Method for preparing high-purity bakuchiol through high-speed countercurrent chromatography separation

Abstract

The invention relates to a method for preparing high-purity bakuchiol by high-speed countercurrent chromatography separation. The method comprises the following steps: soaking a fructus psoraleae medicinal material in an ethanol solution, and extracting to obtain a fructus psoraleae crude extract; fully shaking the solvent system, standing for phase splitting, and separately collecting an upper phase and a lower phase; dissolving the fructus psoraleae crude extract in the upper phase, separating by adopting high-speed counter-current chromatography to obtain a mixed solution of bakuchiol and a lower phase by taking the upper phase as a stationary phase and the lower phase as a mobile phase, and removing the lower phase to obtain bakuchiol. According to the method, the high-purity bakuchiol is separated and purified from the fructus psoraleae medicinal material by utilizing a high-speed counter-current chromatography technology for the first time, all reagents of a solvent system can be recycled, and a good environment-friendly function is achieved.

Description

technical field

[0001] The invention belongs to the technical field of cosmetic raw material processing, and in particular relates to a method for preparing high-purity bakuchiol through high-speed countercurrent chromatographic separation. Background technique

[0002] Psoralen is the dry ripe fruit of Psoralea corylifolia L., a leguminous plant. Bakuchiol is an important monoterpene compound in psoralen, which has various pharmacological activities such as whitening, antibacterial, anti-inflammatory, anti-aging, and anti-oxidation. How to prepare high-purity bakuchiol in a large amount simply and quickly is a problem closely watched in the industrial production as a cosmetic raw material.

[0003] Patent CN108299453A discloses a method for separating psoralen, isopsoralen and bakuchiol from psoralen, comprising the following steps: a) extracting the psoralen medicinal material with petroleum ether after crushing to obtain The petroleum ether crude extract of psoralen; b)...