Application of combined marker in preparation of product for evaluating effect of Guizhi Fuling capsule on treating primary dysmenorrhea

A technique for primary dysmenorrhea and cinnamon twigs and poria cocos, applied in the field of medicine, can solve complex problems that cannot explain the mechanism of traditional Chinese medicine treatment of diseases and syndromes well

Pending Publication Date: 2021-07-16
JIANGSU KANION PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complexity of both the traditional Chinese medicine system and the biological system of the body, these studies cannot well explain the mechanism of action of traditional Chinese medicine in treating diseases and syndromes. [5]

Method used

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  • Application of combined marker in preparation of product for evaluating effect of Guizhi Fuling capsule on treating primary dysmenorrhea
  • Application of combined marker in preparation of product for evaluating effect of Guizhi Fuling capsule on treating primary dysmenorrhea
  • Application of combined marker in preparation of product for evaluating effect of Guizhi Fuling capsule on treating primary dysmenorrhea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Network Regulatory Mechanism of Guizhi Fuling Capsules in Treating Primary Dysmenorrhea

[0041] The present inventor collected the ingredients contained in Guizhi Fuling Capsules from the literature, and used a high-precision drug target prediction method to predict the target spectrum of the ingredients in Guizhi Fuling Capsules. Enrichment analysis was used to calculate the biological functions affected by the compounds in Guizhi Fuling Capsules.

[0042] 1. Collection of compounds contained in Guizhi Fuling Capsules

[0043] Guizhi Fuling Capsules contain 5 traditional Chinese medicines: Guizhi, Poria, Cortex Moutan, Radix Paeoniae Alba, and Peach Kernel. [13] Compounds contained in various traditional Chinese medicines were collected in . A total of 122 compounds were collected, including 30 compounds contained in Guizhi, 33 compounds contained in Poria cocos, 66 compounds contained in Moutan bark, 57 compounds contained in Radix Paeoniae Alba, and 28 ...

Embodiment 2

[0048] Example 2: Guizhi Fuling Capsules Reduce Calcium Signals in Uterine Smooth Muscle Cells

[0049] 1. Experimental method

[0050]The experimental design was as follows: ICR female mice were injected with estradiol benzoate injection for two days (0.1 mg per mouse). ICR female mice were killed by cervical dislocation, sterilized with 75% alcohol, and the uteri were taken out and placed in PBS newly added with double antibodies. The fat and connective tissue were peeled off, the uterus was cut longitudinally, the endometrium was gently scraped off with a scalpel, and the endometrium was washed 1-2 times with PBS. Discard the buffer, cut the muscle strips into 1-2mm3 pieces with ophthalmic scissors, add 2ml 0.025g·L-1 collagenase II, transfer to the Erlenmeyer flask, and wash the scissors with 5 ml collagenase II. Transfer the Erlenmeyer flask to a 37°C water bath for digestion for 1 hour, and pipette for 5 minutes every half hour. An equal volume of complete medium cont...

Embodiment 3

[0053] Embodiment 3: Guizhi Fuling Capsule inhibits the substance of COX2 enzyme activity

[0054] 1. Experimental method

[0055] The experimental design is as follows: using the biological evaluation method based on fluorescence detection of human recombinant COX2 enzyme activity, it is detected that Guizhi Fuling Capsules can inhibit COX2 activity. According to the instructions of the cyclooxygenase 2 (COX2) inhibitor screening kit, the technology detects the effect of Guizhi Fuling capsule on COX2.

[0056] 2. Experimental results

[0057] The experimental results verified the inhibitory effect of Guizhi Fuling Capsules on the key enzyme COX2 in inflammation and pain, as shown in Figure 4. The above results suggest that Guizhi Fuling Capsules may exert anti-inflammatory and analgesic effects and improve primary dysmenorrhea by affecting the expression and activity of the key enzyme COX2 in inflammation and pain, thereby affecting the synthesis of prostacyclins (PGs) in t...

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Abstract

The Guizhi Fuling capsule for treating primary dysmenorrhea lack objective curative effect evaluation indexes clinically. Through calculation prediction and experimental verification of network pharmacology, it is found that the Guizhi Fuling capsule capsule regulates intracellular calcium ion influx and intervenes COX2 to play an anti-inflammatory and analgesic role to relieve primary dysmenorrhea, and it is found that the Guizhi Fuling capsule intervenes endocrine hormone and microcirculation, regulates intrauterine circulation and plays a lasting effect. The invention relates to application of a combined biological marker in preparation of a product for evaluating the effect of the Guizhi Fuling capsule on treating primary dysmenorrhea, wherein the combined biological marker consists of TXB2, 6-Keto-PGF1 alpha, COX2, NO, ET-1 and intracellular calcium ions. In-vitro experiments show that the Guizhi Fuling capsule can significantly reduce the ratio of TXB2 and TXB2/6-Keto-PGF1 alpha in plasma, significantly increase NO in uterine tissues, significantly reduce the level of ET-1, and inhibit COX2 enzyme activity and calcium ion influx in uterine smooth muscle cells. Clinical tests show that the pain relieving effect of the Guizhi Fuling capsule capsule is obviously superior to that of placebo, and the capsule has an obvious lasting effect after drug withdrawal.

Description

technical field [0001] The invention relates to the field of medicine, and relates to the application of a combination marker in the preparation of a product for evaluating the effect of Guizhi Fuling Capsules in treating primary dysmenorrhea. Background technique [0002] Primary dysmenorrhea (Primary Dysmenorrhea, PD) refers to a kind of functional dysmenorrhea caused by non-genital organic diseases, which seriously affects the normal work and quality of life of patients. [1] . Its pathogenesis involves changes in prostaglandins (PGs), oxytocin (OT), estrogen and progesterone, and β-endorphin (β-EP) [2] . [0003] Guizhi Fuling Capsules are composed of five flavors of traditional Chinese medicines: Guizhi, Poria, Cortex Moutan, Paeoniae Alba, and Peach Kernel. [3] . The pharmacological effects of Guizhi Fuling Capsules are diverse. It has been reported that Guizhi Fuling Capsules have a wide range of functions such as immune regulation, anti-tumor, anti-inflammation, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/88G01N33/84G01N33/74G01N33/573G01N33/53
CPCG01N33/94G01N33/88G01N33/84G01N33/74G01N33/573G01N33/5308G01N2333/90245G01N2800/361G01N2800/52
Inventor 李梢肖伟赖新星王振中张斯琴曹亮
Owner JIANGSU KANION PHARMA CO LTD
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