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Preparation method and application of bionic nano-carrier for reducing excessive calcium ions in tumors

A bionic nanometer and calcium ion technology, which is applied in the direction of antineoplastic drugs, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc.

Active Publication Date: 2021-09-14
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, accumulation of these toxic antineoplastic agents in other organs is still unavoidable, resulting in damage to healthy tissue

Method used

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  • Preparation method and application of bionic nano-carrier for reducing excessive calcium ions in tumors
  • Preparation method and application of bionic nano-carrier for reducing excessive calcium ions in tumors
  • Preparation method and application of bionic nano-carrier for reducing excessive calcium ions in tumors

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preparation example Construction

[0046] The preparation method of biomimetic nanoparticles for reducing excess calcium ions in tumors of the present invention includes the following steps, but is not limited to the following steps:

[0047] (1) First, gold nanoparticles are prepared as the core of the biomimetic nano-carrier by a simple hydrothermal method using chloroauric acid solution and adding a reducing agent; the specific process is as follows: add 0.01mol / L chloroauric acid solution to the ultrapure water, heated to a boil. After that, a 0.01 mol / L sodium citrate solution was added to the boiling mixed solution. Continue heating, and react for 10 to 120 minutes in a boiling state, the solution becomes light red, and a gold nanoparticle solution is obtained;

[0048] The volume ratio of the chloroauric acid solution, ultrapure water and sodium citrate solution is 1:20:2˜1:40:2.

[0049] (2) The core of the gold nanoparticle is modified by gold-sulfhydryl bond and amidation reaction to capture the she...

Embodiment 1

[0064] Preparation of biomimetic nanoparticles (AEP NPs) with EGTA as capture shell and gold nanoparticles as core:

[0065] (1) 376 mg of chloroauric acid trihydrate was dissolved in 100 mL of ultrapure water to obtain a chloroauric acid solution (0.01 mol / L). 258 mg of sodium citrate was dissolved in 100 mL of ultrapure water to obtain a sodium citrate solution (0.01 mol / L). Add 15mL of the above chloroauric acid solution to 510mL of ultrapure water, and heat to boiling. After that, 30 mL of the above sodium citrate solution (0.01 mol / L) was taken and added to the boiling mixed solution. Continue heating and react in boiling state for 10 minutes, the solution turns light red, stop heating to obtain gold nanoparticles (AuNPs) solution;

[0066] (2) 192.8 mg of mercaptoethylamine was dissolved in 100 mL of ultrapure water to obtain a mercaptoethylamine solution (0.025 mol / L). Take 5 mL of mercaptoethylamine solution and slowly add to 500 mL of gold nanoparticle solution. A...

Embodiment 2

[0070] Preparation of biomimetic nanoparticles (AEPF NPs) with folic acid-polyethylene glycol (molecular weight: 2000) as the crown, EGTA as the capture shell and gold nanoparticles as the core:

[0071](1) 376 mg of chloroauric acid trihydrate was dissolved in 100 mL of ultrapure water to obtain a chloroauric acid solution (0.01 mol / L). 258 mg of sodium citrate was dissolved in 100 mL of ultrapure water to obtain a sodium citrate solution (0.01 mol / L). Add 15mL of the above chloroauric acid solution to 510mL of ultrapure water, and heat to boiling. After that, 30 mL of the above sodium citrate solution (0.01 mol / L) was taken and added to the boiling mixed solution. Continue heating and react in boiling state for 10 minutes, the solution turns light red, stop heating to obtain gold nanoparticles (AuNPs) solution;

[0072] (2) 192.8 mg of mercaptoethylamine was dissolved in 100 mL of ultrapure water to obtain a mercaptoethylamine solution (0.025 mol / L). Take 5 mL of mercapto...

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Abstract

The invention relates to a bionic nano-carrier for reducing excessive calcium ions in tumors and a preparation method and application thereof. The preparation method comprises the steps that a mixed solution of triethylamine and monobrominated polyethylene glycol is added into a gold nanoparticle solution modified with a calcium ion trapping agent, a reaction is conducted for 1-3 days under stirring, dialysis is conducted, and a bionic nanoparticle solution with the calcium ion trapping agent protected by polyethylene glycol is obtained; a mixed solution of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide, N-hydroxysuccinimide and a tumor targeting agent is added into the bionic nanoparticle solution with the calcium ion trapping agent protected by polyethylene glycol, a reaction is conducted for 1-3 days under stirring, dialysis is conducted, and the calcium-ion-trapped bionic nanoparticle solution with a tumor targeting function is obtained. The prepared bionic nano particles are good in biocompatibility, safe, non-toxic, excellent in stability and suitable for the medical field; and the choices of the calcium ion trapping agent and the tumor targeting agent used herein are wide-ranging, and the modification method is simple.

Description

technical field [0001] The invention relates to the fields of nanomaterial technology and biomedical materials, in particular to a preparation method and application of a bionic nanocarrier for reducing excess calcium ions in tumors. Background technique [0002] Cancer is one of the most malignant diseases threatening human health. Since the tumorigenic intracellular and extracellular microenvironment is a hotbed for tumor growth, tumors are difficult to cure. The regulation of these tumor-specific microenvironments is conducive to the efficient and precise treatment of tumors. [0003] In these tumorigenic microenvironments, overexpressed transmembrane calcium ion channel proteins and highly active calcium ion-related proteins will lead to an abnormal increase in calcium ion concentration in tumor cells, which will enhance cell invasiveness and the ability to absorb external nutrients , contributing to the malignant proliferation of tumors. In terms of related treatment,...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/10A61K47/02A61K31/198A61K31/223A61P35/00
CPCA61K9/5146A61K9/5115A61K31/198A61K31/223A61P35/00
Inventor 陈鑫郭晓燕袁萍耘于小倩刘涛
Owner XI AN JIAOTONG UNIV
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