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Xanthobacter sp. T21 and application thereof

A bacillus and yellow technology, applied in bacteria, microorganisms, biochemical equipment and methods, etc., can solve problems such as refractory degradation, and achieve the effect of strong environmental adaptability, huge potential for ecological restoration, and efficient degradation.

Active Publication Date: 2021-08-17
GUANGZHOU INST OF GEOCHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Organic phosphates can be hydrolyzed by microorganisms, but TCEP is difficult to be degraded by microorganisms due to its physical and chemical properties

Method used

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  • Xanthobacter sp. T21 and application thereof
  • Xanthobacter sp. T21 and application thereof
  • Xanthobacter sp. T21 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Isolation and purification of strain T21

[0021] The sediment samples (ie, sediments) used for the isolation of microorganisms were obtained near an organophosphate flame retardant manufacturer in Hebei.

[0022] First, the sediment was enriched and cultured with mineral salt medium (MSM), and the specific steps were as follows: 10 g of sediment was added to a 250 mL Erlenmeyer flask filled with 90 mL of inorganic salt medium, and 5 mg / L TCEP was added as the only carbon source. Place a shaker, culture at 25°C, 120rpm. After TCEP is completely degraded, add TCEP to a concentration of 50 mg / L to continue culturing. After TCEP is completely degraded, transfer it to a new inorganic salt medium at a ratio of 1% by volume. After the transfer, add 50 mg / L TCEP as the sole carbon source, place on a shaker, and culture at 25°C and 120rpm. After TCEP is completely degraded, add 50mg / L TCEP to continue the cultivation, repeat the cultivation, and after TCEP is completely de...

Embodiment 2

[0028] Example 2 Efficiency Measurement of Flavobacterium T21 Degradation TCEP

[0029] Inoculate Xanthobacterium T21 into LB medium, culture at 25°C and 120rpm until OD600 is about 0.1, obtain bacterial liquid, collect bacterial cells by centrifugation, wash 3 times with inorganic salt medium (same as Example 1), and resuspend in two Inorganic salt culture medium (same as Example 1) with double the bacterial liquid volume, add 50mg / L TCEP, 25 ℃, 120rpm culture. A control group without addition of Xanthobacterium T21 was also set up. Each experimental group contained three replicate samples. The results showed that the degradation rate of 50mg / L TCEP was 100% within 120 minutes, and the half rate constant was 22.4 minutes. TCEP degradation did not occur in the control group without strain T21. The degradation kinetics curve is as figure 2 shown.

Embodiment 3

[0030] The influence of embodiment 3 temperature and pH on the degradation rate of Xanthobacterium T21

[0031] Inoculate Xanthobacterium T21 into LB medium, culture at 25°C, 120rpm until OD600 is about 0.1, obtain bacterial liquid, collect bacterial cells by centrifugation, wash 3 times with inorganic salt medium (same as Example 1), and resuspend in bacterial The same volume of inorganic salt culture medium (same as Example 1), add 50mg / L TCEP, set different gradient culture temperatures (20°C, 25°C, 30°C, 35°C, 40°C), and take samples after 30 minutes of shaking at 120rpm Determine the remaining TCEP concentration. Each culture condition contained three replicate samples.

[0032]Inoculate Xanthobacterium T21 into LB medium, culture at 25°C, 120rpm until OD600 is about 0.1, obtain bacterial liquid, collect bacterial cells by centrifugation, wash with inorganic salt medium (same as Example 1) for 3 times, and resuspend in bacterial The same volume of inorganic salt culture...

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Abstract

The invention discloses a strain of xanthobacter sp. T21 and application thereof. The strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on January 22, 2021, and has an accession number of GDMCC No: 61462. The Xanthobactersp. T21 disclosed by the invention is obtained through enrichment with TCEP as a unique carbon source, can efficiently degrade the TCEP, has relatively strong environmental adaptability, and presents degradation action on the TCEP under the conditions that a temperature range is 20-40 DEG C and a pH range is 4-9. Under a neutral and slightly alkaline condition, the degradation efficiency of the Xanthobacter sp. T21 on TCEP with a concentration of 50 mg / L can reach 80%-98% within 30 minutes, and the Xanthobacter sp. T21 has efficient degradation capacity on TCEP and has huge potential when applied to ecological restoration of organic phosphate pollution.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a Xanthobacter sp. T21 strain capable of efficiently degrading tris(2-chloroethyl) phosphate (TCEP) and its application. Background technique [0002] As a substitute for brominated flame retardants, organic phosphates have been widely used in building materials, electronic equipment, and home decorations in recent years. The output has increased year by year, and the scope of use has greatly increased. At present, organophosphate pollution is commonly detected in various environmental media, including surface water, sediment, and indoor environment. Organophosphates and metabolites were also detected in organisms (fish, birds) and human samples (urine, breast milk, blood). In the past decade, the impact of organophosphates on public health and the ecological environment has received widespread attention worldwide. Toxicological studies have found that tris(2-chloroethy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02A62D101/28C12R1/01
CPCC12N1/20A62D3/02A62D2101/28
Inventor 梁医周翔昱张彪吴毅钉曾祥英于志强
Owner GUANGZHOU INST OF GEOCHEMISTRY - CHINESE ACAD OF SCI
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