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Detection method for colorectal cancer mismatch repair protein expression based on Raman spectrum

A Raman spectroscopy, colorectal cancer technology, applied in the field of medical detection, can solve the problems of complex process, high cost, long time-consuming, etc., and achieve the effect of simple process, cost saving and short time-consuming

Pending Publication Date: 2021-08-31
JILIN UNIV
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Problems solved by technology

The current method has the disadvantages of complex process, cumbersome, time-consuming and high cost

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  • Detection method for colorectal cancer mismatch repair protein expression based on Raman spectrum
  • Detection method for colorectal cancer mismatch repair protein expression based on Raman spectrum

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Embodiment Construction

[0025] Hereinafter, in conjunction with the accompanying drawings and the specific embodiments, make further description of the invention:

[0026] See Figure 1-2 , According to one embodiment of the present invention method for detecting protein expression in colorectal cancer based on the Raman spectrum of the error mismatch repair, includes the following steps:

[0027] (1) Fresh from body tissue of colorectal cancer, about the size of 2.0cm * 2.0cm, frozen in liquid nitrogen for 10-20 seconds.

[0028] (2) Take frozen tissue was cut with a thickness of approximately constant freezing microtome sections of 5um, take two.

[0029] (3) wherein a take placed confocal Raman spectrometer detector, the detector 100 of each tissue site, the corresponding Raman shift spectrum of cosmic ray spectrum de, filtering correction.

[0030] (4) the first sheet of the two immediately adjacent tissues by immunohistochemistry step, respectively corresponding mismatch repair protein expression by ...

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Abstract

The invention discloses a detection method for the colorectal cancer mismatch repair protein expression based on a Raman spectrum. The method comprises the steps of quickly freezing a to-be-detected colorectal cancer tissue, slicing into two adjacent slices, fixing on a glass slide, and carrying out single-point test and imaging test by a confocal Raman spectrometer to obtain the original Raman spectrum displacement data; meanwhile, fixing and carrying out immunohistochemical staining on the other corresponding tissue to determine the mismatch repair protein expression condition of the tissue, obtaining the Raman shift data through the processing of a support vector machine, classifying according to a immunohistochemical staining result, establishing a database; according to the database classification basis, classifying the newly detected tissue through the Raman spectrum detection result, and then judging the expression condition of the corresponding tissue mismatch repair protein. According to the detection method, the tissue sample treatment process is simple and convenient, no reagent is consumed in the later period, detection is rapid, and the method is simple and convenient, and is high in precision and low in cost.

Description

Technical field [0001] The present invention relates to the technical field of medical testing, in particular, to a method for detecting protein expression in colorectal cancer based on the Raman spectrum of the error mismatch repair. Background technique [0002] Of colorectal cancer (Colorectal cancer, CRC) is one of the high incidence of human cancer, its incidence has been rising, the world's cases of colorectal cancer each year suffering from colorectal over 100 million people, it has risen to third in the world in the incidence of malignant tumors position, its mortality rate ranks second in cancer cause of death. In my country, colorectal cancer incidence and mortality rates have maintained an upward trend, the incidence rate of increase is twice the world average, the incidence of cancer is already in third place, serious harm to human health. Currently, colorectal cancer treatments to surgical treatment, combined with systemic or local chemotherapy, radiation therapy and...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N1/42G01N1/28G01N1/06G06K9/62
CPCG01N21/65G01N1/42G01N1/286G01N1/06G01N2001/2873G06F18/2411
Inventor 宋德锋高永建邢健鹏冯野
Owner JILIN UNIV
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