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Narrow-leaf peony rhizosphere Azotobacter chroococcum PP30 strain and application

A technology of nitrogen-fixing bacteria brown balls and peony roots, applied in the field of microorganisms

Inactive Publication Date: 2021-07-06
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there has been no report on the application of phloeosphaeroids in degrading organic phosphorus.

Method used

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  • Narrow-leaf peony rhizosphere Azotobacter chroococcum PP30 strain and application
  • Narrow-leaf peony rhizosphere Azotobacter chroococcum PP30 strain and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 1

[0079] Example 1 Isolation and purification of a nitrogen-fixing bacteria Pheococcus PP30 in the rhizosphere of Paeonia angustifolia

[0080] First remove the visible debris on the surface of the rhizosphere soil of wild peony and peony angustifolia from the Gansu Provincial Agricultural Technology Extension Station. According to the five-point sampling method, use a soil drill to collect the rhizosphere soil at a distance of 10cm from the plant, and put it into a sterile bag , stored in an ice box. After arriving at the laboratory, use a brush to brush off the soil attached to the root system, weigh 5g of fresh soil samples in the ultra-clean workbench into a sterile centrifuge tube, dilute to 50mL with sterile water, shake for 20min, and press 10 The dilutions were carried out sequentially, and a pipette was used to draw 10 -6 ~10 -4 Each 0.1mL sample of the three dilutions was evenly spread on the organophosphate solid medium, and each gradient was repeated three times. ...

Embodiment 2

[0082] Example 2 Identification of Rhizosphere Pheococcus PP30 of Paeonia angustifolia

[0083] Bacterial genomic DNA was extracted using the AxyPrep Bacterial Genomic DNA Miniprep Kit. Genomic DNA was amplified using bacterial universal primers 27F: 5'-AGAGTTTGATCCTGGCTCAG-3' and 1492R: 5'-CTACGGCTACCTTGTTACGA-3' to obtain PCR products. PCR products were purified with AxyPrep DNA. The purified PCR products were bidirectionally sequenced using the ABI3730-XL sequencer at Shanghai Passino Biotechnology Co., Ltd. After using ChromasPro software to truncate the invalid sequence, use BioEdit software to splice the double-ended effective sequence, and then use the Blast program in the NCBI database to compare the effective sequence with the reference sequence in the NCBI database to obtain a sequence similar to the strain to be tested The strain with the highest sex is Azotobacter chroococcum, with a homology of 99.59%.

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PUM

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Abstract

The invention relates to the technical field of microbes and particularly relates to a narrow-leaf peony rhizosphere Azotobacter chroococcum PP30 strain and application. Through applying the strain disclosed by the application to tests of degradation of organophosphorus, it is discovered that the content of rapidly available phosphorus activated from calcium phytate by the strain is 4.44mg / L, and the activation rate of the calcium phytate reaches 76.74%. Obviously, the Azotobacter chroococcum not only can exert a biological nitrogen fixation action, but also can be applied to the degradation of the organophosphorus, and the degradation effect is very good.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a nitrogen-fixing bacterium Pheosphaerus PP30 and its application. Background technique [0002] Azotobacter chroococcum belongs to the order Pseudomonas, the family Pseudomonas, and the genus Azotobacter chroococcum. The characteristics are: the cells are large, club-shaped or rod-shaped, the size of the bacteria is 2.0-3.0×3.0-6.0 μm, and they are often arranged in pairs or octaples; the bacteria have capsules, perinatal flagella, and can move. Gram negative. It grows well on nitrogen-free medium, the lawn is sticky and pasty, the lawn is transparent to milky white in young culture, and it is often brown in culture. Commonly used in (1) biological nitrogen fixation; (2) as bacterial fertilizer (mostly combined nitrogen fixation), to increase crop yield, improve crop quality, improve crop stress resistance, and reduce dependence on chemical fertilizers; (3) Determination of n...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/14C12R1/065C09K101/00
CPCC12N1/20C09K17/14C09K2101/00
Inventor 郭丽丽侯小改王菲宋程威范丙友罗亚桑
Owner HENAN UNIV OF SCI & TECH
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