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Lactobacillus reuteri CCFM1178 capable of intervening in metabolic syndrome, and application of lactobacillus reuteri CCFM1178

A technology of CCFM1178, Lactobacillus reuteri, applied in the field of microbiology, can solve the problem of the lack of research on probiotics in the colon intestinal barrier, and achieve the goal of restoring the imbalance of intestinal flora, reducing fasting blood sugar levels, and reducing glucose tolerance. Effect

Active Publication Date: 2021-09-17
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above patents all use probiotics to alleviate metabolic syndrome, and there are few studies on how probiotics can inhibit endotoxin and protect the intestinal barrier of the colon.

Method used

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  • Lactobacillus reuteri CCFM1178 capable of intervening in metabolic syndrome, and application of lactobacillus reuteri CCFM1178
  • Lactobacillus reuteri CCFM1178 capable of intervening in metabolic syndrome, and application of lactobacillus reuteri CCFM1178
  • Lactobacillus reuteri CCFM1178 capable of intervening in metabolic syndrome, and application of lactobacillus reuteri CCFM1178

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Example 1: Screening and identification of Lactobacillus reuteri CCFM1178

[0071] (1) Isolation and screening of lactic acid bacteria

[0072] (1) Take 1g of pig feces from Lijiang City, Yunnan Province. After gradient dilution, it is spread on a plate of highly selective solid medium for Lactobacillus (each 1L MRS medium contains 0.02g vancomycin, 20g agar, pH value is 5.0±0.1), and cultured at 37°C for 48-72 hours.

[0073] (2) Observe and record the colony morphology, pick and streak the colonies for purification.

[0074] (3) Cultivate in MRS liquid medium at 37° C. for 24 hours, perform Gram staining on the obtained colonies, and record the colony morphology.

[0075] (4) Discard the Gram-negative bacterial strains and Gram-positive cocci in the colonies, and select the Gram-positive bacilli.

[0076] (5) After the catalase analysis, discard the catalase-positive strains and keep the catalase-negative strains.

[0077] (2) Molecular biological identification o...

Embodiment 2

[0090] Example 2: Tolerance of Lactobacillus reuteri CCFM1178 to simulated gastrointestinal fluid

[0091] Streak-inoculate the cryopreserved Lactobacillus reuteri CCFM1178 in MRS solid medium (MRS medium + 0.05% cysteine ​​hydrochloride), anaerobic static culture at 37°C for 48h, and then cultured by MRS After 2 to 3 subcultures, take 1ml of Lactobacillus reuteri CCFM1178 culture solution, mix it with 9.0mL pH 2.5 artificial simulated gastric juice (MRS medium containing 1% pepsin, pH=2.5), and incubate at 37°C. Under anaerobic culture, samples were taken at 0h, 0.5h, 1h and 2h respectively, poured and cultivated with MRS agar medium, and then plate colonies were counted, and the survival rate was calculated according to the number of viable bacteria. The survival rate is the ratio of the number of viable bacteria when sampling in the culture solution to the number of viable bacteria at the 0th hour, expressed in %.

[0092] Take 1ml of the culture solution of Lactobacillus ...

Embodiment 3

[0097] Example 3: Safety verification of Lactobacillus reuteri CCFM1178 on C57BL / 6J mice

[0098] Resuspend Lactobacillus reuteri CCFM1178 cells in normal saline to make a concentration of 1×10 10 CFU / mL bacterial suspension. Eight healthy male C57BL / 6J mice with a body weight of about 20 g were taken. After one week of adaptation to the environment, 0.2 ml of the bacterial suspension of this concentration was administered orally once a day, observed for one week, and the death and body weight were recorded.

[0099] The results are listed in Table 3. Feeding concentration 1×10 10 CFU / mL of Lactobacillus reuteri CCFM1178 had no significant effect on the mice, the body weight of the mice had no significant change, the appearance of the mice had no obvious pathological symptoms, and no death occurred.

[0100] Table 3 Changes in body weight and death of mice

[0101]

[0102] Note: - means no death of mice.

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Abstract

The invention discloses lactobacillus reuteri CCFM1178 capable of intervening in metabolic syndrome, and application, and belongs to the technical field of microorganisms. The lactobacillus reuteri CCFM1178 screened by the invention can obviously eliminate increase of the serum total cholesterol content and the low-density lipoprotein cholesterol of a mouse with the metabolic syndrome caused by high fat diet, relieve the increase of the body weight of an individual with the metabolic syndrome, and reduce daily diet intake and energy conversion efficiency; the intestinal flora imbalance of the mouse due to high fat is eliminated; and the levels of proinflammatory factors IL-6 and TNF-[alpha] in the liver of the mouse with the metabolic syndrome are improved, and the level of an anti-inflammatory factor IL-10 is improved to prevent the metabolic syndrome, inhibit serum lipopolysaccharide endotoxin and maintain a colon barrier structure. The lactobacillus reuteri CCFM1178 disclosed by the invention can be used for preparing medicines or functional foods for relieving diseases such as the metabolic syndrome, the non-alcoholic fatty liver disease, the diabetes mellitus and the like, and has a very wide application prospect.

Description

technical field [0001] The invention relates to a strain of Lactobacillus reuteri CCFM1178 capable of interfering with metabolic syndrome and its application, belonging to the technical field of microorganisms. Background technique [0002] Metabolic syndrome (MS) is a group of central obesity, hyperglycemia (diabetes or impaired glucose regulation), dyslipidemia (including high TG and / or low HDL-C blood), and hypertension. At the same time, there are multiple risk factors based on abnormal glucose metabolism and lipid metabolism that gather together to promote the occurrence and development of various cardiovascular and cerebrovascular diseases such as type II diabetes and atherosclerosis. In recent years, with the continuous development of my country's economic and social level, the living standards of the public have continued to improve, the proportion of carbohydrates and fats in the diet structure has increased day by day, and the restrictions of many working condition...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P1/00A61P1/16A61P29/00A23C9/123A23C13/16A23C19/032A23C19/06A23L11/50A23L19/00A23L33/135C12R1/225
CPCC12N1/20A61K35/747A61P1/00A61P1/16A61P29/00A23C9/1234A23C13/16A23C19/0323A23C19/062A23L11/50A23L19/00A23L33/135A23V2002/00A23V2400/173A23V2200/332A23V2200/328A23V2200/3262A23V2200/3204A23V2200/30Y02A50/30
Inventor 杨波陈卫郑富莉崔树茂王刚翟齐啸赵建新张灏
Owner JIANGNAN UNIV
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