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BOD detection device and BOD detection method

A detection system and detection chip technology, applied in the direction of sterilization methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of high technical requirements, poor reproducibility of results, long detection time of biochemical methods, etc.

Active Publication Date: 2021-09-03
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional biochemical method has high technical requirements and poor reproducibility due to the long detection time and complicated operation

Method used

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  • BOD detection device and BOD detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] A device and method for illustrating a BOD detection provided by the present invention

[0079] Such as figure 1 Shown, the device that the BOD detection that the present invention provides comprises:

[0080] A-microbial circulation culture system, B-constant temperature system, C-detection system, D-waste liquid collection system, 1-sample liquid supply device, 2-three-way valve, 3-microfluidic chip, 4-heating device (polymer Imide heating film), 5-multi-channel peristaltic pump, 6-thermostatic controller, 7-multi-channel valve, 8-flushing liquid reservoir, 9-detection chip, 10-waste liquid peristaltic pump, 11-waste liquid container , 12-data collector, 13-microfluidic chip inlet, 14-serpentine channel, 15-microfluidic chip outlet, 16-detection electrode, 17-detection channel.

[0081] Among them, the microfluidic chip is made of glass chip, the size is 5×10cm, the width of the serpentine channel is 1mm, and the depth is 500μm. The joints of the pipeline and each p...

Embodiment 2

[0093]The standard value of BOD5 was detected respectively according to the above-mentioned standard method of BOD5 and the method of Example 1, wherein, the difference was that the sample liquid used was taken from the water body in the river. The results are shown in Table 3.

[0094] table 3

[0095] BOD5 mg / L (standard method) 2.5 BOD5 mg / L (the method of embodiment 2) 2.3

[0096] As can be seen from the data in table 3, the value of BOD measured by the method of the present invention and the result obtained by the standard method of BOD detecting the water body in the river are less different, and the method of the present invention is used to measure the BOD in the real water body. The results obtained are reliable.

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Abstract

The invention relates to the technical field of water environment chemical analysis, and particularly relates to a BOD detection device and a BOD detection method. The device comprises a microorganism circulating culture system (A), a constant temperature system (B) and a detection system (C), wherein the microorganism circulating culture system (A) comprises a micro-fluidic chip (3), and a zigzag channel is formed in the micro-fluidic chip (3); the constant temperature system (B) is located below the microorganism circulating culture system (A) and used for controlling the temperature of the microorganism circulating culture system (A). The device can increase the culture temperature of a sample solution, improve the fluidity of the sample solution and accelerate an oxygen consumption reaction, so that the BOD detection time is shortened; and besides, the device is simple to operate, convenient to maintain, wide in application range, long in continuous working time and capable of realizing online full-automatic flowing culture and detection.

Description

technical field [0001] The invention relates to the technical field of water environment chemical analysis, in particular to a BOD detection device and a BOD detection method. Background technique [0002] Biochemical Oxygen Demand (Biochemical Oxygen Demand, referred to as BOD) refers to the amount of dissolved oxygen (DO) that microorganisms need to consume when they decompose organic matter in a certain volume of water through metabolism within a certain period of time. quantity. This indicator can indirectly indicate the degree of water body pollution by organic matter, expressed in mg / L or ppm, and is one of the most important water quality detection indicators in environmental pollution monitoring. It is mainly used to monitor the pollution status of natural water bodies such as reservoirs, lakes, and rivers. . [0003] The traditional biochemical method has high technical requirements and poor reproducibility due to the long detection time and complicated operation....

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/34C12M1/02C12M1/00B01L3/00
CPCC12M23/16C12M23/58C12M29/00C12M37/04C12M41/22C12M41/32B01L3/5027B01L2200/10
Inventor 林玲李楠
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY