Method and device for antibody humanization based on dynamic programming

A dynamic programming and humanization technology, applied in the field of antibody humanization, can solve the problems of reducing antibody affinity and loss of specific target affinity, etc.

Pending Publication Date: 2021-09-14
SUZHOU CUREMED BIOMEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually, the human framework with the highest homology to the non-human antibody framework region is selected as the recipient of CDR grafting. The main problem with this method is the loss of affinity for its specific target
In some cases, grafting the CDR loops of murine antibodies directly onto the human framework did not affect antibody affinity, but in many more cases, it greatly reduced antibody affinity

Method used

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  • Method and device for antibody humanization based on dynamic programming
  • Method and device for antibody humanization based on dynamic programming
  • Method and device for antibody humanization based on dynamic programming

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Experimental program
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Embodiment 1

[0042] like figure 1 The method for antibody humanization based on dynamic programming provided by an embodiment of the present disclosure is shown, including the following steps:

[0043] S1: Obtain the human antibody gene sequence;

[0044] Specifically, fasta files encoding V, D, J and C gene sequences of human antibodies can be downloaded from the IMGT database.

[0045] S2: number the heterologous antibody gene sequence;

[0046] Specifically, the Kabat numbering scheme is used for numbering the heterologous antibody gene sequences.

[0047] S3: Align the heterologous antibody gene sequence with the human antibody gene sequence based on the dynamic rule algorithm;

[0048] S4: According to the comparison result, amino acid substitution is performed to obtain a humanized antibody gene sequence.

[0049] In an alternative embodiment, reference is made to the description attached figure 2 , in step S3, comparing the heterologous antibody gene sequence with the human an...

Embodiment 2

[0082] The present disclosure also provides a device for antibody humanization based on dynamic programming, comprising,

[0083] The human antibody gene sequence acquisition module is used to obtain the human antibody gene sequence;

[0084] The numbering module is used to number the heterologous antibody gene sequence;

[0085] The alignment module is used to compare the heterologous antibody gene sequence with the human antibody gene sequence based on the dynamic rule algorithm;

[0086] The amino acid replacement module is used to perform amino acid replacement according to the comparison result to obtain the humanized antibody gene sequence.

[0087] In an optional embodiment, in the human antibody gene sequence acquisition module, the human antibody gene sequences are V, D, J and C gene sequences of human antibodies.

[0088] In an alternative embodiment, in the numbering module, the Kabat numbering scheme is used for numbering the heterologous antibody gene sequences....

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Abstract

The invention provides a method and a device for antibody humanization based on dynamic programming. The method comprises the following steps: acquiring a human antibody gene sequence; numbering gene sequences of the heterologous antibody; on the basis of a dynamic rule algorithm, comparing the heterologous antibody gene sequence with a human antibody gene sequence; and according to the comparison result, carrying out amino acid replacement to obtain a humanized antibody gene sequence. According to the antibody humanization method and device based on dynamic programming, humanization transformation of amino acid residues on the surface of the heterologous antibody is carried out through dynamic programming, the heterology of the antibody is reduced, the humanization speed is high, and sequence humanization can be carried out in batches. In addition, the humanized antibody sequence has good affinity and specificity.

Description

technical field [0001] The present disclosure relates to the technical field of antibody humanization, and in particular, to a method and device for antibody humanization based on dynamic programming. Background technique [0002] At present, antibodies have become effective tools for the treatment and diagnosis of various human diseases. However, non-human antibodies can induce a human immune response, resulting in neutralization of the administered antibody, and limit the use of such antibodies in the treatment of human disease. To overcome this problem, antibody humanization technology was developed. Antibody humanization is an effective method to eliminate or reduce the immunogenicity of these antibodies. To date, researchers have innovated various methods to humanize non-human antibodies and improve their affinity, specificity, and other properties. [0003] A common method of humanization of non-human antibodies is complementarity determining region (CDR) grafting, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B30/00G16B30/10
CPCG16B30/00G16B30/10
Inventor 侯强波左炽健孙振华
Owner SUZHOU CUREMED BIOMEDICAL TECH CO LTD
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