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Method for screening methylation modification genes related to content of unsaturated fatty acid in oysters

An unsaturated fatty acid and modified gene technology is applied in the field of screening oyster unsaturated fatty acid content-related methylation modified genes, which can solve the problems of inability to achieve precise breeding of oyster unsaturated fatty acid content, low breeding accuracy and efficiency, and the like. Improve the quality and economic benefits of the industry, and solve the effects of low breeding accuracy and high polymorphism

Active Publication Date: 2021-08-13
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, studies on the unsaturated fatty acid content of oysters mostly use traditional genetic methods such as genomic selection, GWAS, and QTL to analyze the genetic mechanism of phenotype and genotype. The phenotype has a significant impact, but the traditional genetics-based oyster breeding ignores this part of the effect, resulting in low breeding accuracy and efficiency, and it is impossible to achieve precise breeding for the unsaturated fatty acid content of oysters
At present, there is no report on the influence mechanism of oyster DNA methylation and other epigenetic mechanisms on oyster unsaturated fatty acid content. Therefore, there is an urgent need for methods to screen methylation-modified genes related to quality traits such as oyster unsaturated fatty acid content.

Method used

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  • Method for screening methylation modification genes related to content of unsaturated fatty acid in oysters
  • Method for screening methylation modification genes related to content of unsaturated fatty acid in oysters
  • Method for screening methylation modification genes related to content of unsaturated fatty acid in oysters

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Experimental program
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Effect test

Embodiment 1

[0028] Take 100 long oysters with mature gonads, remove the right shell, identify the sex of each individual with a microscope, obtain 42 females and 48 males, dissect and mature the male and female gametes, and then mix the female gametes and homogenize them. Divided into the same number as the number of male parents, adding an appropriate amount of sperm from a male individual to each mixed gamete, and each fertilized egg was hatched separately to D-type larvae and then mixed in equal amounts to form the desired population.

[0029] The spat with a shell height of 0.5-5cm are equally divided into 5 parts, so that the genetic basis of each group of samples is consistent, and they are respectively placed in 4 tidal levels and subtidal zones of the intertidal zone of Xuejiadao wharf, specifically the intertidal zone 380cm ( In380), 300cm (In300), 200cm (In200), 100cm (In100) and the subtidal zone (Sub) 1m below the water surface. After the natural growth period of 2-3 months, t...

Embodiment 2

[0041] Take adult oysters with complete shells and heat them up indoors to promote ripening. When the gonads are mature, 120 oysters are randomly selected, and the right shells are removed respectively. The sex of each individual is identified with a microscope, and 48 females and 72 males are obtained. Individuals, male and female gametes were dissected and matured separately, and then the female gametes were mixed and divided into 72 parts, and an appropriate amount of sperm from a male individual was added to each mixed gamete, and each fertilized egg was hatched into D-type larvae separately. The amount of mixture was mixed, and the larvae were cultivated by the conventional oyster seed production process, and the required population was obtained after attachment and metamorphosis.

[0042] The spat with a shell height of 0.5-5cm were divided into 4 parts, and placed in the intertidal zone and subtidal zone of Xuejiadao Wharf, Jimo Sea Area and Rongcheng Sea Area respective...

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Abstract

The invention belongs to the field of epigenetic breeding of shellfish, and particularly relates to a method for screening methylation modification genes related to the content of unsaturated fatty acid in oysters. The method comprises the following steps of constructing an oyster colony for screening the methylation modification genes related to the content of the unsaturated fatty acid, randomly selecting part of juvenile mollusks, breeding the juvenile mollusks in different sea area environments for 20 days-120 days, detecting the content of the unsaturated fatty acid of the juvenile mollusks, selecting individuals with the largest unsaturated fatty acid content difference in two environments to respectively form 3-6 parallel group samples, analyzing candidate genes with significant differences in methylation and transcription levels between the two environments by adopting a multi-omic method, and carrying out candidate methylation regulation gene verification on the obtained candidate genes by adopting a real-time quantitative PCR and BSP method to obtain the methylation modification genes related to the content of the unsaturated fatty acid in the oysters. The method can efficiently screen the methylation modification genes related to the content of the unsaturated fatty acids in the oysters, and can provide important support for construction of an epigenetic breeding technology of the oysters.

Description

technical field [0001] The invention belongs to the field of epigenetic breeding of shellfish, and in particular relates to a method for screening methylation modification genes related to unsaturated fatty acid content in oysters. Background technique [0002] Oyster is one of the most important marine cultured shellfish in the world. my country's oyster production ranks first in the world for many years, accounting for 81% of the world's production in the same period. Oysters are rich in a variety of unsaturated fatty acids, which are important determinants of oyster quality and play an important role in human health. They can reduce blood cholesterol and triglycerides, improve blood microcirculation, increase brain cell activity, and enhance memory and thinking. ability etc. [0003] Oysters are naturally distributed in the shallow waters of intertidal and subtidal zones, and the complex and drastic changes in environmental conditions such as temperature, salinity, and d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6809C12Q1/6888
CPCC12Q1/6809C12Q1/6888C12Q2600/124C12Q2600/154C12Q2531/113C12Q2535/122C12Q2563/107C12Q2537/165Y02A40/81
Inventor 丛日浩李莉张国范赵泽民亓海刚黎奥王新星王威王鲁平
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI