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Rosa laevigata flower extract as well as medicine thereof, and preparation method and application thereof

A kind of white wood fragrance flower and extract technology, applied in the field of herbal medicine and medicine

Active Publication Date: 2021-08-17
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There has not been any report on the anti-tumor research of A. sinensis

Method used

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  • Rosa laevigata flower extract as well as medicine thereof, and preparation method and application thereof
  • Rosa laevigata flower extract as well as medicine thereof, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Preparation of PXS65.

[0020] Take white wood incense [ Aquilaria sinensis (Lour.) Spreng.] 50 g of dried flower samples were crushed and extracted with distilled water (0.5 L), ultrasonically extracted in a 60°C water bath for 30 min, repeated 3 times, combined and filtered, and the filtrate was discarded. For the sample extracted with distilled water, continue to add 90% ethanol, ultrasonically extract in a 60°C water bath for 30 min, repeat 3 times, combine and filter, recover the solvent, and obtain 2.43 g of ethanol extract (code PXS65).

Embodiment 2

[0022] Preparation of PXS66, PXS66-1, PXS66-2, PXS66-3 and PXS66-4.

[0023] Take white wood incense [ Aquilaria sinensis (Lour.) Spreng.] dried flower sample 1.15 kg, crushed and extracted with 90% ethanol (2 L), ultrasonically extracted in a 60°C water bath for 30 min, repeated 4 times, combined and filtered, recovered the solvent, and obtained the ethanol extract ( No. PXS66) 174.2 g. Take 172.0 g of PXS66, add water (1 L) to make a suspension, extract with petroleum ether (1 L × 3), recover petroleum ether, and obtain 21.15 g of petroleum ether extraction fraction (No. PXS66-1); after petroleum ether extraction Continue to extract the aqueous phase solution with ethyl acetate (1 L × 3), recover ethyl acetate, and obtain 23.0 g of the ethyl acetate extraction fraction (No. PXS66-2); the aqueous phase solution after ethyl acetate extraction, continue to use Extracted with n-butanol (1 L × 3), recovered n-butanol, and obtained 54.03 g of n-butanol extraction fraction (No. ...

Embodiment 3

[0025] Inhibitory effect of PXS65 on cancer cell growth.

[0026] Cancer cytotoxic activity was tested by MTS method [Yang J, Su Y, Luo J-F, Gu W, Niu H-M, LiY, Wang Y-H, Long C-L. New amide alkaloids from Piper longumfruits. Natural Products and Bioprospecting, 2013, 3, 277-281], cisplatin and paclitaxel were used as positive controls. The results of the cytotoxic activity test are shown in Table 1. The results showed that PXS65 was effective against lung cancer SPC-A-1, lung cancer NCI-H520, lung cancer A549, cervical cancer Hela, human neuroblastoma SH-SY5Y, ovarian cancer SK-OV-3, leukemia MT4 and prostate cancer PC-3, etc. Cancer cell lines have significant inhibitory activity, the half inhibitory concentration (IC 50 ) were less than 1 μg / mL; the inhibitory effect on cancer cell lines such as colon cancer SW480, breast cancer MCF-7, leukemia HL-60 and colon cancer Caco2, as well as human normal lung epithelial BEAS-2B cells was weak, and its IC 50 The value is greate...

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Abstract

The invention provides a botanical drug active part of aquilaria sinensis (Lour.) Spreng., a preparation method thereof, a pharmaceutical composition composed of the active part, and application of the botanical drug active part and the pharmaceutical composition in preparation of drugs for treating tumors or cancers. The preparation method comprises the following steps: extracting and extracting dried flos aquilaria sinensis by using a solvent to prepare PXS65, PXS66, PXS66-1, PXS66-2 and PXS66-3; the pharmaceutical composition has remarkable inhibitory activity on cancer cell strains, such as lung cancer SPC-A-1 cells, lung cancer NCI-H520 cells, lung cancer A549 cells, cervical cancer HeLa cells, human neuroblastoma SH-SY5Y cells, ovarian cancer SK-OV-3 cells, leukemia MT4 cells, prostate cancer PC-3 cells, liver cancer SMMC-7721 cells, breast cancer MDA-MB-231 cells, lung cancer NCI-H446 cells, lung cancer taxol drug-resistant strain A549 / Taxol cells and the like. The flos aquilaria sinensis extract disclosed by the invention provides a new choice for drugs for treating or preventing tumors or cancers and drugs for improving tumor or cancer drug resistance.

Description

Technical field: [0001] The present invention belongs to the technical field of medicine, in particular, relates to the technical field of herbal medicine, and more specifically, relates to the extract and pharmaceutical composition thereof, its preparation method and its application in the preparation of medicines for treating or preventing tumors or cancers. Background technique: [0002] Shiraki [ Aquilaria sinensis (Lour.) Spreng.] is a plant of the genus Agarwood in the family Daphneaceae, and its heartwood containing resin is the traditional Chinese medicine agarwood. At present, the research on the heartwood of A. argentina is relatively extensive, but the research on A. argentina is less, except that there is a document reporting the chemical components in the essential oil of A. argentina [Mei Wenli, Lin Feng, Dai Haofu. The composition of volatile oils in A. argentina and its fruit GC-MS analysis, Journal of Tropical and Subtropical Botany, 2009, 17(3), 305-308],...

Claims

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Application Information

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IPC IPC(8): A61K36/835A61P35/00A61P35/02A61K135/00
CPCA61K36/835A61P35/00A61P35/02A61K2236/15A61K2236/331A61K2236/39A61K2236/333A61K2236/53A61K2236/51Y02A50/30
Inventor 杨珺王跃虎罗吉凤夏梦媛张云峰
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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