Preparation method and application of antibacterial metal nano-enzyme OA-MnO2

Abstract

The invention belongs to the technical field of metal nano materials, and particularly discloses a preparation method and application of antibacterial metal nano-enzyme OA-MnO2. The manganese dioxide (OA-MnO2) nano-enzyme is synthesized in one pot through an oleic acid (OA) nano-emulsion template method. The nano-enzyme has good oxidase activity and is capable of generating reactive oxygen species (ROS) causing damage to microbial cells, so that the gram-positive (staphylococcus aureus) and gram-negative (escherichia coli) bacterial strains can be effectively killed, and the nano-enzyme has a good biofilm prevention and damage ability on staphylococcus aureus. The antibacterial metal nano-enzyme OA-MnO2 prepared by the preparation method disclosed by the invention shows relatively high stability and biocompatibility under physiological conditions, is superior to vancomycin in in-vivo antibacterial performance, and is a safe and effective tool for resisting bacterial infection and promoting wound healing.

Description

technical field [0001] The invention belongs to the technical field of metal nanomaterials, in particular to an antibacterial metal nanozyme OA-MnO 2 preparation methods and applications. Background technique [0002] Natural enzymes have strong catalytic activity under mild conditions, and exist in various processes of organism life activities, such as: tricarboxylic acid cycle, glycolysis, synthesis and metabolism of lipids, proteins and amino acids, etc., most of the natural enzymes The main component of the enzyme is protein, which has strict requirements on the reaction environment. The protein is easily denatured in the environment of overacidity, overalkaline, overcooling and overheating, and the catalytic activity of the enzyme is also lost. As a result, artificial mimetic enzymes have been produced, and metal nanozymes are one of them. [0003] Nanozymes are nanomaterials capable of catalyzing biological substrates with reaction kinetics similar to natural enzymes...

AI Technical Summary

Problems solved by technology

[0004] Although nanozymes have made great progress, the low catalytic activity and poor stability limit the development of artificial enzymes of nanomaterials. The poor stability is mainly due to the uneven distribution of nanoparticles and easy aggregation. The low catalytic activity is mainly due to the impact on the catalytic environment. There are certain requirements, for example: gold nanoparticles exhibit inherent catalase-like catalytic activity only under alkaline conditions, while the microenvironment of the bacterial infection site is weakly acidic

In addition, the preparation of stable nanomaterials often requires relatively complex steps, which limit the further application of nanozymes in medical science.

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