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New application of aloe-emodin as anti-mast cell activator

A technology of mast cells and activators, applied in the field of medicine

Active Publication Date: 2021-07-16
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing pharmacological studies have shown that aloe-emodin has various activities such as anti-inflammatory, antibacterial, anti-tumor, and hypolipidemic, and can also prevent and treat myocardial ischemia and arrhythmia, and improve non-alcoholic fatty liver. No prior art was found disclosing aloe-emodin as an anti-mast cell activator

Method used

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  • New application of aloe-emodin as anti-mast cell activator
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  • New application of aloe-emodin as anti-mast cell activator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Effect of aloe-emodin on the mortality of Compound48 / 80-induced anaphylactic shock mice.

[0016] (1) Experimental materials:

[0017] Aloe-emodin (Adamas Reagent Co., Ltd., purity > 99%), ketotifen fumarate (CAS No.: 34580-14-8, M.W.425.5, TCI Shanghai Chemical Industry Development Co., Ltd., purity > 98%) %), sodium cromoglycate (CAS No.: 15826-37-6, M.W.512.33, Shanghai Yuanye Biotechnology Co., Ltd., purity > 98%), Compound48 / 80 (Sigma-Aldrich, Cat No.C2313); SPF male Balb / c mice (18g-20g, Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.); sterile normal saline, disposable sterile syringes.

[0018] (2) Experimental method:

[0019] Mice are randomly divided into normal control group, model group, positive drug ketotifen group, positive drug sodium cromoglycate group, aloe-emodin large, medium and small three dosage groups (12.5mg / kg, 25mg / kg and 50mg / kg) according to body weight. kg), 15 in each group. During the experiment, the mice in ...

Embodiment 2

[0025] Example 2 Effect of aloe-emodin on Compound48 / 80-induced release of β-hexosaminidase from human mast cell line LAD2.

[0026] (1) Experimental materials:

[0027] Aloe-emodin (Adamas Reagent Co., Ltd., purity > 99%), ketotifen fumarate (CAS No.: 34580-14-8, M.W.425.5, TCI Shanghai Chemical Industry Development Co., Ltd., purity > 98%) %), sodium cromoglycate (CAS No.: 15826-37-6, M.W.512.33, Shanghai Yuanye Biotechnology Co., Ltd., purity > 98%), Compound48 / 80 (Sigma-Aldrich, Cat No.C2313), 4-methyl Umbelliferyl N-acetyl-β-D-glucosamine (Sigma-Aldrich, Cat No. 69585); human mast cell line LAD2 was obtained from NIH in the United States.

[0028] (2) Experimental method:

[0029] The LAD2 cells in the logarithmic growth phase were divided into 6×10 4 Each well was evenly added in a 96-well plate, and different concentrations of test substances (50 μM positive drug or 5 μM-20 μM aloe-emodin) were added to the administration wells, and an equal volume of Hank’s solution...

Embodiment 3

[0032] Example 3 Effect of aloe-emodin on Compound48 / 80-induced release of β-hexosaminidase from primary mouse peritoneal mast cells.

[0033] (1) Experimental materials:

[0034]Aloe-emodin (Adamas Reagent Co., Ltd., purity > 99%), ketotifen fumarate (CAS No.: 34580-14-8, M.W.425.5, TCI Shanghai Chemical Industry Development Co., Ltd., purity > 98%) %), sodium cromoglycate (CAS No.: 15826-37-6, M.W.512.33, Shanghai Yuanye Biotechnology Co., Ltd., purity > 98%), Compound48 / 80 (Sigma-Aldrich, Cat No.C2313), 4-methyl Umbelliferyl N-acetyl-β-D-glucosamine (Sigma-Aldrich, Cat No.69585); SPF male Balb / c mice (18g-20g, Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. ); sterile saline, disposable sterile syringe.

[0035] (2) Experimental method:

[0036] a) Obtaining primary mouse peritoneal mast cells:

[0037] Mice were sacrificed by decapitation, and the peritoneal cavity of the mice was washed with ice-cold PBS solution. After repeated gentle rubbing for 3-5 m...

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Abstract

The invention belongs to the technical field of medicines, and discloses a new application of aloe-emodin (the English name is aloe-emodin; and the CAS number is 481-72-1) as an anti-mast cell activator. In-vitro pharmacodynamic experiment results show that the aloe-emodin can remarkably reduce release of mast cell sensitizers and stabilize mast cell membranes on the premise of not influencing cell activity (non-toxicity), and has a strong and remarkable mast cell activation resisting effect. Animal experiment results show that the aloe-emodin can significantly improve the survival rate of allergic shock mice and has an obvious protective effect. Therefore, the invention provides the new application of the aloe-emodin as the anti-mast cell activator, and the aloe-emodin can be used for preparing medicines, health-care products, foods or cosmetics for diseases related to mast cell activation.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the use of aloe-emodin as an anti-mast cell activator. Background technique [0002] Mast cells are an important class of immune cells that originate from bone marrow CD13 + 34 + CD117 + hematopoietic progenitor cells. Mast cells can differentiate under the induction of stem cell growth factors or other cytokines that regulate development, and are widely distributed in the skin, mucous membranes, airways, etc., to respond to allergens in the environment in a timely manner. When the body is subjected to different external stimuli, it will cause the activation of mast cells, synthesize and release a variety of biologically active media and cytokines in the granules, such as β-hexosaminidase, histamine, 5-HT, tryptase, white Trienes, prostaglandins, etc., which in turn lead to a series of pathological changes. Thus, the activation of mast cells is a prerequisite fo...

Claims

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Application Information

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IPC IPC(8): A61K31/122A61P11/06A61P11/02A61P17/00A61P27/02A61P37/08A61K8/35A61Q19/00A23L33/105
CPCA61K31/122A61P11/06A61P11/02A61P17/00A61P27/02A61P37/08A61K8/355A61Q19/00A23L33/105A23V2002/00A23V2200/324
Inventor 齐云邹忠梅高源蔡润兰张小雨李西蒙齐睿娟韩宜芯康媛
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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