Kocuria rhizophila and application thereof
A technology for Cockroaches, strains, applied in the direction of bacteria, microorganisms, microorganism-based methods, etc., to achieve excellent fermentation characteristics, ensure consistency, and improve the effect of flavor
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[0022] 1. Screening, isolation, purification and identification of strains
[0023] Under sterile conditions, take a sample from the center of the commercially available Nuodeng ham, homogenize the sample in sterile saline and make appropriate dilutions, and spread the diluted solution on a casein agar plate (1% casein, 0.3% beef powder , sodium chloride 0.5%, dipotassium hydrogen phosphate 0.2%, agar 1.5%, pH value 7.4±0.2), and cultivated at 37°C for 48h.
[0024] On the casein agar plate, select the colony with a transparent circle around it, separate and purify it for more than 3 generations, and obtain several strains with proteolytic activity.
[0025] After testing the nitrate reducing ability and catalase activity of these strains, a strain AP1 with good catalase activity, nitrate reducing ability and protein decomposition ability was selected, and it was positive by Gram reagent staining. ; The strain was identified by 16S rDNA sequence as Corkia rhizophila, belongin...
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