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Gel preparation kit, injectable hydrogel and application of injectable hydrogel

A kit and water injection technology, applied in the gel preparation kit, injectable hydrogel field, to achieve the effects of improving prognosis, inhibiting inflammatory response, and improving cerebral hemorrhage

Pending Publication Date: 2021-08-17
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports of injectable hydrogels that meet the above requirements at the same time

Method used

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  • Gel preparation kit, injectable hydrogel and application of injectable hydrogel
  • Gel preparation kit, injectable hydrogel and application of injectable hydrogel
  • Gel preparation kit, injectable hydrogel and application of injectable hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, the preparation of injectable hydrogel of the present invention

[0041]Theoretically, the biomimetic injectable hydrogel is prepared by mixing 3% w / v G-SH:1% w / v HA-SH at a ratio of 90%:10%. That is, when the total volume is 100 μl, 90 μl Gel-SH (3% w / v) + 10 μl HA-SH (1% w / v) to form a ratio, and the PEGDA powder of the total mass fraction of the two was added respectively. In order to control the gelation time conveniently, the preparation method of dissolving each component separately and then mixing them was adopted: that is, 2.8mg PEGDA powder, 2.7mg Gel-SH and 0.1mg HA-SH freeze-dried powder were weighed and put into sterile 1.5 1 ml of EP tube, take 100 μl sterile PBS, add 20 μl of it to the EP tube containing PEGDA powder, and add 40 μl to the EP tube containing Gel-SH and HA-SH freeze-dried powder respectively. After the three are completely dissolved, transfer the Gel-SH solution to the HA-SH solution, mix well, then transfer the mixture to the...

Embodiment 2

[0042] Embodiment 2, preparation of injectable hydrogel of the present invention

[0043] The biomimetic injectable hydrogel is theoretically mixed according to the ratio of 3% w / v G-SH: 1% w / v HA-SH to 70%: 30%, that is, when the total volume is 100 μl, 70 μl Gel-SH ( 3% w / v) + 30 μl HA-SH (1% w / v) to form a ratio, and the PEGDA powder of the total mass fraction of the two was added respectively. In order to control the gelation time conveniently, the preparation method of dissolving each component separately and then mixing them was adopted: that is, 2.4mg PEGDA powder, 2.1mg Gel-SH and 0.3mg HA-SH freeze-dried powder were weighed and put into sterile 1.5 In the ml EP tube, take 100 μl of sterile PBS, add 20 μl of it to the EP tube containing PEGDA powder, and add 40 μl to the EP tubes containing Gel-SH and HA-SH freeze-dried powder respectively. After the three are completely dissolved, transfer the Gel-SH solution to the HA-SH solution, mix well, then transfer the mixture...

Embodiment 3

[0044] Embodiment 3, the preparation of injectable hydrogel of the present invention

[0045] Theoretically, the biomimetic injectable hydrogel is prepared by mixing 3% w / v G-SH:1% w / v HA-SH at a ratio of 50%:50%. That is, when the total volume is 100 μl, 50 μl Gel-SH (3% w / v) + 50 μl HA-SH (1% w / v) to form a ratio, and the PEGDA powder of the total mass fraction of the two was added respectively. In order to control the gelation time conveniently, the preparation method of dissolving each component separately and then mixing them was adopted: that is, 2.0 mg PEGDA powder, 1.5 mg Gel-SH and 0.5 mg HA-SH freeze-dried powder were weighed and put into sterile 1.5 In the ml EP tube, take 100 μl of sterile PBS, add 20 μl of it to the EP tube containing PEGDA powder, and add 40 μl to the EP tubes containing Gel-SH and HA-SH freeze-dried powder respectively. After the three are completely dissolved, transfer the Gel-SH solution to the HA-SH solution, mix well, then transfer the mixt...

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Abstract

The invention provides a gel preparation kit and injectable hydrogel prepared by using the kit. The kit comprises 2.1-2.7 parts by weight of sulfhydrylated gelatin, 0.1-0.3 part by weight of sulfhydrylated hyaluronic acid, 2.4-2.8 parts by weight of a cross-linking agent and 100 parts by volume of a solvent, and the solvent is water, a PBS buffer solution or an artificial cerebrospinal fluid. The modulus of the injectable hydrogel is matched with that of brain tissues, the degradation rate is proper, the formation of glial scars after cerebral hemorrhage can be controlled while inflammatory response is inhibited, and the adverse effect of the glial scars on axon growth and remyelination is relieved. A nerve repair promoting factor can be further added, so that endogenous nerve repair and neurological function recovery are effectively promoted, the cerebral hemorrhage prognosis is improved, and the application value is excellent.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a gel preparation kit, an injectable hydrogel and applications thereof. Background technique [0002] Intracerebral hemorrhage (ICH) is characterized by non-traumatic hemorrhage of the brain parenchyma. Compared with ischemic strokes of the same size, blood accumulation in the parenchyma will lead to more severe neuronal cell death and inflammation in the acute phase. Occurs, and various permanent impairments may be left behind in the later stage, including cognitive deficits or sensorimotor disturbances. Most of the researches on cerebral hemorrhage in the past focused on the prevention and protection of hematoma removal in the acute phase and the corresponding nerve damage, but little progress has been made on the promotion of functional recovery. [0003] Under normal physiological conditions, there will be a certain amount of neural stem cells (Neural Stem Cel...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/18A61L27/22A61L27/50A61L27/52A61L27/54A61L27/58
CPCA61L27/20A61L27/222A61L27/18A61L27/227A61L27/50A61L27/52A61L27/54A61L27/58A61L2430/32A61L2400/06A61L2300/414A61L2300/254A61L2300/412C08L5/08C08L89/00C08L71/02
Inventor 田猛许家科马潞李浩游潮
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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