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Erythromycin degrading bacterium IURM F61 and application thereof

A technology of erythromycin and degrading bacteria, applied in the biological field, can solve the problems of high cost of incineration method, low recovery rate of useful components, and unpleasant production volume.

Inactive Publication Date: 2021-09-14
YILI CHUANNING BIOTECH CO +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But these technologies all have many deficiencies, such as: the cost of the incineration method is too high, and the hazardous waste landfill technology is not suitable for disposal of erythromycin slag with a large amount of production, high moisture content and high organic matter content; Comprehensive utilization technologies such as culture medium, adsorbent, and extraction of useful components. Due to the low recycling rate of useful components, a large amount of remaining bacterial residues still need to be further disposed of.

Method used

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  • Erythromycin degrading bacterium IURM F61 and application thereof
  • Erythromycin degrading bacterium IURM F61 and application thereof
  • Erythromycin degrading bacterium IURM F61 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Cultivate the erythromycin-degrading bacteria IURM F61 in LB liquid medium, collect the bacteria by centrifugation, resuspend with sterile water, and dilute to OD 600 =1, inoculate 1mL of the selected strain into 100.0mL basic liquid inorganic salt medium containing 100mg erythromycin, place it in a constant temperature shaker at 180r / min, and culture it at 37°C for 120h. The degradation rate is 58.2%. The efficiency effect is obvious.

Embodiment 2

[0052] The erythromycin-degrading bacteria IURM F61 was inoculated in 100 g of sterilized soil at an inoculum amount of 1%, and 100 mg of erythromycin was added, and cultured at 37° C. for 120 h. The degradation rate was determined to be 47.91%.

Embodiment 3

[0054] The erythromycin-degrading bacteria IURM F61 was inoculated in 100 mL of sterilized erythromycin-containing wastewater at an inoculum size of 1%. The concentration of erythromycin in the wastewater was determined to be 130 mg / L. Under culture for 120h, the degradation rate of erythromycin was determined to be 51.35%.

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Abstract

The invention discloses an erythromycin degrading bacterium IURM F61 and application thereof, and belongs to the technical field of biology. Experiments prove that the erythromycin degrading bacterium IURM F61 can survive in an environment with erythromycin as a unique carbon source, and has very high erythromycin degrading activity. The erythromycin degrading bacterium IURM F61 can be applied to erythromycin degradation in a polluted environment, and has very good application value in the aspects of water treatment and soil remediation.

Description

technical field [0001] The invention belongs to the field of biological technology, in particular to an erythromycin-degrading bacterium IURM F61 and its application in the field of erythromycin-containing degradation in soil or waste water. Background technique [0002] Erythromycin is a secondary metabolite of microorganisms in the culture medium of erythromycin manufacturing industry. The erythromycin residue has high water content and high viscosity, and it is easy to autolyze and deteriorate after long-term storage. The fungus residue is rich in nutrients such as protein, amino acid, and mineral elements, and has good utilization value. Due to the high content of organic matter in the fungus residue, it can cause secondary fermentation, turn black in color, produce foul smell, and seriously affect the environment. Therefore, researchers have been actively seeking an economical and efficient treatment method for a long time. [0003] Erythromycin and its bacterial degra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34A62D3/02C12R1/05C02F101/38A62D101/28A62D101/26
CPCC12N1/20B09C1/10C02F3/34A62D3/02C02F2101/38A62D2101/28A62D2101/26
Inventor 邓留杰任建军邓旭衡沈云鹏李志杰董丽萍牛东泽刘淑云李春雨李真真
Owner YILI CHUANNING BIOTECH CO