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Four-year-old oil peony rhizosphere pseudomonas Jergeri FD4-8 and application thereof

A technology of Pseudomonas, peony root, applied in the field of microbiology

Inactive Publication Date: 2021-07-06
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on the use of this strain for the degradation of organic phosphorus

Method used

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  • Four-year-old oil peony rhizosphere pseudomonas Jergeri FD4-8 and application thereof
  • Four-year-old oil peony rhizosphere pseudomonas Jergeri FD4-8 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Example 1 Isolation and purification of a four-year-old oil-used Pseudomonas jayzii FD4-8 from the peony rhizosphere

[0081] Firstly, remove the visible debris on the rhizosphere soil surface of the four-year oil peony planted in the experimental farm of Kaiyuan Campus of Henan University of Science and Technology in Luoyang City, Henan Province. According to the five-point sampling method, the rhizosphere soil was collected with a soil drill at a distance of 10 cm from the plant. Put it into a sterile bag and store it in an ice box. After arriving at the laboratory, use a brush to brush off the soil attached to the root system, weigh 5g of fresh soil samples in the ultra-clean workbench into a sterile centrifuge tube, dilute to 50mL with sterile water, shake for 20min, and press 10 The dilutions were carried out sequentially, and a pipette was used to draw 10 -6 ~10 -4 Each 0.1mL sample of the three dilutions was evenly spread on the organophosphate solid medium, an...

Embodiment 2 4

[0083] Example 2 Identification of Pseudomonas Jaysoni FD4-8 in the rhizosphere of peony for four-year oil production

[0084] Bacterial genomic DNA was extracted using the AxyPrep Bacterial Genomic DNA Miniprep Kit. Genomic DNA was amplified using bacterial universal primers 27F: 5'-AGAGTTTGATCCTGGCTCAG-3' and 1492R: 5'-CTACGGCTACCTTGTTACGA-3' to obtain PCR products. PCR products were purified with AxyPrep DNA. The purified PCR products were bidirectionally sequenced using the ABI3730-XL sequencer at Shanghai Passino Biotechnology Co., Ltd. After using ChromasPro software to truncate the invalid sequence, use BioEdit software to splice the double-ended effective sequence, and then use the Blast program in the NCBI database to compare the effective sequence with the reference sequence in the NCBI database to obtain a sequence similar to the strain to be tested The most sexual strain is Pseudomonasjessenii, with a homology of 99%.

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Abstract

The invention relates to the technical field of microorganisms, in particular to a four-year-old oil peony rhizosphere pseudomonas Jergeri FD4-8 and application of the four-year-old oil peony rhizosphere pseudomonas Jergeri FD4-8. According to the present invention, the application of the strain in the organophosphorus degradation test results show that the content of the rapidly available phosphorus activated from the calcium phytate by the strain is 4.96 mg / L, and the activation rate of the calcium phytate reaches 59.66%. Therefore, the pseudomonas jersei can also be used for degrading organic phosphorus, and the degradation effect is very good.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a four-year oil-growing Pseudomonas japonica FD4-8 and its application. Background technique [0002] Organophosphate-decomposing bacteria refer to bacteria that can decompose organic phosphorus compounds. There are various types of bacteria, and different bacteria have different phosphorus-dissolving mechanisms. The mechanism of microbial degradation of organophosphate is generally believed to be related to the enzymes secreted. Organophosphate-degrading bacteria degrade organophosphate by secreting extracellular phosphatase. When phosphorus becomes the main limiting factor for microbial growth, microorganisms will release extracellular phosphatase to hydrolyze organic phosphorus into available phosphorus. At the same time, microbial intracellular alkaline phosphatase will be induced to synthesize and accumulate continuously. [0003] Pseudomonas jessenii belongs to the genus ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/14C12R1/38C09K101/00
CPCC12N1/20C09K17/14C09K2101/00
Inventor 侯小改郭丽丽李昱莹宋鹏刘曙光牛童非
Owner HENAN UNIV OF SCI & TECH
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