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Biological probe sample application method and process based on high-density dot matrix

A biological probe and high-density technology, applied in the field of life sciences, can solve the problems of reagent mixing, lower efficiency, waste of raw materials, etc., and achieve the effect of avoiding reagent mixing, reducing error rate, and increasing sample density

Pending Publication Date: 2021-08-31
江西烈冰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional contact spotting process not only requires repeated sampling, wastes raw materials and reduces efficiency, but also requires frequent cleaning of the spotting head when spotting different types of samples, and it is difficult to completely avoid the possibility of reagent contamination

Method used

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  • Biological probe sample application method and process based on high-density dot matrix
  • Biological probe sample application method and process based on high-density dot matrix
  • Biological probe sample application method and process based on high-density dot matrix

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A high-density lattice-based bioprobe spotting method and process, specifically comprising the following steps:

[0028] Step 1. According to the optimal microfluidic properties of the droplet of liquid solvent and the efficiency of biochemical coupling between the sample and the substrate, select the appropriate concentration of printbuffer, dissolve the probe and sample in the liquid solvent, and distribute it to a 96-well plate (384-well plate), and place the orifice plate on the sample stage;

[0029] Step 2. Select epoxy silane coating substrate or N-hydroxysuccinimide ester coating according to the connection firmness, connection efficiency, and marginal diffusion range of the coated substrate to the sample and substrate, combined with actual production requirements substrates, and place the coated substrates on the substrate table in order, and number the positions of the coated substrates according to the numbering method. The specific numbering method is as fol...

Embodiment 2

[0038] A DNA high-density array biochip production process, including: dissolving Oligo probes with 5'-biotin modification and 3'-Cy5 modification in printbuffer, and distributing them to 96-well plates according to requirements, each well Add 10 μL of sample into the sample; place the 96-well plate filled with samples on the sample stage; place 100 N-hydroxysuccinimide ester-coated chips on the substrate stage in sequence; start the non-contact spraying method. The central control computer of the foam device, open the corresponding software on the central control computer, input the production instructions, and the instructions are as follows: the number of substrates: 100, the number of droplets (volume / droplets) per spray: 40pL / 1, point Spacing (X): 150μm, dot spacing (Y): 150μm, repeated spray times: 16, dot matrix layout shape: rectangle, dot matrix area: 10.3mm×10.0mm, spray pattern: 8; non-contact spray Power on the spray device and adjust the spray environment. When th...

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Abstract

The invention discloses a biological probe sample application method and process based on a high-density dot matrix, wherein the method specifically comprises the following steps: a sample is prepared, the sample is distributed into a pore plate, and the pore plate is placed on a sample table; coating substrates are selected and placed on a substrate table in sequence; a foam spraying instruction is input into a control device of a non-contact foam spraying device, and the non-contact foam spraying device sucks a liquid sample in the pore plate and then moves to the position above the substrate table; non-contact foam spraying is carried out at the position 80 [mu]m above the substrates, movement is carried out above the substrates according to the instruction, and a dot matrix layout shape instruction is achieved; after foam spraying of the same sample is completed, the non-contact foam spraying device is cleaned, and the foam spraying task is repeated until the dot matrix layout of all the set substrates is completed; after the dot matrix layout is completed, all the substrates are dried and sealed; and finally sampling fluorescence detection is carried out on the substrates. According to the method, non-contact foam spraying is adopted, so that the sample application efficiency is effectively improved, the problem of reagent mixed dyeing is avoided to a great extent, and the sample application density is greatly improved.

Description

technical field [0001] The invention relates to the technical field of life sciences, in particular to a high-density lattice-based biological probe spotting method and process. Background technique [0002] Biochip technology originated from the hybridization of nucleic acid molecules. The so-called biochip generally refers to micro-array hybrid chips (micro-arrays) of biological information molecules (such as gene fragments, DNA fragments or polypeptides, proteins, sugar molecules, tissues, etc.) immobilized on a mutual support medium at high density. The sequence and position of each molecule are known, and it is a pre-set sequence lattice. Biochip (biochip or bioarray) integrates the biochemical analysis process on the surface of the chip based on the principle of specific interaction between biomolecules, so as to realize high-throughput and rapid detection of DNA, RNA, peptides, proteins and other biological components. Nowadays, biochips play an important role in me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/2813
Inventor 陈岱王超刘志刚
Owner 江西烈冰生物科技有限公司
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