Portable sensor based on MOFs probe recognition and preparation method and application thereof

A portable, sensor technology, applied in the direction of analysis by chemical reaction of materials, material analysis by observing the effect on chemical indicators, color/spectral property measurement, etc. It can improve the practical application, the preparation procedure is simple, and the catalytic activity is good.

Active Publication Date: 2021-08-10
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional detection techniques for pesticide residues mainly include gas chromatography, liquid chromatography, chromatography-mass spectrometry, etc. Although these methods are reliable in results, they are cumbersome to operate and require large equipment
With the development of biosensing technology, rapid detection methods for pesticide residues using electrochemical, fluorescent, ultraviolet and other technologies combined with sensors have begun to appear. Although these rapid detection methods are simple to operate and time-consuming, they still require specific equipment that is not easily available to the public. poor practical applicability
[0004] With the popularity of smart phones, digital image colorimetry is a new visualization method; although it can realize the detection of safety factors in complex matrices of agricultural products, due to the acid and alkali resistance of biomolecules such as aptamers, antibodies, proteases, etc. Poor, easy to deactivate, difficult to purify and other shortcomings, it is difficult to be widely used

Method used

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  • Portable sensor based on MOFs probe recognition and preparation method and application thereof
  • Portable sensor based on MOFs probe recognition and preparation method and application thereof
  • Portable sensor based on MOFs probe recognition and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] S1. Preparation of MOFs mimic enzymes:

[0037] Weigh 0.5 mm of Co(NO 3 ) 2 •6H 2 O was dispersed in 25 mL of methanol solution, and 2 mm of dimethylimidazole was weighed and dispersed in 25 mL of methanol solution. Under the condition of magnetic stirring, the above two solutions were mixed, incubated at room temperature for 24 h, and then centrifuged and washed with methanol for 3 times, and vacuum-dried at 80°C to obtain MOFs mimic enzymes.

[0038] S2. Preparation of MIP-MOFs probes

[0039] Add 10 μLAPTES and 2 mL10%NH to 500 mgMOFs 3 • · h 2 O, add 10 mg thiacloprid and stir for 5 min, then add 5 mL TEOS and stir for 12 h. It was then washed five times with ethanol to remove the thiacloprid template. The MIP-MOFs probe can be obtained after vacuum drying at 80 °C.

[0040] S3. Construction of the portable sensor:

[0041] Prepare thiacloprid standard solutions with concentrations of 0.1 μM, 0.2 μM, 0.5 μM, 1 μM, 1.2 μM, 1.4 μM, and 2 μM, respectively mix...

Embodiment 2

[0045] S1: Preparation of MOFs mimic enzymes

[0046] Weigh 1 mm of Co(NO 3 ) 2 •6H 2 O was dispersed in 25 mL of methanol solution, and 4 mm of dimethylimidazole was weighed and dispersed in 25 mL of methanol solution. Under the condition of magnetic stirring, the above two solutions were mixed, incubated at room temperature for 24 h, and then centrifuged and washed with methanol three times, and vacuum-dried at 60 °C to obtain MOFs mimic enzymes.

[0047] S2. Preparation of MIP-MOFs probes

[0048] MOFs serve as carriers for the synthesis of MIP-MOFs. Briefly, 15 μLAPTES and 4 mL of 10% NH were added to 600 mg MOFs 3 •H 2 O, add 15 mg thiacloprid and stir for 10 min, then add 5 mL TEOS and stir for 24 h. It was then washed five times with ethanol to remove the thiacloprid template. The MIP-MOFs probe can be obtained after vacuum drying at 60 °C.

[0049] S2. Construction of portable sensor:

[0050] Prepare concentrations of 0.1 μM, 0.2 μM, 0.5 μM, 1 μM, 1.2 μM, 1....

Embodiment 3

[0054] S1: Preparation of MOFs mimic enzymes

[0055] Weigh 1.5 mm of Co(NO 3 ) 2 •6H 2 O was dispersed in 25 mL of methanol solution, and 6 mm of dimethylimidazole was weighed and dispersed in 25 mL of methanol solution. Under the condition of magnetic stirring, the above two solutions were mixed, incubated at room temperature for 24 h, and then centrifuged and washed with methanol three times, and dried in vacuum at 70 °C to obtain MOFs mimic enzymes.

[0056] S2. Preparation of MIP-MOFs probes

[0057] MOFs serve as carriers for the synthesis of MIP-MOFs. Briefly, 20 μLAPTES and 6 mL of 10% NH were added to 700 mg MOFs 3 •H 2 O, add 20 mg thiacloprid and stir for 15 min, then add 5 mLTEOS and stir for 36 h. It was then washed five times with ethanol to remove the thiacloprid template. The MIP-MOFs probe can be obtained after vacuum drying at 70 °C.

[0058] S3. Construction of portable sensor:

[0059] Prepare concentrations of 0.1 μM, 0.2 μM, 0.5 μM, 1 μM, 1.2 μM...

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Abstract

The invention provides a portable sensor based on MOFs probe recognition and a preparation method and application thereof, which belong to the technical field of sensor preparation and rapid detection. Thiacloprid molecules and APTES TEOS are subjected to aggregation on the surface of MOFs to form an MIP-MOFs probe, then the visual portable sensor is constructed based on the probe, and the visual portable sensor can specifically identify and accurately and rapidly detect pesticide residues in matcha.

Description

technical field [0001] The invention belongs to the technical field of sensor preparation and rapid detection, and in particular relates to a portable sensor based on MOFs probe identification and its preparation method and application. Background technique [0002] Compared with the traditional method of "making tea and drinking soup", matcha has been favored by more and more consumers due to its unique flavor and the eating characteristics of "eating tea" directly, but the tea tree cultivation method is special and the growth environment is warm and humid , prone to various pests and diseases. At present, chemical pesticides are mainly used to prevent and control tea plant diseases and insect pests, which has caused matcha pesticides to exceed the standard and have many residues, which in turn leads to health risks and export returns, which seriously restricts the healthy and green development of my country's matcha industry and foreign trade. Therefore, it is particularl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N21/78
CPCG01N21/33G01N21/78
Inventor 张新爱皇雪月石吉勇邹小波黄晓玮李志华张迪申婷婷
Owner JIANGSU UNIV
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