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A kind of frangipani extract and its preparation method and application

A technology of frangipani flower and water extract, which is applied in the new application field of frangipani flower water extract, can solve the problems such as no report on the activity of frangipani flower against Stenotrophomonas maltophilia, etc., and achieves the reduction of dosage and the improvement of comprehensive utilization. Effect

Active Publication Date: 2022-03-29
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many studies on the anti-inflammatory and antiviral effects of frangipani, but there is no report on the activity of frangipani against Stenotrophomonas maltophilia

Method used

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  • A kind of frangipani extract and its preparation method and application
  • A kind of frangipani extract and its preparation method and application
  • A kind of frangipani extract and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1: preparation of frangipani water extract

[0044] Take 200g of frangipani flowers, grind them into coarse powder, add 1000mL of distilled water, shake well, soak for 1h, reflux for 2-4h, pour out the extract, then add 1000mL of distilled water, reflux for 2h, combine the extracts, suction filter, 60℃ Concentrate under reduced pressure to 250 mL, and freeze-dry to obtain frangipani water extract.

Embodiment 2

[0045] Embodiment 2: in vitro antibacterial experiment

[0046] 1. Experimental method

[0047] Strains: Five strains of Stenotrophomonas maltophilia (SMA, numbered SMA1-SMA5) were clinically isolated.

[0048] The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Frangipani frangipani water extract against Stenotrophomonas maltophilia were determined by broth double dilution method.

[0049] 1. Determination of minimum inhibitory concentration (MIC)

[0050] (1) Preparation of bacterial suspension

[0051] Dip fresh cultures of SMA strains with sterile cotton swabs respectively, and make bacterial suspension with 0.9% physiological saline solution, the concentration is 1×10 8 cfu / mL.

[0052] (2) Double dilution method

[0053]Using 5mL EP tubes, add 2mL of broth medium to each tube. Frangipani water extract, levofloxacin, tetracycline, and ceftazidime were diluted serially in LB broth, and the inoculum was adjusted to 5×10 5 CFU / mL...

Embodiment 3

[0070] Example 3: Investigation of the sensitization effect of frangipani water extract on levofloxacin, tetracycline and ceftazidime against Stenotrophomonas maltophilia

[0071] 1. Checkerboard dilution method

[0072] Using the checkerboard dilution method, in a 48-well plate, a series of doubling dilutions were performed on the frangipani water extract, levofloxacin, tetracycline, and ceftazidime in LB broth medium, and the inoculum was adjusted to 5×10 after the corresponding drugs were compatible. 5 CFU / mL. Incubate each well plate at 37°C for 24 hours, and observe the MIC of the drug after compatibility. The lowest antibacterial drug concentration at which no precipitation occurs is the MIC.

[0073] The interaction between frangipani water extract and antibacterial drugs was evaluated by calculating the partial inhibitory concentration index (FIC) (FIC≤0.5, indicating that the two drugs were synergistic; 0.52, indicating that the two drugs are antagonistic), and acco...

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Abstract

The invention provides a frangipani flower extract and a preparation method and application thereof, and specifically discloses a frangipani flower water extract, the frangipani flower water extract is obtained by the following method, and the frangipani flower medicinal material is crushed and extracted by water reflux, After filtering the reflux liquid, the solvent was removed to obtain frangipani water extract. It is also disclosed that the above-mentioned Frangipani water extract is used in the preparation of Stenotrophomonas maltophilia inhibitors, anti-Steophagomonas maltophilia disinfectants or drugs against Stenotrophomonas maltophilia or drugs against Stenotrophomonas maltophilia. Use in antibiotic sensitization of bacteria. The product prepared by the invention has a simple preparation method, does not need an organic solvent, is environmentally friendly, and can be applied to the pharmaceutical field.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a new application of Jasminum grandiflorum L. water extract. Background technique [0002] Stenotrophomonas maltophilia is a class of obligate aerobic, non-fermenting sugar non-fermenting Gram-negative bacilli. In recent years, due to the widespread use of broad-spectrum antibiotics and various invasive procedures that have led to an imbalance in the micro-ecology of the body, Stenotrophomonas maltophilia has become one of the important pathogens of nosocomial infections, and it is closely related to blood infections, meningitis, pneumonia, urinary tract infections, etc. Infection and surgical site infection are closely related. According to reports, from 2010 to 2019, a total of 4077 strains of non-fermenting Gram-negative bacilli were detected in clinical specimens of a hospital, and the detection rate of Stenotrophomonas maltophilia was as high as 10.96%. Due t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/63A61K45/06A61P31/04A23L33/105A61K133/00
CPCA61K36/63A61K45/06A61P31/04A23L33/105A23V2002/00A61K2236/331A61K2236/51A23V2200/30A61K2300/00
Inventor 魏凤环卢静雅芮勇宇叶佩雯曾小燕罗超华
Owner SOUTHERN MEDICAL UNIVERSITY
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