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Microcapsule fungicide, preparation method and application thereof

A technology for microcapsules and inoculants, applied in the field of microcapsule inoculants and their preparation, can solve the problems of loss of viable counts of microorganisms, inconvenient transportation of liquid inoculants, and environmental pollution.

Inactive Publication Date: 2021-06-29
SHENZHEN BATIAN ECOTYPIC ENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there have been many microbial agents used in agricultural production, most of these microbial agents are in the form of bacterial powder or granules, which cause great damage to the number of microorganisms in the production process, and are not easy to preserve and store for a long time. soil colonization
Moreover, existing microbial inoculum products mainly include liquid inoculum and solid inoculum. Liquid inoculum is inconvenient to transport and has a short shelf life; solid inoculum includes powder and granule, which has a longer shelf life than liquid inoculum. However, the number of viable microorganisms is lost during the production process, and the waste gas and wastewater discharged from the equipment will pollute the environment, and the final application will be affected by many factors in the soil, making it difficult to play the actual role of microorganisms

Method used

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  • Microcapsule fungicide, preparation method and application thereof
  • Microcapsule fungicide, preparation method and application thereof
  • Microcapsule fungicide, preparation method and application thereof

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preparation example Construction

[0046] According to one aspect of the present invention, a kind of preparation method of above-mentioned microcapsule bacterial agent, described preparation method comprises the following steps:

[0047] (a), providing a functional microbial suspension, and then mixing the suspension with a sodium alginate solution to obtain solution A;

[0048] (b), solution A is added dropwise in the calcium chloride solution to form the inner core as a functional microorganism, and the outer layer is solidified particles of calcium alginate, then the solidified particles are added to the chitosan solution to coat the chitosan layer, Prepared microcapsule bacterial agent.

[0049] The preparation method of the microcapsule bacterial agent provided by the present invention, the preparation method first provides a functional microbial suspension, then mixes the bacterial suspension with a sodium alginate solution to obtain a solution A; then adds the solution A dropwise to the chlorinated In ...

Embodiment 1

[0068] A kind of functional microbial bacterial suspension, described preparation method comprises the following steps:

[0069] (1) The Bacillus was isolated from the soil of the greenhouse. After identification, the strain was Bacillus tequilensis with the preservation number CGMCC NO.20316. The activation medium is LB liquid medium, and the medium formula is: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L. The culture conditions were 37° C., 150 rpm, and cultured for 48 hours.

[0070] (2), the thalline collection after step (1) is cultivated is to collect after centrifuging 15min at 4000rpm, then add sterile water, blow and suck to make a bacterium suspension, so that the concentration of the bacterium suspension is 10 8 cfu / mL to prepare a functional microbial suspension.

Embodiment 2

[0072] figure 1 The physical picture of the solidified particles after coating the calcium alginate layer with functional microorganisms prepared in this example;

[0073] figure 2 It is the physical picture of the microcapsule bacterial agent after the coating chitosan layer that the present embodiment makes.

[0074] like figure 1 and figure 2 Shown, a kind of preparation method of microcapsule bacterial agent, described preparation method comprises the following steps:

[0075] (a), get the functional microbial bacterial suspension that embodiment 1 makes and the sodium alginate solution that the mass concentration of sterilization is 2% and mix uniformly, obtain solution A; Functional microbial bacterial suspension in described solution A and The volume ratio of sodium alginate solution is 0.6:1;

[0076] Then, when the stirring speed is 400rpm, use a sterile syringe with a diameter of 0.45nm to drop the obtained solution A into a calcium chloride solution with a ma...

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Abstract

The invention provides a microcapsule fungicide, a preparation method and application thereof, and relates to the technical field of agricultural microbial agents. The microcapsule fungicide comprises a core material and a wall material, wherein the core material is functional microorganisms, the wall material is calcium alginate and chitosan, and the calcium alginate gel and the chitosan are finally compounded and coated on the outer layer of the functional microorganism. According to the invention, the microcapsule fungicide with the structure has the advantages of high density, high microorganism survival rate and stable storage, can effectively adjust the slow release and colonization effects of thalli in soil and effectively prolong the shelf life of the microbial fungicide.

Description

technical field [0001] The invention relates to the technical field of agricultural microbial inoculum, in particular to a microcapsule inoculum and its preparation method and application. Background technique [0002] As a green and safe control method, biological control has certain advantages, but there are also some shortcomings. The application of functional microorganisms is mostly restricted by various ecological factors such as the soil environment, and it is difficult to be well used in different soils. The completion of colonization makes the microbial agent unable to play a real role in actual use. At the same time, there are still bottleneck problems in the preparation process of agricultural microbial preparations, such as relatively low viable bacteria content, backward post-processing and processing technology. [0003] At present, after long-term use of chemical fertilizers, soil compaction and acidification have been extremely serious. As a green and healthy...

Claims

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Application Information

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IPC IPC(8): A01N63/22A01N25/28A01P3/00A01P1/00A01P21/00C09K17/14C09K17/32C09K101/00
CPCA01N63/22A01N25/28C09K17/14C09K17/32C09K2101/00
Inventor 张志鹏段继贤邓祖科华建青吴书凤王宗抗彭启超黄德龙
Owner SHENZHEN BATIAN ECOTYPIC ENG
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