Preparation method and application of green fluorescent protein labeled Chinese rose fusarium

Abstract

The invention belongs to the field of phytopathology, and provides a preparation method and application of green fluorescent protein labeled Chinese rose fusarium, a Chinese rose fusarium strain YJ1 is used as a transformant, and a genetic transformation method of PEG-mediated protoplast is utilized to mark the Chinese rose fusarium strain YJ1 with a green fluorescent protein gene, so that the green fluorescent protein gene labeled Chinese rose fusarium strain YJ1 is obtained, the Chinese rose fusarium strain YJ1-GFP marked with the green fluorescent protein can be used for construction and screening of a mutant library of the Chinese rose fusarium strain, quantitative identification of host resistance, a response mechanism of a host to infection of the Chinese rose fusarium strain and research on infection and pathogenesis of the Chinese rose fusarium strain. The preparation method of the green fluorescent protein labeled Chinese rose fusarium provided by the invention is quick and convenient, simplified in steps and short in time consumption, and the Chinese rose fusarium strain YJ1-GFP labeled by the green fluorescent protein can be directly picked from a flat plate.

Description

technical field [0001] The invention belongs to the technical field of plant pathology, and in particular relates to a preparation method and application of green fluorescent protein-labeled Fusarium rosea. Background technique [0002] Rose Fusarium wilt is a vascular systemic fungal disease caused by Fusarium rosicola sp.nov., which can infect all parts of rose rhizomes and leaves. In the early stage of disease symptoms, the leaves turn green and yellow, and gradually fall off, eventually causing The whole rose plant dies. Fusarium rosea has a wide range of hosts and can harm many flowers such as roses, roses, roses, and cherry blossoms in the Rosaceae family. Winter and spring solar greenhouses and greenhouse cultivation in early spring and autumn all occur, and the damage is extremely serious. To obtain disease-resistant varieties, we must first understand the molecular genetic basis and pathogenic mechanism of Fusarium rosea. At present, some progress has been made in...

AI Technical Summary

Problems solved by technology

However, at present, there have been reports at home and abroad using different genetic transformation methods to transform green fluorescent protein-labeled (GFP) fungi, but the reported GFP-labeled fungal strain methods either do not involve specific label-related details, or the marker transformation steps are cumbersome and inefficient , the fluorescence intensity is unstable

At present, there is no report on the GFP-labeled strain and method of Fusarium rosea, the pathogen of Fusarium rosea, which has become a bottleneck in the research on the pathogenic process and mechanism of Fusarium rosea infection

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