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Klebsiella mutans sy1 and its application

A technology of Klebsiella and compounds, applied in the application field of microbial degradation of terephthalic acid and phthalic acid, can solve the problems of damage to testicular function, no Klebsiella genus degradation of TPA, etc.

Active Publication Date: 2022-07-19
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

TPA has certain toxicity and has a strong inhibitory effect on microorganisms in water. It can cause bladder stones and bladder cancer and damage testicular function in animals.
There is no report on the degradation of TPA by Klebsiella genus

Method used

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  • Klebsiella mutans sy1 and its application
  • Klebsiella mutans sy1 and its application
  • Klebsiella mutans sy1 and its application

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0026] 1. Experimental materials and methods

[0027] 1.1 Medium:

[0028] LB liquid medium (g / L): yeast extract (5.0), peptone (10.0), NaCl (10.0), the solvent is water, adjusted to pH 7.0, and sterilized at 115°C for 30 minutes;

[0029] LB solid medium is 20g / L agar added to LB liquid medium.

[0030] BSM medium (ie, basal inorganic salt medium, g / L): K 2 HPO 4 ·3H 2 O(1.0), NaCl(1.0), (NH 4 ) 2 SO 4 (0.5), MgSO 4 ·7H 2 O(0.4), CaCl 2 (0.0755), FeCl 3 ·6H 2 O(0.0143), the solvent is water, adjusted to pH 7.5, sterilized at 115°C for 30min;

[0031] BSM solid medium was supplemented with 20 g / L agar in BSM medium.

[0032] TPA liquid medium: add terephthalic acid (TPA) (dissolved in DMSO, the concentration of the stock solution is 250 mg / mL) in the BSM medium, so that the final concentration of TPA in the solution is 250 mg / L.

[0033] TPA solid medium: add 20g / L agar to TPA liquid medium.

[0034] 1.2 Screening of TPA-degrading bacteria

[0035] Enrichment c...

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Abstract

The invention discloses a Klebsiella mutans SY1 and an application thereof. The application comprises the following steps: inoculating the Klebsiella mutans SY1 into a BSM medium containing phenyl dicarboxylic acid compounds, at pH 4. The degradation reaction is carried out under the conditions of 0-9.0, 20-40° C., and 100-200 rpm, and after the reaction is complete, the degradation of the phenyldicarboxylic acid compounds is realized; in the present invention, a single strain can be used to effectively degrade TPA, and at the same time, it can also degrade o-phthalic acid. Important phthalic acid compounds such as dicarboxylic acid or bishydroxyethyl terephthalate can be used for purification of purified terephthalic acid production wastewater, chemical fiber wastewater, etc.

Description

(1) Technical field [0001] The present invention relates to a strain with terephthalic acid (TPA) or phthalic acid (PA) degrading ability - Klebsiella variicola SY1, and its microbial degradation of terephthalic acid (Klebsiella variicola) SY1 TPA) and phthalic acid (PA). (2) Background technology [0002] Terephthalic acid (terephthalic, TPA) is the main raw material for the production of polyethylene terephthalate (Polyethleneterephthalate, PET), and is also an important and major degradation product in the process of PET degradation. In addition, TPA is also used in the production of coatings, adhesives, plasticizers and dyes and other industries, and is an important industrial raw material. According to reports, the global production of TPA has reached 1.25 million tons / year. The production of every ton of TPA will generate 3 to 4 m 3 wastewater containing 1500mg / L of TPA. TPA has certain toxicity, has a strong inhibitory effect on microorganisms in water, and can ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/22C02F101/34
CPCC12N1/20C02F3/34C02F2101/34
Inventor 邱乐泉吴石金尹辛格钟卫鸿李骏李彤彤
Owner ZHEJIANG UNIV OF TECH