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Preparation method of casein-gold nanocluster and application of casein-gold nanocluster in aureomycin detection

A technology of gold nanoclusters and casein, which is applied in the field of preparation of casein-gold nanoclusters, can solve the problems of bacterial resistance, low sensitivity, environmental pollution and the like

Inactive Publication Date: 2021-09-14
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Chlortetracycline is a broad-spectrum tetracycline antibiotic that can inhibit both Gram-positive and Gram-negative bacteria. Improper, residues in animal meat, eggs, milk and other foods have caused serious side effects, such as environmental pollution, bacterial resistance, etc.
However, the traditional detection method for aureomycin has limitations such as poor stability, low sensitivity, and long time consumption, so it is urgent to develop a fast and efficient detection method

Method used

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  • Preparation method of casein-gold nanocluster and application of casein-gold nanocluster in aureomycin detection
  • Preparation method of casein-gold nanocluster and application of casein-gold nanocluster in aureomycin detection
  • Preparation method of casein-gold nanocluster and application of casein-gold nanocluster in aureomycin detection

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preparation example Construction

[0026] The present invention provides the following technical scheme: a method for preparing casein-gold nanoclusters, comprising the following steps:

[0027] Weigh a certain amount of casein, add 750 μL of water to dissolve, add sodium hydroxide solution, and heat for 20 minutes in a water bath; add chloroauric acid to the above solution, and heat for 0 minutes in a water bath; use a fluorescence spectrometer to detect the excitation spectrum of the product and ll. Increase the reaction time and observe the fluorescence spectra of different reaction times. As the reaction time increases, the fluorescence intensity gradually increases. The reaction time is 3 hours and the fluorescence intensity reaches the strongest; therefore, 3 hours is selected as the best time for preparing casein-gold nanocluster Reaction time.

[0028] Weigh 5 mg of casein, add 750 μL of water to dissolve, add sodium hydroxide solution, and heat in a water bath for 20 minutes; add chloroauric acid to t...

Embodiment 1

[0033] Example 1: Weigh 30 mg of casein, add 750 μL of water to dissolve; second step, add 50 μL of 3M sodium hydroxide, and react in a water bath at 70°C for 20 minutes; third step, add 300 μL of 10 mM sodium hydroxide Chlorauric acid was added to the above solution, and heated in a water bath at 70° C. for 1 hour to obtain casein-gold nanoclusters.

Embodiment 2

[0034] Example 2: Weigh 30 mg of casein, add 750 μL of water to dissolve; the second step, add 50 μL of 3M sodium hydroxide, and react in a water bath at 70°C for 20 minutes; third step, add 300 μL of 10 mM sodium hydroxide Chlorauric acid was added to the above solution, and heated in a water bath at 70°C for 2 hours to obtain casein-gold nanoclusters.

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Abstract

The invention discloses a preparation method of a casein-gold nanocluster and application of the casein-gold nanocluster in aureomycin detection. The preparation method comprises the following specific steps of S1, weighing a certain amount of casein, adding 750 microliters of water for dissolution, then adding a sodium hydroxide solution for adjusting the pH of the solution, and conducting heating for 15-20 minutes in a water bath at the temperature of 37-90 DEG C, so as to obtain a solution A; S2, adding chloroauric acid into the solution A obtained in the step S1, and conducting heating in a water bath at 37-90 DEG C for 0-5 hours to obtain a solution B; and S3, storing the solution B obtained in the step 2 under the condition of 4 DEG C to obtain the casein-gold nanocluster. The fluorescent gold nano-cluster has unique photophysical characteristics, is simple to prepare, almost non-toxic and excellent in biocompatibility, the aureomycin detection method is rapid and simple, the detection sensitivity is high, the detection limit is low, and the fluorescent gold nano-cluster is an ideal fluorescent nano-material applied to the fields of biology and medicine.

Description

technical field [0001] The invention relates to the technical field of synthesis of gold nano-clusters, in particular to a preparation method of casein-gold nano-clusters and its application in the detection of aureomycin. Background technique [0002] Casein is a phosphorus-calcium-binding protein, an amphiphilic copolymer of both hydrophilic and hydrophobic amino acid residues. For young children, casein is a source of amino acids as well as calcium and phosphorus. At the same time, casein has many physiological effects such as prevention and treatment of osteoporosis and rickets, regulation of blood pressure, treatment of iron-deficiency anemia, magnesium-deficiency neuritis, etc. Efficient absorption. Casein has a variety of amino acids, especially a large number of proline residues, so it is expected to be a template for the synthesis of gold nanoclusters. [0003] Metal nanoclusters have molecular-like properties, can be photofluorescent, have good stability, mild s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/31G01N33/53G01N1/38
CPCG01N21/6402G01N21/6428G01N21/31G01N33/53G01N1/38
Inventor 付丁伊曹蕾袁心语周梦艳丁姝姝
Owner NANTONG UNIVERSITY
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