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Bod detection device and bod detection method

A detection method and detection device technology, applied in the direction of sterilization methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of long detection time, poor result reproducibility, and high technical requirements of biochemical methods, and achieve shortening The effect of BOD detection time, good reproducibility and simple operation

Active Publication Date: 2022-04-08
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The traditional biochemical method has high technical requirements and poor reproducibility due to the long detection time and complicated operation

Method used

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Experimental program
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Effect test

Embodiment 1

[0078] A device and method for illustrating a BOD detection provided by the present invention

[0079] Such as figure 1 Shown, the device that the BOD detection that the present invention provides comprises:

[0080] A-microbial circulation culture system, B-constant temperature system, C-detection system, D-waste liquid collection system, 1-sample liquid supply device, 2-three-way valve, 3-microfluidic chip, 4-heating device (polymer Imide heating film), 5-multi-channel peristaltic pump, 6-thermostatic controller, 7-multi-channel valve, 8-flushing liquid reservoir, 9-detection chip, 10-waste liquid peristaltic pump, 11-waste liquid container , 12-data collector, 13-microfluidic chip inlet, 14-serpentine channel, 15-microfluidic chip outlet, 16-detection electrode, 17-detection channel.

[0081] Among them, the microfluidic chip is made of glass chip, the size is 5×10cm, the width of the serpentine channel is 1mm, and the depth is 500μm. The joints of the pipeline and each p...

Embodiment 2

[0093]The standard value of BOD5 was detected respectively according to the above-mentioned standard method of BOD5 and the method of Example 1, wherein, the difference was that the sample liquid used was taken from the water body in the river. The results are shown in Table 3.

[0094] table 3

[0095] BOD5 mg / L (standard method) 2.5 BOD5 mg / L (the method of embodiment 2) 2.3

[0096] As can be seen from the data in table 3, the value of BOD measured by the method of the present invention and the result obtained by the standard method of BOD detecting the water body in the river are less different, and the method of the present invention is used to measure the BOD in the real water body. The results obtained are reliable.

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Abstract

The invention relates to the technical field of water environment chemical analysis, in particular to a BOD detection device and a BOD detection method. The device comprises: a microorganism circulation culture system (A), a constant temperature system (B) and a detection system (C); wherein, the microorganism circulation culture system (A) comprises a microfluidic chip (3), and the microfluidic chip (3) is provided with a zigzag channel; wherein, the constant temperature system (B) is located below the microorganism circulation culture system (A), and is used for temperature control of the microorganism circulation culture system (A). The device can increase the temperature of the sample liquid culture, increase the fluidity of the sample liquid, accelerate the oxygen consumption reaction, thereby shortening the BOD detection time, and is simple to operate, easy to maintain, has a wide range of applications, and has a long continuous working time. It can realize online automatic flow culture and detection.

Description

technical field [0001] The invention relates to the technical field of water environment chemical analysis, in particular to a BOD detection device and a BOD detection method. Background technique [0002] Biochemical Oxygen Demand (Biochemical Oxygen Demand, referred to as BOD) refers to the amount of dissolved oxygen (DO) that microorganisms need to consume when they decompose organic matter in a certain volume of water through metabolism within a certain period of time. quantity. This indicator can indirectly indicate the degree of water body pollution by organic matter, expressed in mg / L or ppm, and is one of the most important water quality detection indicators in environmental pollution monitoring. It is mainly used to monitor the pollution status of natural water bodies such as reservoirs, lakes, and rivers. . [0003] The traditional biochemical method has high technical requirements and poor reproducibility due to the long detection time and complicated operation....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/38C12M1/34C12M1/02C12M1/00B01L3/00
CPCC12M23/16C12M23/58C12M29/00C12M37/04C12M41/22C12M41/32B01L3/5027B01L2200/10
Inventor 林玲李楠
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY