A strain of Xanthobacterium t21 and its application
A Xanthobacterium, T21 technology, applied in the direction of bacteria, microorganisms, microorganism-based methods, etc., can solve problems such as refractory degradation, and achieve the effects of strong environmental adaptability, efficient degradation, and high-efficiency degradation ability
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Embodiment 1
[0020] 1. Isolation and purification of strain T21
[0021] The sediment samples (ie, sediments) used for the isolation of microorganisms were obtained near an organophosphate flame retardant manufacturer in Hebei.
[0022] First, the sediment was enriched and cultured with mineral salt medium (MSM), and the specific steps were as follows: 10 g of sediment was added to a 250 mL Erlenmeyer flask filled with 90 mL of inorganic salt medium, and 5 mg / L TCEP was added as the only carbon source. Place a shaker, culture at 25°C, 120rpm. After TCEP is completely degraded, add TCEP to a concentration of 50 mg / L to continue culturing. After TCEP is completely degraded, transfer it to a new inorganic salt medium at a ratio of 1% by volume. After the transfer, add 50 mg / L TCEP as the sole carbon source, place on a shaker, and culture at 25°C and 120rpm. After TCEP is completely degraded, add 50mg / L TCEP to continue the cultivation, repeat the cultivation, and after TCEP is completely de...
Embodiment 2
[0028] Example 2 Efficiency Measurement of Flavobacterium T21 Degradation TCEP
[0029] Inoculate Xanthobacterium T21 into LB medium, culture at 25°C and 120rpm until OD600 is about 0.1, obtain bacterial liquid, collect bacterial cells by centrifugation, wash 3 times with inorganic salt medium (same as Example 1), and resuspend in two Inorganic salt culture medium (same as Example 1) with double the bacterial liquid volume, add 50mg / L TCEP, 25 ℃, 120rpm culture. A control group without addition of Xanthobacterium T21 was also set up. Each experimental group contained three replicate samples. The results showed that the degradation rate of 50mg / L TCEP was 100% within 120 minutes, and the half rate constant was 22.4 minutes. TCEP degradation did not occur in the control group without strain T21. The degradation kinetics curve is as figure 2 shown.
Embodiment 3
[0030] The influence of embodiment 3 temperature and pH on the degradation rate of Xanthobacterium T21
[0031] Inoculate Xanthobacterium T21 into LB medium, culture at 25°C, 120rpm until OD600 is about 0.1, obtain bacterial liquid, collect bacterial cells by centrifugation, wash 3 times with inorganic salt medium (same as Example 1), and resuspend in bacterial The same volume of inorganic salt culture medium (same as Example 1), add 50mg / L TCEP, set different gradient culture temperatures (20°C, 25°C, 30°C, 35°C, 40°C), and take samples after 30 minutes of shaking at 120rpm Determine the remaining TCEP concentration. Each culture condition contained three replicate samples.
[0032]Inoculate Xanthobacterium T21 into LB medium, culture at 25°C, 120rpm until OD600 is about 0.1, obtain bacterial liquid, collect bacterial cells by centrifugation, wash with inorganic salt medium (same as Example 1) for 3 times, and resuspend in bacterial The same volume of inorganic salt culture...
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