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MoS2-IL@CS electrochemical chiral sensor for recognizing tryptophan enantiomers and application thereof

An enantiomer, mos2-il technology, applied in the field of electrochemical chiral sensing, can solve the problem of limited conductivity of MoS2, and achieve the effects of wide practical application value, simple construction method, and single detection step.

Pending Publication Date: 2021-08-03
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to MoS 2 has limited conductivity, so there are few studies on its use as electrode modification materials

Method used

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  • MoS2-IL@CS electrochemical chiral sensor for recognizing tryptophan enantiomers and application thereof
  • MoS2-IL@CS electrochemical chiral sensor for recognizing tryptophan enantiomers and application thereof
  • MoS2-IL@CS electrochemical chiral sensor for recognizing tryptophan enantiomers and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: MoS 2 -Synthesis and characterization of IL composites

[0024] 1. MoS 2 - Synthesis of IL composites

[0025] Dissolve 500 mg of sodium molybdate dihydrate in 50 mL of ultrapure water, then adjust the pH to 6.5 with hydrochloric acid. Subsequently, 1.00 g of L-cysteine ​​and 100 mL of ultrapure water were added, and sonicated for 10 min. The mixed solution was then transferred to a Teflon-lined 80 mL autoclave and heated at 200 °C for 36 h. After cooling to room temperature, the solid product was collected by centrifugation, washed with ultrapure water and ethanol, and then dried in vacuum at 80 °C for 8 h to prepare MoS 2 .

[0026] 0.04g 1-benzyl-3-methylimidazolium tetrafluoroborate ([BZMLM]BF 4 ) joined MoS 2 The aqueous dispersion was continuously stirred at 60°C for 12h, then stirred at 90°C for 1h and filtered with a filter membrane with a diameter of 0.2μm. After washing with ultrapure water, the MoS 2 -IL composites were vacuum dried at 60 ...

Embodiment 2

[0039] Example 2: MoS 2 - Preparation of IL@CS Electrochemical Chiral Sensor

[0040] 1. MoS 2 -IL@CS Composite Membrane Modified Working Electrode

[0041] A three-electrode system was adopted, with a glassy carbon electrode (GCE) as the working electrode, a platinum wire (Pt) as the counter electrode, and a saturated calomel electrode (SCE) as the reference electrode. After thoroughly cleaning the electrode and testing its performance to be qualified, add 15 μL of 2 mg / mL MoS dropwise on its surface 2 -After the IL dispersion, it is placed in room temperature and dried naturally, and it is recorded as MoS 2 -IL / GCE. Subsequently, CS (2g / L, 0.1mol / L HAc) with a viscosity of 100-200mpa.s was deposited at a constant potential (-0.5V) at 20°C to 30°C, and the electrodeposition time was 200s. Wash the remaining CS solution on the surface with water and let it dry naturally at room temperature to prepare MoS 2 -IL@CS / GCE.

[0042] 2. Electrochemical chiral recognition of tr...

Embodiment 3

[0044] Example 3: Detection of a racemic mixture of tryptophan enantiomers

[0045] MoS 2 -IL@CS / GCE were respectively immersed in 500 μmol / L of PBS (0.1mol / L, pH = 6.0) After incubating in the solution for 1 min, use differential pulse voltammetry (DPV) detection, 0.4 to 1.0 V, with a potential increment of 4 mV, and record the DPV peak position. Such as Figure 7 As shown, with the increase of D-Trp%, the peak potential shifts positively, and D-Trp% has a linear relationship with the DPV peak potential: y=835.6880+51.3844x, R 2 is 0.9959.

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Abstract

The invention relates to a MoS2-IL@CS electrochemical chiral sensor for recognizing tryptophan enantiomers and application thereof, which belong to the field of electrochemical chiral sensing. A MoS2-IL@CS composite film is modified on the surface of a working electrode to serve as a chiral selective agent, and tryptophan enantiomers are recognized by utilizing DPV peak potential difference. When the dropwise adding amount of 2 mg / mL MoS2-IL dispersion liquid is 15 muL, the electrodeposition time of CS with the viscosity of 100-200 mpa.S is 200 s, and the detection conditions of the tryptophan enantiomer are as follows: the temperature is 15 DEG C, and the pH value is 6.0, the DPV peak potential difference is maximum. The MoS2-IL@CS electrochemical chiral sensor is simple in construction method, low in professional requirement on operators and easy to popularize, can be used for quantifying the proportion of tryptophan enantiomers in a tryptophan racemic mixture, and has wide practical application value.

Description

technical field [0001] The invention belongs to the field of electrochemical chiral sensing, in particular to MoS 2 - Construction of IL@CS electrochemical chiral sensor and its application in recognition and detection of tryptophan (Trp) enantiomers. Background technique [0002] Chirality is an inherent property of most natural substances such as amino acids, proteins, and polysaccharides, and plays an important role in fields such as biomedicine, clinical diagnosis, chemical industry, and food safety. Enantiomers show huge differences in biological metabolism, physiological toxicity and pharmacological activity. Therefore, chiral recognition has always been a research hotspot. Electrochemical chiral recognition has been considered as an effective method to distinguish enantiomers due to its rapid response, high sensitivity, low cost and real-time analysis. In recent years, chitosan (CS), β-cyclodextrin, chiral metal-organic frameworks, chiral single-armed carbon nanotu...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/32G01N27/38G01N27/48
CPCG01N27/308G01N27/301G01N27/32G01N27/38G01N27/48
Inventor 罗爱芹侯慧鹏刘毅权汤波梁阿新唐珊珊王微
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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