Method for producing biodiesel by using red tide algae and algicidal bacteria

A technology of biodiesel and red tide algae, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of high cost of cell crushing and mechanical crushing, and achieve the goals of simplifying the process of splitting algae, saving production costs, and saving energy Effect

Pending Publication Date: 2021-06-25
XIAMEN UNIV
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Problems solved by technology

[0006] The purpose of the present invention is to solve the problems of high cost of cell crushing and mechanical crushing in the prior art, and provide a method for p

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  • Method for producing biodiesel by using red tide algae and algicidal bacteria
  • Method for producing biodiesel by using red tide algae and algicidal bacteria
  • Method for producing biodiesel by using red tide algae and algicidal bacteria

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Embodiment Construction

[0031] The following embodiments will further illustrate the present invention in conjunction with the accompanying drawings.

[0032] 1. Materials and methods

[0033] 1.1 algae species and cultivation: the algae species used in the embodiment of the present invention is Prorocentrum donghaiense (Prorocentrum donghaiense), which is preserved in the State Key Laboratory of Offshore Marine Environmental Science of Xiamen University (strain number: 364, separated from the Yangtze Estuary of the East China Sea). Buy. Cultivate in f / 2 liquid medium with an inoculum of 4%, and grow at room temperature 20-27°C and light intensity 50-200μmoL photons m -2 the s -1 Cultured under light and dark (12h / 12h) conditions.

[0034] Table 1 Main components of f / 2 medium

[0035]

[0036] 1.2 Algae-dissolving strains and cultivation:

[0037] The algicidal strain Paracoccus sp.Y42 was isolated from the Zhangzhou Mangrove National Nature Reserve and was preserved in the Guangdong Microbi...

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Abstract

The invention discloses a method for producing biodiesel by using red tide algae and algicidal bacteria, and relates to biodiesel. The method comprises the following steps: inoculating an f/2 culture medium with red tide algae cells, and standing and culturing in an illumination culture room; culturing algicidal bacteria Y42 in a liquid culture medium to obtain sterile supernate; adding the sterile supernate into a prorocentrum donghaiense culture solution for continuous culture, oxidizing fatty acid, and lysing red tide algae cells; repeatedly extracting the lysedalgae liquid by adopting a rotary evaporation method, and drying to obtain a crude fat extract; and performing methyl esterification on the grease to obtain the biodiesel production raw material. The supernatant of the algae-lysing strain Y42 is used for lysing algae cells, so that the algae-lysing process of downstream extraction is simplified, the production cost is saved, the grease composition can be changed, the grease unsaturation degree of the algae cells is effectively reduced, and the grease IV < 120, CN > 47, and the grease composition is suitable for biodiesel production. And 90% or more of algae cells can be lysed after the algae killing bacteria are treated for 72 hours, so that the development of biodiesel is facilitated, and harm is turned into treasure.

Description

technical field [0001] The invention relates to biodiesel, in particular to a method for producing biodiesel by utilizing red tide algae and alginolytic bacteria. Background technique [0002] Biomass energy is a renewable energy source with a wide range of sources. Its development and utilization will not only help alleviate the crisis brought by the depletion of fossil fuels to the global economic development, but also avoid environmental pollution. In recent years, the advantages of algae as a new type of energy raw material are obvious and have attracted widespread attention. Algae are aquatic plants that do not occupy arable land or animal husbandry area; the growth environment is simple, and the nutrients required are mainly sunlight, water and CO 2 , fixed CO 2 It can be converted into oil or starch, thereby alleviating the greenhouse effect and benefiting the environment. Algae has a short growth cycle, fast reproduction speed, very high yield, no roots, stems, le...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12N1/12C12N1/20C12R1/89C12R1/01
CPCC12P7/649C12N1/12C12N1/20Y02E50/10
Inventor 徐虹范永香张福星桂嘉丽
Owner XIAMEN UNIV
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