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Tobacco black shank-resisting active compound in Thalictrum finetii Boivin as well as preparation method and application of tobacco black shank-resisting active compound

A technology of tobacco black shank and compound, which is applied in the field of phytochemistry, can solve the problems of undiscovered active substances, etc., and achieve the effects of good anti-black shank activity, low cost, and wide sources

Active Publication Date: 2021-08-06
YUNNAN MINZU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on Tangsong grass, and there may be undiscovered active substances in Tangsong grass.

Method used

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  • Tobacco black shank-resisting active compound in Thalictrum finetii Boivin as well as preparation method and application of tobacco black shank-resisting active compound
  • Tobacco black shank-resisting active compound in Thalictrum finetii Boivin as well as preparation method and application of tobacco black shank-resisting active compound
  • Tobacco black shank-resisting active compound in Thalictrum finetii Boivin as well as preparation method and application of tobacco black shank-resisting active compound

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0026] The present invention also provides the preparation method of described anti-tobacco black shank active compound, specifically comprises the following steps:

[0027] (1) Extraction: dry and pulverize the whole plant of Pinegrass tangensis, take the first solvent for extraction, concentrate, take the second solvent for extraction, the aqueous phase is saturated with sodium carbonate, then extract with the second solvent, combine and concentrate to obtain the extract paste;

[0028] (2) dissolving the extract in a third solvent, separating it through a silica gel column, and eluting with a gradient of chloroform: acetone, and collecting the eluted components with a volume ratio of chloroform: acetone of 9:1;

[0029] (3) The eluted fraction obtained in step 2 was separated by HPLC chromatography to obtain the crude product of the target compound, and finally the crude product of the target compound was purified with a Sephadex column to obtain the pure product of the tar...

Embodiment 1

[0044]The sample of Sichuan-Yunnan Tangsong grass in this example comes from Heqing County, Dali, Yunnan. The whole plant of Pinegrass tangensis from Sichuan and Yunnan was sun-dried and crushed to about 40 mesh. Weigh 3.0 kg of the pulverized sample, place it in a 20 L glass reaction kettle, add 6 L of 95% ethanol aqueous solution, reflux for 50 min, and filter out the extract; add 6 L of 95% ethanol aqueous solution to the residue again, Reflux for extraction for 50 min, and filter the extract. The two extracts were combined and concentrated to a small volume, then diluted with 6 L of 3% tartaric acid solution, and extracted twice with 6 L of ethyl acetate. After the extraction, the aqueous phase was saturated with sodium carbonate, extracted twice with 6 L of ethyl acetate, and the extracted ethyl acetate phases were combined and concentrated under reduced pressure to obtain 48.5 g of alkaloid part extract. The extract was mixed with 60g (80-100 mesh) crude silica gel, dr...

Embodiment 2

[0051] The samples of Pinegrass tangensis in Sichuan and Yunnan came from Binchuan County, Dali, Yunnan. Take the whole plant of Sichuan-Yunnan Tangsong grass, dry it, and crush it to about 40 mesh. Weigh 3.6 kg of the pulverized sample, place it in a 20 L glass reaction kettle, add 8 L of 95% ethanol aqueous solution, reflux for extraction for 40 min, and filter out the extract; add 8 L of 95% ethanol aqueous solution to the residue again , refluxed for 40 min, and filtered off the extract. The two extracts were combined and concentrated to a small volume, then diluted with 8 L of 3% tartaric acid solution, and extracted twice with 8 L of ethyl acetate. After the extraction, the aqueous phase was saturated with sodium carbonate, extracted twice again with 8 L of ethyl acetate, and the extracted ethyl acetate phases were combined and concentrated under reduced pressure to obtain 53.6 g of alkaloid part extract. The extract was mixed with 80g (80-100 mesh) crude silica gel, d...

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Abstract

The invention discloses a tobacco black shank-resisting active compound as shown in a formula (I) as well as a preparation method and application thereof. The preparation method comprises the following steps: with Thalictrum finetii Boivin as a raw material, conducting extracting with high-concentration methanol, high-concentration acetone / water or high-concentration ethanol / water, merging extracts, conducting filtering, and performing concentrating under reduced pressure to obtain an extract; packing the extract into a column by using a silica gel dry column-packing method and carrying out silica gel column chromatography; carrying out gradient elution by using a chloroform-acetone solution; and further separating and purifying the 9: 1 part of eluate by using high-pressure liquid chromatography to obtain a required quinoline alkaloid compound. The invention further discloses application of the compound, and activity tests show that the compound has a good inhibiting effect on tobacco black shank. The compound disclosed by the invention is novel in structure, has relatively good black shank resisting activity, and can be used as a lead compound for resisting tobacco black shank in research and development of biological pesticides used for preventing and treating tobacco black shank.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and in particular relates to a new compound with anti-tobacco black shank activity extracted from Dianchuan Tangsong grass and its preparation method, as well as its application in anti-tobacco black shank. Background technique [0002] Tobacco black shank is one of the most destructive diseases in tobacco production, also known as tobacco blight. Tobacco farmers call it "black stem madness", "black root" and "aconite disease". There are varying degrees of occurrence in all major tobacco-producing areas in China, among which Anhui, Shandong, and Henan provinces are historically severely affected areas; Yunnan, Guizhou, Sichuan, Hunan, Guangdong, Guangxi, Fujian and other southern tobacco areas are also quite common. At present, the prevention and control of black shank is mainly achieved through methods such as crop rotation, genetic improvement of varieties, and biological pesticides. Am...

Claims

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Application Information

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IPC IPC(8): C07D215/227A01N43/42A01P3/00
CPCC07D215/227A01N43/42
Inventor 胡秋芬李银科杨光宇周敏汪伟光董淼黄海涛刘欣孔维松李晶王晋许永
Owner YUNNAN MINZU UNIV
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