Application of pleurotus geesteranus polysaccharide in preparation of immunological enhancement medicine
An immune-enhancing and xiuzhen mushroom technology is applied in the directions of drug combination, pharmaceutical formula, medical preparations containing active ingredients, etc. It can solve the problem of not finding the immune activity of xiuzhen mushroom polysaccharide, few and other problems, and achieve the improvement of body weight and immune organ index. , Relieve damage, enhance the effect of antioxidant capacity
Pending Publication Date: 2021-09-17
HENAN UNIVERSITY
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Problems solved by technology
The researches on xiuzhen mushrooms mainly focus on the cultivation, fresh-keeping and biological characteristics of strains, but there are few research reports on the biological activities of polysaccharides, which mainly focus on anti-tumor, blood fat-lowering, anti-aging and liver-protecting effects. There are researches and reports on the immune activity of Pleurotus chinensis polysaccharides
Method used
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Embodiment 1
[0021] In the following application tests, the phlox polysaccharide PGp used was prepared through the following steps:
[0022] Take 930 g of the fruiting body powder of Chrysanthemum officinalis, and use petroleum ether to degrease twice at room temperature. The amount of petroleum ether added in the two times is 2000 mL and 1500 mL respectively, and the extraction time is 3 days each time. Filter after leaching, and dry the filter residue in the shade to obtain 900 g of pear mushroom powder residue. Then, the powder residue of Pleurotus osmanthus was leached twice with 75% ethanol at room temperature to remove small molecules. The amount of 75% ethanol added twice was 2000 mL and 1500 mL respectively, and the leaching time was 3 days each time. After leaching, filter and evaporate ethanol to obtain 820 g of dried mushroom powder. According to the ratio of solid to liquid: 1 g: 30 mL, direct drinking water was added to the residue, and it was decocted three times at 85°C, ea...
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The invention discloses application of pleurotus geesteranus polysaccharide in preparation of an immunological enhancement medicine. The application comprises the steps that cyclophosphamide is used for establishing a mouse hypoimmunity model, and the body weight and immune organ indexes of a mouse are measured; a nitric oxide kit is used for determining the content of NO, and an ELISA kit is used for determining the content of cell factors such as IL-2, IL-4, IL-6 and TNF-alpha; the activity of lactic dehydrogenase and acid phosphatase is determined by using LDH and ACP kits; antioxidant indexes are determined by using MDA, SOD and other kits; and the mouse spleen tissue morphology is analyzed by an HE staining method. Research results show that the pleurotus geesteranus polysaccharide PGp can improve the body weight and the immune organ index of the mouse, remarkably increase the content of cell factors such as IL-2, IL-4, IL-6 and TNF-alpha of the mouse, and effectively enhance the oxidation resistance and remarkably relieve damage to immune organs of the mouse; and meanwhile, the loss of the immune organs of the mouse can be obviously relieved, the pleurotus geesteranus polysaccharide PGp can be used for preparing immunomodulators, and a certain development value is achieved for new immune drugs.
Description
Technical field: [0001] The invention belongs to the technical field of polysaccharide medicines, and in particular relates to the application of Pleurotus chinensis polysaccharides with immunoregulatory effects in the preparation of immune enhancing medicines. Background technique: [0002] The immune system (Immune system) is the most important system for the body to perform immune response and immune function. It has the functions of recognizing and clearing the body's antigens, coordinating with other systems of the body, and jointly maintaining the stability and physiological balance of the body's internal environment. Immunosuppression refers to the suppression of immune responses. Due to congenital deficiency or acquired malnutrition, many people's immune function against various chronic diseases is constantly weakened, leading to the occurrence of various diseases, such as upper respiratory tract infection, urinary tract infection, sepsis and meningitis Wait. Theref...
Claims
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Login to View More IPC IPC(8): A61K31/715A61K36/07A61P37/04A61P39/06C08B37/00
CPCA61K31/715A61K36/07A61P37/04A61P39/06C08B37/0003
Inventor 康文艺张岩马常阳梁振华王莉李昌勤
Owner HENAN UNIVERSITY