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Preparation method of disease-inhibiting soil containing microbial agent and application thereof

A microbial inoculum and soil technology, which is applied in the field of preparation of disease-suppressing soil, can solve problems such as easily causing soil-borne diseases, and achieve the effects of preventing and controlling soil-borne diseases and reducing the possibility and quantity.

Pending Publication Date: 2021-09-24
CHINA TOBACCO CENTRAL SOUTH AGRICULTURAL EXPERIMENTAL STATION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the technical problem that soil-borne diseases are easily caused after continuous cropping, the invention discloses a preparation method of disease-suppressing soil containing microbial agents and its application

Method used

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  • Preparation method of disease-inhibiting soil containing microbial agent and application thereof
  • Preparation method of disease-inhibiting soil containing microbial agent and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0020] A method for preparing disease-suppressing soil containing microbial inoculum, spraying the microbial inoculum evenly on the soil surface, and plowing the soil with a plowing depth of 5 cm;

[0021] The microbial agent contains the following components: 0.8×10 9 cfu / ml Lactobacillus casei (Lactobacillus casei), 1.3×10 9 cfu / ml Lactobacillus plantarum (Lactobacillus plantarum), 0.6×10 9 cfu / ml lactic acid Pediococcus (Pediococcus acidilactici Lindner), 0.7×10 9 cfu / ml of Rhodopseudomonas palustris (Molisch) van Niel and 0.5×10 9 cfu / ml Lactobacillus bulgaricus (Lactobacillus bulgaricus); the dosage of the microbial bacterial agent is 5L / mu.

[0022] A method for preparing microbial inoculum, comprising the following steps:

[0023] (1) Strain preparation: prepare Lactobacillus casei, Lactobacillus plantarum, Pediococcus acidilactici Lindner, Rhodopseudomonas palustris (Molisch) van Niel and Lactobacillus bulgaricus (Lactobacillus bulgaricus);

[0024] (2) Strain ac...

Embodiment 2

[0028] A method for preparing disease-suppressing soil containing microbial inoculum, spraying the microbial inoculum evenly on the soil surface, and plowing the soil with a plowing depth of 25 cm;

[0029] The microbial agent contains the following components: 1.2×10 9 cfu / ml Lactobacillus casei (Lactobacillus casei), 1.5×10 9 cfu / ml Lactobacillus plantarum (Lactobacillus plantarum), 1.0×10 9 cfu / ml Pediococcus acidilactici Lindner, 1.3×10 9 cfu / ml of Rhodopseudomonas palustris (Molisch) van Niel and 1.1 x 10 9 cfu / ml Lactobacillus bulgaricus (Lactobacillus bulgaricus); the amount of microbial bacterial agent is 8L / mu.

[0030] A method for preparing microbial inoculum, comprising the following steps:

[0031] (1) Strain preparation: prepare Lactobacillus casei, Lactobacillus plantarum, Pediococcus acidilactici Lindner, Rhodopseudomonas palustris (Molisch) van Niel and Lactobacillus bulgaricus (Lactobacillus bulgaricus);

[0032] (2) Strain activation: Lactobacillus cas...

Embodiment 3

[0036] A method for preparing disease-suppressing soil containing microbial inoculum, spraying the microbial inoculum evenly on the soil surface, and plowing the soil to a depth of 10 cm;

[0037] The microbial agent contains the following components: 1.0×10 9 cfu / ml Lactobacillus casei (Lactobacillus casei), 1.4×10 9 cfu / ml Lactobacillus plantarum (Lactobacillus plantarum), 0.8×10 9 cfu / ml lactic acid Pediococcus (Pediococcus acidilactici Lindner), 1.2×10 9 cfu / ml of Rhodopseudomonas palustris (Molisch) van Niel and 0.8×10 9 cfu / ml Lactobacillus bulgaricus (Lactobacillus bulgaricus); the dosage of the microbial bacterial agent is 6L / mu.

[0038] A method for preparing microbial inoculum, comprising the following steps:

[0039] (1) Strain preparation: prepare Lactobacillus casei, Lactobacillus plantarum, Pediococcus acidilactici Lindner, Rhodopseudomonas palustris (Molisch) van Niel and Lactobacillus bulgaricus (Lactobacillus bulgaricus);

[0040] (2) Strain activation:...

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Abstract

The invention discloses a preparation method of disease-inhibiting soil containing a microbial agent, which comprises the following steps: uniformly spraying the microbial agent on the surface of the soil, and ploughing the soil at the ploughing depth of 5-25 cm, wherein the microbial agent is prepared from the following components: lactobacillus casei, lactobacillus plantarum, pediococcus acidilactici, rhodopseudomonas palustris and lactobacillus bulgaricus. The invention also discloses application of the soil prepared by the preparation method of the disease-inhibiting soil containing the microbial agent in biological prevention and control in tobacco planting, and the soil-borne diseases of tobacco can be effectively prevented and controlled.

Description

technical field [0001] The invention relates to the technical field of soil improvement, in particular to a method for preparing disease-suppressing soil containing microbial agents and its application. Background technique [0002] The change of soil microbial community is the most important biological factor affecting the occurrence of soil-borne diseases. The bacterial diversity index of soil microbial community tends to decrease due to long-term continuous cropping, and the composition of soil microorganisms transforms from "bacterial type" to "fungal type". In addition, during continuous cropping, crops often release allelopathic autotoxic substances into the environment, and the autotoxic substances secreted by roots have a great impact on the composition of microbial communities in soil. In the "plant, soil, microorganism" triangle interaction relationship, pathogenic bacteria can better utilize the autotoxic substances accumulated by crop continuous cropping, so the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G24/12A01G24/20A01G13/00A01G22/45C12N1/20C12R1/245C12R1/25C12R1/38C12R1/225C12R1/01
CPCA01G24/12A01G24/20A01G13/00A01G22/45C12N1/20
Inventor 刘勇军周志成尹华群孟德龙刘天波杨佳蒴段淑辉
Owner CHINA TOBACCO CENTRAL SOUTH AGRICULTURAL EXPERIMENTAL STATION
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