginate cryogel and its preparation method and application
  • A kind of nanocellulose/sodium alginate cryogel and its preparation method and application
  • A kind of nanocellulose/sodium alginate cryogel and its preparation method and application

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preparation example Construction

[0031] Specifically, the preparation method of the nanocellulose / sodium alginate cryogel comprises the following steps:

[0032] (1) adding sodium alginate to the nanocellulose suspension with a mass concentration of 0.10 to 0.75 wt %, stirring until it is completely dissolved, to obtain a mixed solution with a mass concentration of sodium alginate of 1.0 to 2.5 wt %;

[0033] (2) adjusting the pH value of the mixed solution obtained in step (1) to 11.0-13.0, adding vinylsulfone until its mass concentration in the mixed solution is 1.00-1.75wt%, stirring, and then placing the mixed solution in -12- Continue the reaction for more than 12h at -20°C;

[0034] (3) After the reaction in step (2) is completed, thaw and wash at room temperature to obtain nanocellulose / sodium alginate cryogel.

[0035] Preferably, wherein the nanocellulose is cellulose nanofibers or cellulose nanocrystals.

[0036] It was confirmed by scanning electron microscopy that the nanocellulose / sodium algina...

Embodiment 1

[0040](1) get 0.5g sodium alginate in 20mL 0.75wt% cellulose nanofibers (sodium alginate is 2.5% of the total solution mass), utilize a high-speed stirrer to stir until completely dissolved;

[0041] (2) Adjust the pH value of the obtained mixed solution to 11.0, add 0.35 mL of vinyl sulfone (vinyl sulfone is 1.75% of the total mass of the solution), stir, pour it into a mold and place it in a -12°C low-temperature test box to react for 12 hours;

[0042] (3) Take out the cryogel, thaw it at room temperature, and wash with a large amount of distilled water to prepare a nanocellulose / sodium alginate cryogel.

[0043] figure 1 The scanning electron microscope image of the nanocellulose / sodium alginate cryogel prepared in this example is shown. Interconnected macropores and lamellar pore walls of tens of micrometers can be observed from the SEM images. In particular, there are many irregular small pores on the walls, and the diameters of these pores are about 3 to 7 μm, which me...

Embodiment 2

[0053] (1) get 0.5g sodium alginate in 20mL 0.10wt% cellulose nanofibers (sodium alginate is 2.5% of the total solution mass), utilize a high-speed stirrer to stir until completely dissolved;

[0054] (2) Adjust the pH value of the obtained mixed solution to a range of 11.0, add 0.35 mL of vinyl sulfone (vinyl sulfone is 1.75% of the total mass of the solution), stir, pour it into a mold and place it in a -12°C low-temperature test box to react for 12 hours;

[0055] (3) Take out the cryogel, thaw it at room temperature, and wash with a large amount of distilled water to prepare a nanocellulose / sodium alginate cryogel.

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Abstract

The invention utilizes freezing-induced oxa-Michael reaction to prepare nanocellulose / sodium alginate cryogel and discloses a nanocellulose / sodium alginate cryogel as well as its preparation method and application, and relates to the technical field of gel preparation . The preparation method is as follows: adding sodium alginate to nano-cellulose suspension, stirring until completely dissolved, adjusting the pH value to 11.0-13.0, adding vinyl sulfone, stirring, and then placing it at -12-20°C to continue the reaction More than 12 hours, after the completion of the reaction, thaw and wash at room temperature to obtain the product. The nanocellulose / sodium alginate cryogel of the present invention not only has excellent mechanical properties, does not need to add any catalyst in the preparation process, and has a simple preparation method, which provides a new way for designing and developing chromatographic media based on natural polysaccharides. At the same time, the preparation method of the material is simple, has good purification performance for lysozyme, and has wide popularization and application value.

Description

technical field [0001] The invention relates to the field of sodium alginate, and more particularly, to a nanocellulose / sodium alginate cryogel and a preparation method and application thereof. Background technique [0002] Currently, high-performance chromatographic media that can effectively separate and adsorb proteins play an important role in the fields of biomedicine and bioseparation. Traditional protein separation and purification methods are mainly carried out by packing chromatographic columns with gel beads medium. However, due to the weak mechanical strength of the gel microbead medium, it usually leads to deformation and packing at high flow rates, which seriously hinders the large-scale application of chromatography techniques. Additionally, gel-bead-packed chromatography media cannot handle unclarified viscous feed streams such as blood, fermentation broth, and egg whites without high back pressure. Therefore, new high-performance chromatographic media with ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/24C08L5/04C08L1/04C08L1/02
CPCC08J3/24C08J2305/04C08J2401/04C08J2401/02C08J2301/04C08J2405/04C08J2301/02
Inventor 张旭锋田乙然张金蒙李昌静包春秀
Owner YUNNAN NORMAL UNIV
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