Injectable hydrogel as well as preparation method and application thereof

A technology for injecting water and hydrogels, applied in the fields of application, pharmaceutical formulation, pharmaceutical science, etc., can solve the problems of less research on mechanical properties of adhesion properties, shorten the gelation time, promote the performance of materials, and improve the possibility of injectable effect

Active Publication Date: 2021-09-21
SHENZHEN PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on how to balance the adhesion and mechanical properties between injectable hydrogels and the body.

Method used

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  • Injectable hydrogel as well as preparation method and application thereof
  • Injectable hydrogel as well as preparation method and application thereof
  • Injectable hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] This embodiment provides an injectable hydrogel, the preparation method of which is as follows:

[0049] (1) Type A pigskin gelatin was dissolved in deionized water to prepare a gelatin solution with a mass fraction of 20 wt%, and the pH was adjusted to 8.0 using Tri buffer.

[0050] (2) Add tannic acid to (1) gelatin solution, the mass fraction of tannic acid in the gelatin solution is 2wt%, stir in 55 ℃ water bath for 6h, prepare the gelatin solution doped with tannic acid.

[0051] (3) Dilute the purified transglutaminase (TG enzyme) in PBS buffer solution to prepare TG enzyme solution, the concentration of TG enzyme is 0.5mg / mL.

[0052] (4) Mix the gelatin solution doped with tannic acid in step (2) and the TG enzyme solution in step (3) with a volume ratio of 5:1, and incubate to obtain the adhesive injectable hydrogel, and the incubation time for 1 min, and the incubation temperature is 37° C. to make it gel, and obtain the injectable hydrogel.

Embodiment 2

[0054] This embodiment provides an injectable hydrogel, the preparation method of which is as follows:

[0055] (1) Type A pigskin gelatin was dissolved in deionized water to prepare a gelatin solution with a mass fraction of 20 wt%, and the pH was adjusted to 8.0 using Tri buffer.

[0056] (2) Add tannic acid to (1) gelatin solution, the mass fraction of tannic acid in the gelatin solution is 4wt%, stir in 55 ℃ water bath for 6h, prepare the gelatin solution doped with tannic acid.

[0057] (3) Dilute the purified transglutaminase (TG enzyme) in PBS buffer solution to prepare TG enzyme solution, the concentration of TG enzyme is 0.5mg / mL.

[0058] (4) Mix the gelatin solution doped with tannic acid in step (2) and the TG enzyme solution in step (3) with a volume ratio of 5:1, and incubate to obtain the adhesive injectable hydrogel, and the incubation time for 40 s, and the incubation temperature was 37° C. to make it gel, and obtain the injectable hydrogel.

Embodiment 3

[0060] This embodiment provides an injectable hydrogel, the preparation method of which is as follows:

[0061] (1) Type A pigskin gelatin was dissolved in deionized water to prepare a gelatin solution with a mass fraction of 10 wt%, and the pH was adjusted to 7.5 using Tri buffer.

[0062] (2) Add tannic acid to (1) gelatin solution, the mass fraction of tannic acid in the gelatin solution is 1wt%, stir in 45 ℃ water bath for 10h, prepare the gelatin solution doped with tannic acid.

[0063] (3) Dilute the purified transglutaminase (TG enzyme) in PBS buffer solution to prepare TG enzyme solution, the concentration of TG enzyme is 1mg / mL.

[0064] (4) Mix the gelatin solution doped with step (2) tannic acid and step (3) TG enzyme solution with a volume ratio of 8:1, and incubate to obtain the adhesive injectable hydrogel, and the incubation time for 40 s, and the incubation temperature was 45° C. to make it gel, and obtain the injectable hydrogel.

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Abstract

The invention relates to injectable hydrogel as well as a preparation method and application thereof. The injectable hydrogel is obtained by taking gelatin and tannic acid as raw materials and carrying out chemical crosslinking under the catalysis of transglutaminase. Gelatin molecular chains in the injectable hydrogel form a network structure under the action of amido bonds, polyphenol hydroxyl groups of the tannic acid and amino groups on the gelatin chains have hydrogen-bond interaction, the polyphenol hydroxyl groups of the tannic acid can be spontaneously complexed under an alkaline condition, and finally the injectable hydrogel is endowed with excellent adhesion performance; the material and tissues are adhered, the performance of the material is promoted, the injectability of the material is improved, the gelling time is shortened, the clinical operation is easy, and the mechanical property is improved. The hydrogel material can realize adhesion and efficient retention of defective tissues, is suitable for filling various irregular-shaped defects, can efficiently promote the performance of the material to play, and realizes repair of the defective tissues.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to an injectable hydrogel and its preparation method and application, in particular to an injectable hydrogel with excellent adhesion and mechanical properties, its preparation method and application. Background technique [0002] Hydrogel is a kind of material with three-dimensional network structure and high water content, which is similar to the structure of extracellular matrix, which can simulate the microenvironment to promote the growth of cell tissue. In recent years, injectable hydrogels derived from natural polymers have become more attractive in biological tissue engineering due to their good biocompatibility and biodegradability. Injectable hydrogels enable the in situ solidification of fluid biomaterials in defects of arbitrary shape, and further exert their specific effects by filling and adhering in the defect. Therefore, the injectable hydro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08J3/075C08L89/00C08L97/00A61L27/22A61L27/50A61L27/52A61L27/58A61L24/10A61L24/00A61K47/42A61K9/06
CPCC08J3/246C08J3/075A61L27/222A61L27/50A61L27/52A61L27/58A61L24/104A61L24/001A61L24/0031A61L24/0042A61K47/42A61K9/06C08J2389/00C08J2497/00A61L2400/06A61L2300/412A61L2430/02
Inventor 彭松林谭宝玉陈欣唐榕泽杨大志
Owner SHENZHEN PEOPLES HOSPITAL
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