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Buffer solution and application thereof in central nervous system specific protein detection kit

A technology of central nervous system and buffer solution, applied in the field of immunodetection, can solve the problems of poor sensitivity of brain injury detection kit, poor sensitivity of kit, complicated preparation process, etc., and achieves high precision, sensitivity and specificity, high sensitivity, The effect of short reaction time

Active Publication Date: 2021-07-09
SOPHONIX CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, the use of kits generally has poor sensitivity, or the preparation process is complicated, the detection time is long, and the cost is high.
The invention aims to solve the problems of poor sensitivity and slow detection time of the brain injury detection kit existing in the prior art

Method used

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  • Buffer solution and application thereof in central nervous system specific protein detection kit
  • Buffer solution and application thereof in central nervous system specific protein detection kit
  • Buffer solution and application thereof in central nervous system specific protein detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] A buffer solution, in parts by weight, the buffer solution includes the following components: HEPES 3.0g, sodium chloride 9g, potassium chloride 2g, magnesium sulfate 5g, BSA 27g, trehalose 40g, glycine 18g, Casein 15g , Glycerin 100ml.

[0067] The preparation method of described buffer solution, comprises the following steps:

[0068] (1) Weigh HEPES, NaCl, KCl, MgSO 4 , trehalose, bovine serum albumin, glycine, casein, and glycerin were added to purified water and stirred until completely dissolved, adjusted to pH 6.5, and set to 1000ml;

[0069] (2) Filter with a 0.22 μm filter membrane to obtain the buffer solution. (Denoted as buffer S1)

Embodiment 2

[0071] A buffer solution, in parts by weight, the buffer solution includes the following components: HEPES 1.0g, sodium chloride 5g, potassium chloride 0.5g, magnesium sulfate 10g, BSA 30g, trehalose 10g, glycine 20g, Casein 0.5g, glycerin 20ml.

[0072] The preparation method of described buffer solution, comprises the following steps:

[0073] (1) Weigh HEPES, NaCl, KCl, MgSO 4 , trehalose, bovine serum albumin, glycine, casein, and glycerin were added to purified water and stirred until completely dissolved, adjusted to pH 8.2, and set to 1000ml;

[0074] (2) Filter with a 0.22 μm filter membrane to obtain the buffer solution. (Denoted as buffer S2)

Embodiment 3

[0076] A buffer solution, in parts by weight, the buffer solution includes the following components: HEPES 10g, sodium chloride 10g, potassium chloride 5g, magnesium sulfate 0.1g, BSA 4.0g, trehalose 100g, glycine 4.0g, Casein 25g, glycerin 200ml.

[0077] The preparation method of described buffer solution, comprises the following steps:

[0078] (1) Weigh HEPES, NaCl, KCl, MgSO 4 , trehalose, bovine serum albumin, glycine, casein, and glycerin were added to purified water and stirred until completely dissolved, adjusted to pH 7.0, and set to 1000ml;

[0079] (2) Filter with a 0.22 μm filter membrane to obtain the buffer solution. (Denoted as buffer S3)

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Abstract

The invention belongs to the technical field of immunodetection, and particularly relates to a buffer solution and application thereof in a central nervous system specific protein detection kit. The buffer solution is prepared from the following components: 4-hydroxyethyl piperazine ethanesulfonic acid (HEPES), sodium chloride (NaCl), potassium chloride (KCl), magnesium sulfate (MgSO4), bovine serum albumin (BSA), trehalose, glycine, casein (Casein) and glycerol. The buffer solution can be applied to a central nervous specific protein detection kit, is used for detecting brain injury, has relatively high sensitivity, and can complete detection in a relatively short time.

Description

technical field [0001] The invention belongs to the technical field of immunoassay, and in particular relates to a buffer solution and its application in a central nervous system-specific protein detection kit. Background technique [0002] Accurate evaluation of the severity and prognosis of central nervous system damage after traumatic brain injury (traumatic brain injury, TBI) is very important for clinical treatment. At present, there is no more accurate means to evaluate the severity of TBI, and it is difficult to judge the prognosis. Cognitive dysfunction, motor dysfunction, sensory dysfunction, emotional and emotional disorders caused by central nervous system damage seriously affect the quality of life of patients. [0003] S100-β is an acidic calcium-binding protein with a molecular weight of 21KD, which is mainly produced by astrocytes, forms disulfide bonds through cysteine ​​residues, and exists in the central nervous system in a large amount in the active form ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/58G01N33/543G01N33/535G01N33/532
CPCG01N33/6893G01N33/581G01N33/54326G01N33/535G01N33/532G01N2800/2814G01N2800/2835G01N2333/4727
Inventor 王法龙刘聪魏宏娟李博飞
Owner SOPHONIX CO LTD
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