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A kind of halovibrio using volatile fatty acid and its application

A technology of halovibrio and polyhydroxyalkanoate, applied in the field of microbiology and fermentation engineering, can solve the problems of limiting the large-scale industrial production and application of PHA, and high production costs

Active Publication Date: 2022-04-08
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although PHA has good application prospects, its large-scale industrial production and application of PHA are limited due to its production cost being significantly higher than that of petroleum-derived plastics.

Method used

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  • A kind of halovibrio using volatile fatty acid and its application
  • A kind of halovibrio using volatile fatty acid and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1, the separation and identification of Salinivibrio kushneri TGB-4

[0063] 1. Separation of Salinivibrio kushneri TGB-4

[0064] Collect water samples from salt ponds in the salt field of Tianjin Binhai New Area. Take 1 mL of water sample and add it to TYS liquid medium containing 20g / L acetic acid, incubate at 30°C and 200rpm for 24h, use a sterilized inoculation loop to streak the TYS solid medium containing 20g / L acetic acid, and incubate for 24h upside down. Pick a single clone and purify it on a TYS solid medium plate. After confirming that it is a pure bacteria, transfer it to a slant for short-term storage at 4°C, and transfer it to a 25% glycerol tube for long-term storage at -80°C. One of the isolated and purified strains TGB-4 is Salinivibrio kushneri TGB-4.

[0065] 2. Identification of Salinivibrio kushneri TGB-4

[0066] (1) Observation of culture traits of strain TGB-4

[0067] After the strain TGB-4 was inoculated on TYS medium and cultured ...

Embodiment 2

[0076] Embodiment 2: Utilize acetic acid to produce polyhydroxyalkanoate

[0077]1. Preparation of Salinivibrio kushneri TGB-4 seed liquid by aseptic operation

[0078] (1) Activation of bacteria

[0079] Take Salinivibrio kushneri TGB-4 glycerol tubes stored in a -80°C refrigerator, streak and inoculate them on TYS solid medium plates, and incubate at 30°C for 16 hours.

[0080] (2) Preparation of seed solution

[0081] Pick a single colony from the plate that has completed step (1), inoculate it in TYS liquid medium, and culture it with shaking at 30° C. and 200 rpm for 12 hours to obtain a seed solution.

[0082] 2. There are two treatments in the experiment: the experimental group and the control group.

[0083] Experimental group: inoculate the seed solution obtained in step 1(2) into 20g / L acetic acid+TYS liquid medium according to the inoculation amount of 2.5% (that is, 1mL of seed solution and 39mL of medium), use a 250mL shake flask, and assemble The liquid volum...

Embodiment 3

[0091] Embodiment 3: Utilize butyric acid to produce polyhydroxyalkanoate

[0092] The specific operation is according to Example 2 of the present invention, the difference is that the medium in the experimental group is a liquid TYS medium containing 15 g / L butyric acid. The culture medium of the control group was liquid TYS medium without butyric acid.

[0093] By calculation, when butyric acid is the carbon source, the dry cell weight is 2.29g / L, and the polyhydroxyalkanoate accumulated in the cell is poly-3-hydroxybutyrate, and the output is 0.92g / L, accounting for 1% of the dry cell weight. The mass fraction is 40.26%.

[0094] In addition, utilize transmission electron microscope to observe after the bacteria in embodiment 2 are cultivated, and the results are as follows: figure 1 with figure 2 Shown, where the scale bar is 200nm. figure 1 When butyric acid was used as a carbon source, the bacteria accumulated a large number of white polyhydroxyalkanoate particles i...

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Abstract

The invention discloses a halovibrio using volatile fatty acid and application thereof. The Salinivibriokushneri of the present invention is Salinivibriokushneri, its strain number is TGB-4, and its preservation number in the General Microorganism Center of China Microbiological Culture Collection Management Committee is CGMCC No.21105. The application of the halovibrio described in the present invention in any of the following: 1) production of polyhydroxyalkanoate; 2) preparation of products for the production of polyhydroxyalkanoate. The halovibrio of the present invention can use volatile fatty acid raw materials (acetic acid, butyric acid, etc.) as a carbon source to grow rapidly and accumulate poly-3-hydroxybutyrate. The strain was able to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) upon addition of propionic acid. The method of the present invention uses volatile fatty acid as a carbon source to produce polyhydroxyalkanoate, can reduce the fermentation cost of polyhydroxyalkanoate, and has great economic advantages and good industrial application prospects.

Description

technical field [0001] The invention belongs to the field of microbiology and fermentation engineering, relates to a halovibrio utilizing volatile fatty acid and its application, in particular to a halovibrio utilizing volatile fatty acid and its application in the production of polyhydroxyalkanoate. Background technique [0002] Plastics and their products have spread across all fields of national economy and social life, and have played a huge and irreplaceable role in people's daily life. The world's plastic production in 2018 was close to 360 million tons, and China's plastic production in 2019 exceeded 80 million tons, most of which were non-degradable plastic materials. It is estimated that about 5 billion tons of plastic products have been landfilled or abandoned in the environment, posing a great threat to the health of wild animals and humans themselves. The development and utilization of biodegradable plastic materials to reduce garbage pollution is the requiremen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/625C12R1/01
CPCC12N1/20C12P7/625
Inventor 李正军陶观宝
Owner BEIJING UNIV OF CHEM TECH
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