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Composite microecological agent for aquaculture and scenery water body and its preparation method

A compound micro-ecology and aquaculture technology, which is applied in the field of compound micro-ecology preparations for aquaculture and landscape water bodies, can solve the problems of not considering the biological safety of aquaculture, the narrow range of microbial strains adapting to the temperature, and the unstable product quality and performance and other problems, to achieve the effect of improving the number of effective viable bacteria, the types of bacteria, and the number of bacteria

Inactive Publication Date: 2009-03-11
南京市水产科学研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many limitations in existing products: the temperature range of microbial strains is narrow; the bacteria are too small, the flocculation properties are not good, and it is not easy to collect; the number of strains is relatively single, and the adaptability to various water quality types is not strong. The scope of application is not wide enough; the safety of aquaculture organisms is not considered, and the products produced by extensive solid-state fermentation and liquid fermentation have problems such as unstable quality and performance, and a small number of effective viable bacteria

Method used

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  • Composite microecological agent for aquaculture and scenery water body and its preparation method
  • Composite microecological agent for aquaculture and scenery water body and its preparation method
  • Composite microecological agent for aquaculture and scenery water body and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: the preparation of compound probiotics fermentation medium

[0034] The fermentation medium of 4 strains shall be prepared according to the following requirements (unit: g / L):

[0035] Candida aquatic: molasses 10, glucose 10, ammonium chloride 1, yeast extract 0.2, potassium dihydrogen phosphate 0.6, dipotassium hydrogen phosphate 0.7, magnesium sulfate 0.4, sodium chloride 0.5, ferrous sulfate 4×10 -3 , water 1L.

[0036] Spherical yeast: molasses 10, glucose 10, ammonium chloride 1.5, yeast extract 0.3, potassium dihydrogen phosphate 0.5, dipotassium hydrogen phosphate 0.7, magnesium sulfate 0.5, sodium chloride 0.5, ferrous sulfate 5×10 -3 , water 1L.

[0037] Bacillus subtilis: molasses 10, peptone 2, glucose 5, ammonium chloride 1, yeast extract 0.35, potassium dihydrogen phosphate 0.4, dipotassium hydrogen phosphate 0.7, magnesium sulfate 0.5, sodium chloride 0.5, ferrous sulfate 4.5×10 -3 , water 1L.

[0038] Pseudomonas stutzeri: molasses 10, ...

Embodiment 2

[0040] Embodiment 2: the preparation of compound probiotics seed culture medium

[0041] The big shake flask seed culture medium of 4 strains was prepared according to the following requirements (unit: g / L):

[0042] Candida aquatic: molasses 10, glucose 10, ammonium chloride 1, yeast extract 0.2, potassium dihydrogen phosphate 0.6, dipotassium hydrogen phosphate 0.7, magnesium sulfate 0.4, sodium chloride 0.5, ferrous sulfate 4×10 -3 , water 1L.

[0043] Spherical yeast: molasses 10, glucose 10, ammonium chloride 1.5, yeast extract 0.3, potassium dihydrogen phosphate 0.5, dipotassium hydrogen phosphate 0.7, magnesium sulfate 0.5, sodium chloride 0.5, ferrous sulfate 5×10 -3 , water 1L.

[0044] Bacillus subtilis: molasses 10, peptone 2, glucose 5, ammonium chloride 1, yeast extract 0.35, potassium dihydrogen phosphate 0.4, dipotassium hydrogen phosphate 0.7, magnesium sulfate 0.5, sodium chloride 0.5, ferrous sulfate 4.5×10 -3 , water 1L.

[0045] Pseudomonas stutzeri: mola...

Embodiment 3

[0047] Example 3: Research on production and fermentation process parameters of compound probiotics

[0048] Four different strains of microorganisms were cultured and fermented under different inoculum size, ventilation rate, initial pH value, culture temperature and culture time, and colony counts were performed on the fermentation products to screen the best fermentation conditions. The dynamic change of the fermentation substrate and the change of the yield of the fermentation product were analyzed respectively at 6h, 9h, 12h, 15h, 18h and 24h after fermentation to understand the real-time fermentation situation. On the basis of the above experiments, the specific process conditions and requirements of two different production processes of batch fermentation and continuous fermentation were established, and the production methods under different requirements were proposed. The test results are shown in Table 1 and Table 2.

[0049] Table 1 The growth of microorganisms unde...

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Abstract

The invention discloses a compound micro-ecological agent and preparing method of aquaculture and landscape water body, which is allocated by aquatic candida, ball-shaped yeast, bacillus subtilis and shipseudomonas with quality rate at 0.5-1.7:0.0-1.5:1.0-3.0:1.0-2.5. The agent can decompose eutrophication material in the aquaculture and landscape water body, which inhibits blue-green algae effectively.

Description

technical field [0001] The invention relates to a composite micro-ecological preparation for aquaculture and landscape water bodies, belonging to the field of aquatic microbiology and ecological products and their manufacture. Background technique [0002] Generally, high-density and intensive methods are adopted in the breeding process of aquatic products, which causes many harmful substances to accumulate in the water body, thereby directly or indirectly harming the cultured organisms in the water body. Micro-ecological control technology has been increasingly valued by people due to its many advantages. There are many products for sale. However, there are many limitations in existing products: the temperature range of microbial strains is narrow; the bacteria are too small, the flocculation properties are not good, and it is not easy to collect; the number of strains is relatively single, and the adaptability to various water quality types is not strong. The scope of app...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12N1/20C02F3/34C12R1/72C12R1/125C12R1/38
Inventor 周国勤吴伟茆建强陈树桥
Owner 南京市水产科学研究所
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